Visualizing dopamine released from living cells using a nanoplasmonic probe
Qin, WW; Wang, SP; Li, J; Peng, TH; Xu, Y; Wang, K; Shi, JY; Fan, CH; Li, D; Li, D (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai 201800, Peoples R China.
2015
发表期刊NANOSCALE
ISSN2040-3364
卷号7期号:37页码:15070—15074
文章类型期刊文献
摘要We report the development of an ultrasensitive nanoplasmonic probe for discriminative detection and imaging of dopamine released from living cells. The sensing mechanism is based on the dopamine-induced seeded-growth of Au nanoparticles (Au NPs) that leads to the shift of the plasmon band. This platform allows for the detection of dopamine with a detection limit down to 0.25 pM within 1 min. This nanoplasmonic assay is further applied to visualize the release of dopamine from living rat pheochromocytoma (PC12) cells under ATP-stimulation with dark-field microscopy (DFM). The DFM results together with real time fluorescence imaging of PC12 cells stained with the Fluo calcium indicator, suggested that ATP stimulated-release of dopamine is concomitant with the Ca2+ influx, and the influx of Ca2+ is through ATP-activated channels instead of the voltage-gated Ca2+ channel (VGC).
关键词Single Vesicle Release Au Nanoparticles Neurotransmitter Release Gold Nanoparticles Synaptic Vesicle Pc12 Cells Metal Nanoparticles Growth Microelectrodes Nanosensors
语种英语
WOS记录号WOS:000361675300007
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25096
专题中科院上海应用物理研究所2011-2018年
通讯作者Li, D (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai 201800, Peoples R China.
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GB/T 7714
Qin, WW,Wang, SP,Li, J,et al. Visualizing dopamine released from living cells using a nanoplasmonic probe[J]. NANOSCALE,2015,7(37):15070—15074.
APA Qin, WW.,Wang, SP.,Li, J.,Peng, TH.,Xu, Y.,...&Li, D .(2015).Visualizing dopamine released from living cells using a nanoplasmonic probe.NANOSCALE,7(37),15070—15074.
MLA Qin, WW,et al."Visualizing dopamine released from living cells using a nanoplasmonic probe".NANOSCALE 7.37(2015):15070—15074.
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