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CAS OpenIR  > 中科院上海应用物理研究所2011-2017年  > 期刊论文
题名:
Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure
作者: Mao, XH; Simon, AJ; Pei, H; Shi, JY; Li, J; Huang, Q; Plaxco, KW; Fan, CH
刊名: CHEMICAL SCIENCE
出版日期: 2016
卷号: 7, 期号:2, 页码:1200—1204
关键词: COMPUTATIONAL DESIGN ; PEROXIDASE-ACTIVITY ; PROTEIN ; ORIGAMI ; ASSEMBLIES ; SWITCHES ; INSIGHTS ; RELEASE ; COMPLEX ; SENSORS
DOI: 10.1039/c5sc03705k
通讯作者: Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai, Peoples R China. ; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Bioimaging Ctr, Shanghai, Peoples R China. ; Shi, JY (reprint author), Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England. ; Shi, JY (reprint author), UCB Pharma, 208 Bath Rd, Slough SL1 3WE, Berks, England. ; Fan, CH (reprint author), ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201200, Peoples R China.
文章类型: 期刊文献
英文摘要: Recognition of the fundamental importance of allosteric regulation in biology dates back to not long after its discovery in the 1960s. Our ability to rationally engineer this potentially useful property into normally non-allosteric catalysts, however, remains limited. In response we report a DNA nanotechnology-enabled approach for introducing allostery into catalytic nucleic acids. Specifically, we have grafted one or two copies of a peroxidase-like DNAzyme, hemin-bound G-quadruplex (hemin-G), onto a DNA tetrahedral nanostructure in such a manner as to cause them to interact, modulating their catalytic activity. We achieve allosteric regulation of these catalysts by incorporating dynamically responsive oligonucleotides that respond to specific "effector" molecules (complementary oligonucleotides or small molecules), altering the spacing between the catalytic sites and thus regulating their activity. This designable approach thus enables subtle allosteric modulation in DNAzymes that is potentially of use for nanomedicine and nanomachines.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000368835300045
ISSN号: 2041-6520
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/25641
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Mao, XH,Simon, AJ,Pei, H,et al. Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure[J]. CHEMICAL SCIENCE,2016-01-01,7(2):1200—1204.
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