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| Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC | |
| Zeng, HL; Liu, Q; Yu, JG; Wang, ML; Chen, M; Wang, RH; He, X; Gao, MH; Chen, XQ; Chen, XQ (reprint author), Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China. | |
| 2015 | |
| 发表期刊 | JOURNAL OF SEPARATION SCIENCE
 |
| ISSN | 1615-9306 |
| 卷号 | 38期号:22页码:3897—3904 |
| 文章类型 | 期刊文献 |
| 摘要 | In this study, an on-line two-dimensional high-speed counter-current chromatography system based on a six-port valve was developed. Target-guided by ultrafiltration with high-performance liquid chromatography, the one-step isolation of three potential alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench was achieved by employing the developed orthogonal system and extrusion elution mode. The purities of three potential alpha-amylase inhibitors were all over 95% as determined by high-performance liquid chromatography. Furthermore, UV, mass spectrometry and H-1 NMR spectroscopy were applied to the structural identification of the isolated three target compounds, their structures were assigned as quercetin-3-O-sophoroside (i), 5,7,3',4'-tetrahydroxy flavonol-3-O-[beta-D-rhamnopyranosil(1 -> 2)]-beta-D-glucopyranoside (ii) and isoquercitrin (iii), respectively. The Results demonstrated that the proposed method was highly efficient to screen and isolate enzyme inhibitors from complex natural products extracts, and on-line two-dimensional high-speed countercurrent chromatography can effectively increase the peak resolution of target compounds. |
| 关键词 | Performance Liquid-chromatography Immobilized Enzyme Recycling Elution In-vitro Purification Leaves Phase Polysaccharide Identification Optimization |
| 语种 | 英语 |
| WOS记录号 | WOS:000365320800012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.sinap.ac.cn/handle/331007/25036 |
| 专题 | 中科院上海应用物理研究所2011-2018年 |
| 通讯作者 | Chen, XQ (reprint author), Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Zeng, HL,Liu, Q,Yu, JG,et al. Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(22):3897—3904. |
| APA | Zeng, HL.,Liu, Q.,Yu, JG.,Wang, ML.,Chen, M.,...&Chen, XQ .(2015).Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC.JOURNAL OF SEPARATION SCIENCE,38(22),3897—3904. |
| MLA | Zeng, HL,et al."Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC".JOURNAL OF SEPARATION SCIENCE 38.22(2015):3897—3904. |
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