Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect

doi:10.1016/j.actbio.2015.12.006

CAS OpenIR > 中科院上海应用物理研究所2011-2018年

	Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect
	Tang, W; Lin, D; Yu, YM; Niu, HY; Guo, H; Yuan, Y; Liu, CS; Liu, CS (reprint author), E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomat, Shanghai 200237, Peoples R China.
	2016
发表期刊	ACTA BIOMATERIALIA
ISSN	1742-7061
卷号	32 期号:-页码:309—323
文章类型	期刊文献
摘要	Critical size bone defects raise great demands for efficient bone substitutes. Mimicking the hierarchical porous architecture and specific biological cues of natural bone has been considered as an effective strategy to facilitate bone regeneration. Herein, a trimodal macro/micro/nano-porous scaffold loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2) was developed. With mesoporous bioactive glass (MBG) as matrix, a trimodal MBG scaffold (TMS) with enhanced compressive strength (4.28 MPa, porosity of 80%) was prepared by a "viscosity controlling" and "homogeneous particle reinforcing" multi-template process. A 7.5 nm, 3D cubic (Im3m) mesoporous structure was tailored for a "size-matched entrapment" of rhBMP-2 to achieve sustained release and preserved bioactivity. RhBMP-2-loaded TMS (TMS/rhBMP-2) induced excellent cell attachment, ingrowth and osteogenesis in vitro. Further in vivo ectopic bone formation and orthotopic rabbit radius critical size defect results indicated that compared to the rhBMP-2-loaded bimodal macro/micro- and macro/nano-porous scaffolds, TMS/rhBMP-2 exhibited appealing bone regeneration capacity. Particularly, in critical size defect, complete bone reconstruction with rapid medullary cavity reunion and sclerotin maturity was observed on TMS/rhBMP-2. On the basis of these results, TMS/rhBMP-2 developed here represents a promising bone substitute for clinical application and the concepts proposed in this study might provide new thoughts on development of future orthopedic biomaterials. Statement of Significance Limited self-regenerating capacity of human body makes the reconstruction of critical size bone defect a significant challenge. Current bone substitutes often exhibit undesirable therapeutic efficacy due to poor osteoconductivity or low osteoinductivity. Herein, TMS/rhBMP-2, an advanced mesoporous bioactive glass (MBG) scaffold with osteoconductive trimodal macro/micro/nano-porosity and osteoinductive rhBMP-2 delivery was developed. The preparative and mechanical problems of hierarchical MBG scaffold were solved without affecting its excellent biocompatibilities, and rhBMP-2 immobilization in size matched mesopores was first explored. Combining structural and biological cues, TMS/rhBMP-2 achieved a complete regeneration with rapid medullary cavity reunion and sclerotin maturity in rabbit radius critical size defects. The design conceptions proposed in this study might provide new thoughts on development of future orthopedic biomaterials. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
关键词	Bioactive Glass Scaffolds Enhanced Osseointegration Tissue Regeneration Delivery-systems Growth-factors Repair Angiogenesis Biomaterials Osteogenesis Composites
DOI	10.1016/j.actbio.2015.12.006
收录类别	SCI
语种	英语
WOS记录号	WOS:000371649100030
引用统计
文献类型	期刊论文
条目标识符	http://ir.sinap.ac.cn/handle/331007/25655
专题	中科院上海应用物理研究所2011-2018年
通讯作者	Yuan, Y; Liu, CS (reprint author), E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomat, Shanghai 200237, Peoples R China.
推荐引用方式 GB/T 7714	Tang, W,Lin, D,Yu, YM,et al. Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect[J]. ACTA BIOMATERIALIA,2016,32(-):309—323.
APA	Tang, W.,Lin, D.,Yu, YM.,Niu, HY.,Guo, H.,...&Liu, CS .(2016).Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect.ACTA BIOMATERIALIA,32(-),309—323.
MLA	Tang, W,et al."Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect".ACTA BIOMATERIALIA 32.-(2016):309—323.

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