Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure

doi:10.1039/c5sc03705k

CAS OpenIR > 中科院上海应用物理研究所2011-2018年

	Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure
	Mao, XH; Simon, AJ; Pei, H; Shi, JY; Li, J; Huang, Q; Plaxco, KW; Fan, CH; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Bioimaging Ctr, Shanghai, Peoples R China.; Shi, JY (reprint author), Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England.; Shi, JY (reprint author), UCB Pharma, 208 Bath Rd, Slough SL1 3WE, Berks, England.; Fan, CH (reprint author), ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201200, Peoples R China.
	2016
发表期刊	CHEMICAL SCIENCE
ISSN	2041-6520
卷号	7 期号:2 页码:1200—1204
文章类型	期刊文献
摘要	Recognition of the fundamental importance of allosteric regulation in biology dates back to not long after its discovery in the 1960s. Our ability to rationally engineer this potentially useful property into normally non-allosteric catalysts, however, remains limited. In response we report a DNA nanotechnology-enabled approach for introducing allostery into catalytic nucleic acids. Specifically, we have grafted one or two copies of a peroxidase-like DNAzyme, hemin-bound G-quadruplex (hemin-G), onto a DNA tetrahedral nanostructure in such a manner as to cause them to interact, modulating their catalytic activity. We achieve allosteric regulation of these catalysts by incorporating dynamically responsive oligonucleotides that respond to specific "effector" molecules (complementary oligonucleotides or small molecules), altering the spacing between the catalytic sites and thus regulating their activity. This designable approach thus enables subtle allosteric modulation in DNAzymes that is potentially of use for nanomedicine and nanomachines.
关键词	Computational Design Peroxidase-activity Protein Origami Assemblies Switches Insights Release Complex Sensors
DOI	10.1039/c5sc03705k
收录类别	SCI
语种	英语
WOS记录号	WOS:000368835300045
引用统计
文献类型	期刊论文
条目标识符	http://ir.sinap.ac.cn/handle/331007/25641
专题	中科院上海应用物理研究所2011-2018年
通讯作者	Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Bioimaging Ctr, Shanghai, Peoples R China.; Shi, JY (reprint author), Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England.; Shi, JY (reprint author), UCB Pharma, 208 Bath Rd, Slough SL1 3WE, Berks, England.; Fan, CH (reprint author), ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201200, Peoples R China.
推荐引用方式 GB/T 7714	Mao, XH,Simon, AJ,Pei, H,et al. Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure[J]. CHEMICAL SCIENCE,2016,7(2):1200—1204.
APA	Mao, XH.,Simon, AJ.,Pei, H.,Shi, JY.,Li, J.,...&Fan, CH .(2016).Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure.CHEMICAL SCIENCE,7(2),1200—1204.
MLA	Mao, XH,et al."Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure".CHEMICAL SCIENCE 7.2(2016):1200—1204.

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