Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC

CAS OpenIR > 中科院上海应用物理研究所2011-2018年

	Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC
	Zeng, HL; Liu, Q; Yu, JG; Wang, ML; Chen, M; Wang, RH; He, X; Gao, MH; Chen, XQ; Chen, XQ (reprint author), Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
	2015
发表期刊	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9306
卷号	38 期号:22 页码:3897—3904
文章类型	期刊文献
摘要	In this study, an on-line two-dimensional high-speed counter-current chromatography system based on a six-port valve was developed. Target-guided by ultrafiltration with high-performance liquid chromatography, the one-step isolation of three potential alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench was achieved by employing the developed orthogonal system and extrusion elution mode. The purities of three potential alpha-amylase inhibitors were all over 95% as determined by high-performance liquid chromatography. Furthermore, UV, mass spectrometry and H-1 NMR spectroscopy were applied to the structural identification of the isolated three target compounds, their structures were assigned as quercetin-3-O-sophoroside (i), 5,7,3',4'-tetrahydroxy flavonol-3-O-[beta-D-rhamnopyranosil(1 -> 2)]-beta-D-glucopyranoside (ii) and isoquercitrin (iii), respectively. The Results demonstrated that the proposed method was highly efficient to screen and isolate enzyme inhibitors from complex natural products extracts, and on-line two-dimensional high-speed countercurrent chromatography can effectively increase the peak resolution of target compounds.
关键词	Performance Liquid-chromatography Immobilized Enzyme Recycling Elution In-vitro Purification Leaves Phase Polysaccharide Identification Optimization
语种	英语
WOS记录号	WOS:000365320800012
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.sinap.ac.cn/handle/331007/25036
专题	中科院上海应用物理研究所2011-2018年
通讯作者	Chen, XQ (reprint author), Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
推荐引用方式 GB/T 7714	Zeng, HL,Liu, Q,Yu, JG,et al. Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(22):3897—3904.
APA	Zeng, HL.,Liu, Q.,Yu, JG.,Wang, ML.,Chen, M.,...&Chen, XQ .(2015).Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC.JOURNAL OF SEPARATION SCIENCE,38(22),3897—3904.
MLA	Zeng, HL,et al."Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC".JOURNAL OF SEPARATION SCIENCE 38.22(2015):3897—3904.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Separation of alpha-（3041KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文