Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect
Tang, W; Lin, D; Yu, YM; Niu, HY; Guo, H; Yuan, Y; Liu, CS; Liu, CS (reprint author), E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomat, Shanghai 200237, Peoples R China.
2016
发表期刊ACTA BIOMATERIALIA
ISSN1742-7061
卷号32期号:-页码:309—323
文章类型期刊文献
摘要Critical size bone defects raise great demands for efficient bone substitutes. Mimicking the hierarchical porous architecture and specific biological cues of natural bone has been considered as an effective strategy to facilitate bone regeneration. Herein, a trimodal macro/micro/nano-porous scaffold loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2) was developed. With mesoporous bioactive glass (MBG) as matrix, a trimodal MBG scaffold (TMS) with enhanced compressive strength (4.28 MPa, porosity of 80%) was prepared by a "viscosity controlling" and "homogeneous particle reinforcing" multi-template process. A 7.5 nm, 3D cubic (Im3m) mesoporous structure was tailored for a "size-matched entrapment" of rhBMP-2 to achieve sustained release and preserved bioactivity. RhBMP-2-loaded TMS (TMS/rhBMP-2) induced excellent cell attachment, ingrowth and osteogenesis in vitro. Further in vivo ectopic bone formation and orthotopic rabbit radius critical size defect results indicated that compared to the rhBMP-2-loaded bimodal macro/micro- and macro/nano-porous scaffolds, TMS/rhBMP-2 exhibited appealing bone regeneration capacity. Particularly, in critical size defect, complete bone reconstruction with rapid medullary cavity reunion and sclerotin maturity was observed on TMS/rhBMP-2. On the basis of these results, TMS/rhBMP-2 developed here represents a promising bone substitute for clinical application and the concepts proposed in this study might provide new thoughts on development of future orthopedic biomaterials. Statement of Significance Limited self-regenerating capacity of human body makes the reconstruction of critical size bone defect a significant challenge. Current bone substitutes often exhibit undesirable therapeutic efficacy due to poor osteoconductivity or low osteoinductivity. Herein, TMS/rhBMP-2, an advanced mesoporous bioactive glass (MBG) scaffold with osteoconductive trimodal macro/micro/nano-porosity and osteoinductive rhBMP-2 delivery was developed. The preparative and mechanical problems of hierarchical MBG scaffold were solved without affecting its excellent biocompatibilities, and rhBMP-2 immobilization in size matched mesopores was first explored. Combining structural and biological cues, TMS/rhBMP-2 achieved a complete regeneration with rapid medullary cavity reunion and sclerotin maturity in rabbit radius critical size defects. The design conceptions proposed in this study might provide new thoughts on development of future orthopedic biomaterials. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
关键词Bioactive Glass Scaffolds Enhanced Osseointegration Tissue Regeneration Delivery-systems Growth-factors Repair Angiogenesis Biomaterials Osteogenesis Composites
DOI10.1016/j.actbio.2015.12.006
收录类别SCI
语种英语
WOS记录号WOS:000371649100030
引用统计
被引频次:48[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25655
专题中科院上海应用物理研究所2011-2018年
通讯作者Yuan, Y; Liu, CS (reprint author), E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomat, Shanghai 200237, Peoples R China.
推荐引用方式
GB/T 7714
Tang, W,Lin, D,Yu, YM,et al. Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect[J]. ACTA BIOMATERIALIA,2016,32(-):309—323.
APA Tang, W.,Lin, D.,Yu, YM.,Niu, HY.,Guo, H.,...&Liu, CS .(2016).Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect.ACTA BIOMATERIALIA,32(-),309—323.
MLA Tang, W,et al."Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect".ACTA BIOMATERIALIA 32.-(2016):309—323.
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