Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Purification of rectangle DNA origami by rate-zonal centrifugation | |
| Chao, J; Xing, S; Feng, BY; Wang, JB; Dai, JB; Wang, LH; Li, Q; Li, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China. | |
| 2015 | |
| Source Publication | NUCLEAR SCIENCE AND TECHNIQUES
 |
| ISSN | 1001-8042 |
| Volume | 26Issue:5Pages:— |
| Subtype | 期刊文献 |
| Abstract | DNA origami technique, a breakthrough in DNA nanotechnology, has been widely used to prepare complex DNA nanostructures with nanoscale addressability. However, the purity and yield are generally the bottleneck to application of DNA nanostructures, and current methods for purifying DNA origami nanostructures in large quantities are time-consuming and laborious. This study aims to develop a scalable, cost-effective and contamination-free method of purifying DNA origami nanostructures. We employ an effective and convenient purification approach to purify planar rectangle DNA origami structures through rate-zonal centrifugation. By subjecting DNA origami samples to high centrifugal force in a density gradient media of glycerol, well-folded nanostructures and by-products are separated successfully, which are confirmed by agarose gel electrophoresis and atomic force microscopy (AFM). This method will aid the production of pure rectangle DNA origami nanostructures in large quantity. |
| Keyword | Folding Dna Nanoscale Shapes Nanoarchitectures Nanostructures Separation |
| Language | 英语 |
| WOS ID | WOS:000365459200018 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/25023 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Corresponding Author | Li, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China. |
| Recommended Citation GB/T 7714 | Chao, J,Xing, S,Feng, BY,et al. Purification of rectangle DNA origami by rate-zonal centrifugation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2015,26(5):—. |
| APA | Chao, J.,Xing, S.,Feng, BY.,Wang, JB.,Dai, JB.,...&Li, Q .(2015).Purification of rectangle DNA origami by rate-zonal centrifugation.NUCLEAR SCIENCE AND TECHNIQUES,26(5),—. |
| MLA | Chao, J,et al."Purification of rectangle DNA origami by rate-zonal centrifugation".NUCLEAR SCIENCE AND TECHNIQUES 26.5(2015):—. |
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| File Name/Size | DocType | Version | Access | License | ||
| Purification of rect（2361KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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