Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate
Wang, PJ; Wan, Y; Ali, A; Deng, SY; Su, Y; Fan, CH; Yang, SL; Wan, Y (reprint author), Nanjing Univ Sci & Technol, Sch Mech Engn, Nanjing 210094, Jiangsu, Peoples R China.; Yang, SL (reprint author), Nanjing Univ Sci & Technol, Sch Environm & Biol & Engn, Nanjing 210094, Jiangsu, Peoples R China.
2016
发表期刊SCIENCE CHINA-CHEMISTRY
ISSN1674-7291
卷号59期号:2页码:237—242
文章类型期刊文献
摘要Organophosphorous pesticide (OP) contamination could have serious adverse effects on human health and the environment. Due to the toxicity of OPs and the threat presented by their accidental or intentional release in populated areas, the determination and monitoring of these OPs in food products and environment is of great importance. OPs are present in very small quantities and therefore, methods for their detection need to be highly sensitive and selective. Here, we aimed to develop a simple and selective aptamer-based colorimetric assay for the detection of omethoate, which is one of the commonly used OPs. The principle of the assay is that single-stranded DNA (ssDNA)-wrapped gold nanoparticles (AuNPs) are resistant to salt-induced aggregation. By employing an "artificial antibody" organophosphorous pesticide-binding aptamer (OBA) as the recognition element, aptamer-wrapped AuNPs (Au-apta) show high selectivity towards omethoate, resulting in the disconnection of aptamers from AuNPs and the aggregation of AuNPs. As there is a significant color change from the interparticle plasmon coupling during the aggregation of AuNPs, the established assay showed good linearity between 0.1 and 10 mu mol/L, with a low detection limit of 0.1 mu mol/L. Other OPs such as profenofos, phorate, and isocarbophos would not interfere with the detection of omethoate despite having similar structures. Thus, the colorimetric method shows potential for use in the detection of omethoate in real soil samples.
关键词Chromatography-mass-spectrometry Organophosphorous Pesticides Carbon Nanotubes Rapid Detection Acetylcholinesterase Biosensors Insecticide Residues Exposure Samples
DOI10.1007/s11426-015-5488-5
收录类别SCI
语种英语
WOS记录号WOS:000369346000017
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25649
专题中科院上海应用物理研究所2011-2018年
通讯作者Wan, Y; Wan, Y (reprint author), Nanjing Univ Sci & Technol, Sch Mech Engn, Nanjing 210094, Jiangsu, Peoples R China.; Yang, SL (reprint author), Nanjing Univ Sci & Technol, Sch Environm & Biol & Engn, Nanjing 210094, Jiangsu, Peoples R China.
推荐引用方式
GB/T 7714
Wang, PJ,Wan, Y,Ali, A,et al. Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate[J]. SCIENCE CHINA-CHEMISTRY,2016,59(2):237—242.
APA Wang, PJ.,Wan, Y.,Ali, A.,Deng, SY.,Su, Y.,...&Yang, SL .(2016).Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate.SCIENCE CHINA-CHEMISTRY,59(2),237—242.
MLA Wang, PJ,et al."Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate".SCIENCE CHINA-CHEMISTRY 59.2(2016):237—242.
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