CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Purification of rectangle DNA origami by rate-zonal centrifugation
Chao, J; Xing, S; Feng, BY; Wang, JB; Dai, JB; Wang, LH; Li, Q; Li, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.
2015
Source PublicationNUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042
Volume26Issue:5Pages:
Subtype期刊文献
AbstractDNA origami technique, a breakthrough in DNA nanotechnology, has been widely used to prepare complex DNA nanostructures with nanoscale addressability. However, the purity and yield are generally the bottleneck to application of DNA nanostructures, and current methods for purifying DNA origami nanostructures in large quantities are time-consuming and laborious. This study aims to develop a scalable, cost-effective and contamination-free method of purifying DNA origami nanostructures. We employ an effective and convenient purification approach to purify planar rectangle DNA origami structures through rate-zonal centrifugation. By subjecting DNA origami samples to high centrifugal force in a density gradient media of glycerol, well-folded nanostructures and by-products are separated successfully, which are confirmed by agarose gel electrophoresis and atomic force microscopy (AFM). This method will aid the production of pure rectangle DNA origami nanostructures in large quantity.
KeywordFolding Dna Nanoscale Shapes Nanoarchitectures Nanostructures Separation
Language英语
WOS IDWOS:000365459200018
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/25023
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorLi, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Chao, J,Xing, S,Feng, BY,et al. Purification of rectangle DNA origami by rate-zonal centrifugation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2015,26(5):—.
APA Chao, J.,Xing, S.,Feng, BY.,Wang, JB.,Dai, JB.,...&Li, Q .(2015).Purification of rectangle DNA origami by rate-zonal centrifugation.NUCLEAR SCIENCE AND TECHNIQUES,26(5),—.
MLA Chao, J,et al."Purification of rectangle DNA origami by rate-zonal centrifugation".NUCLEAR SCIENCE AND TECHNIQUES 26.5(2015):—.
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