Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure
Mao, XH; Simon, AJ; Pei, H; Shi, JY; Li, J; Huang, Q; Plaxco, KW; Fan, CH; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Bioimaging Ctr, Shanghai, Peoples R China.; Shi, JY (reprint author), Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England.; Shi, JY (reprint author), UCB Pharma, 208 Bath Rd, Slough SL1 3WE, Berks, England.; Fan, CH (reprint author), ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201200, Peoples R China.
2016
发表期刊CHEMICAL SCIENCE
ISSN2041-6520
卷号7期号:2页码:1200—1204
文章类型期刊文献
摘要Recognition of the fundamental importance of allosteric regulation in biology dates back to not long after its discovery in the 1960s. Our ability to rationally engineer this potentially useful property into normally non-allosteric catalysts, however, remains limited. In response we report a DNA nanotechnology-enabled approach for introducing allostery into catalytic nucleic acids. Specifically, we have grafted one or two copies of a peroxidase-like DNAzyme, hemin-bound G-quadruplex (hemin-G), onto a DNA tetrahedral nanostructure in such a manner as to cause them to interact, modulating their catalytic activity. We achieve allosteric regulation of these catalysts by incorporating dynamically responsive oligonucleotides that respond to specific "effector" molecules (complementary oligonucleotides or small molecules), altering the spacing between the catalytic sites and thus regulating their activity. This designable approach thus enables subtle allosteric modulation in DNAzymes that is potentially of use for nanomedicine and nanomachines.
关键词Computational Design Peroxidase-activity Protein Origami Assemblies Switches Insights Release Complex Sensors
DOI10.1039/c5sc03705k
收录类别SCI
语种英语
WOS记录号WOS:000368835300045
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25641
专题中科院上海应用物理研究所2011-2018年
通讯作者Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Bioimaging Ctr, Shanghai, Peoples R China.; Shi, JY (reprint author), Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England.; Shi, JY (reprint author), UCB Pharma, 208 Bath Rd, Slough SL1 3WE, Berks, England.; Fan, CH (reprint author), ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201200, Peoples R China.
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Mao, XH,Simon, AJ,Pei, H,et al. Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure[J]. CHEMICAL SCIENCE,2016,7(2):1200—1204.
APA Mao, XH.,Simon, AJ.,Pei, H.,Shi, JY.,Li, J.,...&Fan, CH .(2016).Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure.CHEMICAL SCIENCE,7(2),1200—1204.
MLA Mao, XH,et al."Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure".CHEMICAL SCIENCE 7.2(2016):1200—1204.
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