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Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC
Zeng, HL; Liu, Q; Yu, JG; Wang, ML; Chen, M; Wang, RH; He, X; Gao, MH; Chen, XQ; Chen, XQ (reprint author), Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
2015
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume38Issue:22Pages:3897—3904
Subtype期刊文献
AbstractIn this study, an on-line two-dimensional high-speed counter-current chromatography system based on a six-port valve was developed. Target-guided by ultrafiltration with high-performance liquid chromatography, the one-step isolation of three potential alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench was achieved by employing the developed orthogonal system and extrusion elution mode. The purities of three potential alpha-amylase inhibitors were all over 95% as determined by high-performance liquid chromatography. Furthermore, UV, mass spectrometry and H-1 NMR spectroscopy were applied to the structural identification of the isolated three target compounds, their structures were assigned as quercetin-3-O-sophoroside (i), 5,7,3',4'-tetrahydroxy flavonol-3-O-[beta-D-rhamnopyranosil(1 -> 2)]-beta-D-glucopyranoside (ii) and isoquercitrin (iii), respectively. The Results demonstrated that the proposed method was highly efficient to screen and isolate enzyme inhibitors from complex natural products extracts, and on-line two-dimensional high-speed countercurrent chromatography can effectively increase the peak resolution of target compounds.
KeywordPerformance Liquid-chromatography Immobilized Enzyme Recycling Elution In-vitro Purification Leaves Phase Polysaccharide Identification Optimization
Language英语
WOS IDWOS:000365320800012
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/25036
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorChen, XQ (reprint author), Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
Recommended Citation
GB/T 7714
Zeng, HL,Liu, Q,Yu, JG,et al. Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(22):3897—3904.
APA Zeng, HL.,Liu, Q.,Yu, JG.,Wang, ML.,Chen, M.,...&Chen, XQ .(2015).Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC.JOURNAL OF SEPARATION SCIENCE,38(22),3897—3904.
MLA Zeng, HL,et al."Separation of alpha-amylase inhibitors from Abelmoschus esculentus (L). Moench by on-line two-dimensional high-speed counter-current chromatography target-guided by ultrafiltration-HPLC".JOURNAL OF SEPARATION SCIENCE 38.22(2015):3897—3904.
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