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题名:
Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate
作者: Wang, PJ; Wan, Y; Ali, A; Deng, SY; Su, Y; Fan, CH; Yang, SL
刊名: SCIENCE CHINA-CHEMISTRY
出版日期: 2016
卷号: 59, 期号:2, 页码:237—242
关键词: CHROMATOGRAPHY-MASS-SPECTROMETRY ; ORGANOPHOSPHOROUS PESTICIDES ; CARBON NANOTUBES ; RAPID DETECTION ; ACETYLCHOLINESTERASE ; BIOSENSORS ; INSECTICIDE ; RESIDUES ; EXPOSURE ; SAMPLES
DOI: 10.1007/s11426-015-5488-5
通讯作者: Wan, Y (reprint author), Nanjing Univ Sci & Technol, Sch Mech Engn, Nanjing 210094, Jiangsu, Peoples R China. ; Wan, Y ; Yang, SL (reprint author), Nanjing Univ Sci & Technol, Sch Environm & Biol & Engn, Nanjing 210094, Jiangsu, Peoples R China.
文章类型: 期刊文献
英文摘要: Organophosphorous pesticide (OP) contamination could have serious adverse effects on human health and the environment. Due to the toxicity of OPs and the threat presented by their accidental or intentional release in populated areas, the determination and monitoring of these OPs in food products and environment is of great importance. OPs are present in very small quantities and therefore, methods for their detection need to be highly sensitive and selective. Here, we aimed to develop a simple and selective aptamer-based colorimetric assay for the detection of omethoate, which is one of the commonly used OPs. The principle of the assay is that single-stranded DNA (ssDNA)-wrapped gold nanoparticles (AuNPs) are resistant to salt-induced aggregation. By employing an "artificial antibody" organophosphorous pesticide-binding aptamer (OBA) as the recognition element, aptamer-wrapped AuNPs (Au-apta) show high selectivity towards omethoate, resulting in the disconnection of aptamers from AuNPs and the aggregation of AuNPs. As there is a significant color change from the interparticle plasmon coupling during the aggregation of AuNPs, the established assay showed good linearity between 0.1 and 10 mu mol/L, with a low detection limit of 0.1 mu mol/L. Other OPs such as profenofos, phorate, and isocarbophos would not interfere with the detection of omethoate despite having similar structures. Thus, the colorimetric method shows potential for use in the detection of omethoate in real soil samples.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000369346000017
ISSN号: 1674-7291
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/25649
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Wang, PJ,Wan, Y,Ali, A,et al. Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate[J]. SCIENCE CHINA-CHEMISTRY,2016-01-01,59(2):237—242.
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