CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens
Lou, XD; Zhuang, Y; Zuo, XL; Jia, YM; Hong, YN; Min, XH; Zhang, ZY; Xu, XM; Liu, NN; Xia, F; Tang, BZ; Xia, F (reprint author), Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Key Lab Large Format Battery Mat & Syst, Minist Educ, Wuhan 430074, Peoples R China.
2015
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume87Issue:13Pages:6822—6827
Subtype期刊文献
AbstractAs a biomarker for early cancer diagnosis, telomerase are one of the promising targets for cancer therapeutics. Inspired by the fluorescent emission principle of aggregation-induced emission fluorogens, we creatively designed an AIE-based turn-on method to detect telomerase activity from cell extracts. A positively charged fluorogen (TPE-Z) is not fluorescent when freely diffused in solution. The fluorescence of TPE-Z is enhanced with the elongation of the DNA strand which could light up telomere elongation process. By exploitation of it, we can detect telomerase activity from different cell lines (E-J, HeLa, MCF-7, and HLF) with high sensitivity and specificity. Moreover, our method is successfully employed to demonstrate the applications in bladder cancer diagnosis (41 urine specimens from bladder cancer patients and 15 urine specimens from normal people are detected). The ATE-based method provides a simple one-pot technique for quantification and monitoring of the telomerase activity and shows great potential for future use in clinical tests.
KeywordAggregation-induced Emission Repeat Amplification Protocol Highly Sensitive Detection In-situ Intracellular Telomerase Quantum Dots Living Cells Assay-method Tumor-cells Dna
Language英语
WOS IDWOS:000357839700059
Citation statistics
Cited Times:78[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/25029
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorXia, F (reprint author), Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Key Lab Large Format Battery Mat & Syst, Minist Educ, Wuhan 430074, Peoples R China.
Recommended Citation
GB/T 7714
Lou, XD,Zhuang, Y,Zuo, XL,et al. Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens[J]. ANALYTICAL CHEMISTRY,2015,87(13):6822—6827.
APA Lou, XD.,Zhuang, Y.,Zuo, XL.,Jia, YM.,Hong, YN.,...&Xia, F .(2015).Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens.ANALYTICAL CHEMISTRY,87(13),6822—6827.
MLA Lou, XD,et al."Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens".ANALYTICAL CHEMISTRY 87.13(2015):6822—6827.
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