DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers
Wang, SP; Cai, XQ; Wang, LH; Li, J; Li, Q; Zuo, XL; Shi, JY; Huang, Q; Fan, CH; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.
2016
发表期刊CHEMICAL SCIENCE
ISSN2041-6520
卷号7期号:4页码:2722—2727
文章类型期刊文献
摘要To better understand cell behaviors on substrates, the precise control of density and orientation of cell-specific ligands remains a great challenge. In this study, we established an easy-to-use approach to manipulate the adhesion and patterning of mammalian cells on gold substrates. We prepared DNA self-assembled monolayers (DNA-SAMs) on gold substrates and found that the sequence-specific orientation of DNA-SAMs played an important role in modulating cell adhesion. We also found that the DNA-SAMs on gold substrates could be used as a potentially universal cell culture substrate, which showed properties similar to cationic polymers (e.g. poly-L lysine, PLL) substrates. Furthermore, we could manipulate cell adhesion by tuning the length of poly adenine (polyA) in the DNA sequence. We also prepared a DNA aptamer-based SAM to regulate cell adhesion by exploiting stimuli-responsive conformational change of the aptamer. By using the well-established DNA spotting technology, we patterned cells on DNA-SAMs to form a spot matrix and four English letters "CELL". Our findings suggest that DNA-SAMs on gold substrates are potentially useful for making smart surfaces for cell studies, thus introducing a new platform for cell/tissue engineering research.
关键词Single-stranded-dna Cancer-cells Surface Gold Forces Microenvironment Differentiation Gradients Molecules Integrin
DOI10.1039/c5sc04102c
收录类别SCI
语种英语
WOS记录号WOS:000372614800034
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25690
专题中科院上海应用物理研究所2011-2018年
通讯作者Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.
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Wang, SP,Cai, XQ,Wang, LH,et al. DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers[J]. CHEMICAL SCIENCE,2016,7(4):2722—2727.
APA Wang, SP.,Cai, XQ.,Wang, LH.,Li, J.,Li, Q.,...&Zuo, XL .(2016).DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers.CHEMICAL SCIENCE,7(4),2722—2727.
MLA Wang, SP,et al."DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers".CHEMICAL SCIENCE 7.4(2016):2722—2727.
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