Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens
Lou, XD; Zhuang, Y; Zuo, XL; Jia, YM; Hong, YN; Min, XH; Zhang, ZY; Xu, XM; Liu, NN; Xia, F; Tang, BZ; Xia, F (reprint author), Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Key Lab Large Format Battery Mat & Syst, Minist Educ, Wuhan 430074, Peoples R China.
2015
发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
卷号87期号:13页码:6822—6827
文章类型期刊文献
摘要As a biomarker for early cancer diagnosis, telomerase are one of the promising targets for cancer therapeutics. Inspired by the fluorescent emission principle of aggregation-induced emission fluorogens, we creatively designed an AIE-based turn-on method to detect telomerase activity from cell extracts. A positively charged fluorogen (TPE-Z) is not fluorescent when freely diffused in solution. The fluorescence of TPE-Z is enhanced with the elongation of the DNA strand which could light up telomere elongation process. By exploitation of it, we can detect telomerase activity from different cell lines (E-J, HeLa, MCF-7, and HLF) with high sensitivity and specificity. Moreover, our method is successfully employed to demonstrate the applications in bladder cancer diagnosis (41 urine specimens from bladder cancer patients and 15 urine specimens from normal people are detected). The ATE-based method provides a simple one-pot technique for quantification and monitoring of the telomerase activity and shows great potential for future use in clinical tests.
关键词Aggregation-induced Emission Repeat Amplification Protocol Highly Sensitive Detection In-situ Intracellular Telomerase Quantum Dots Living Cells Assay-method Tumor-cells Dna
语种英语
WOS记录号WOS:000357839700059
引用统计
被引频次:53[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25029
专题中科院上海应用物理研究所2011-2018年
通讯作者Xia, F (reprint author), Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Key Lab Large Format Battery Mat & Syst, Minist Educ, Wuhan 430074, Peoples R China.
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GB/T 7714
Lou, XD,Zhuang, Y,Zuo, XL,et al. Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens[J]. ANALYTICAL CHEMISTRY,2015,87(13):6822—6827.
APA Lou, XD.,Zhuang, Y.,Zuo, XL.,Jia, YM.,Hong, YN.,...&Xia, F .(2015).Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens.ANALYTICAL CHEMISTRY,87(13),6822—6827.
MLA Lou, XD,et al."Real-Time, Quantitative Lighting-up Detection of Telomerase in Urines of Bladder Cancer Patients by AIEgens".ANALYTICAL CHEMISTRY 87.13(2015):6822—6827.
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