中国科学院上海应用物理研究所机构知识库
Advanced  
CAS OpenIR  > 中科院上海应用物理研究所2011-2017年  > 期刊论文
题名:
DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers
作者: Wang, SP; Cai, XQ; Wang, LH; Li, J; Li, Q; Zuo, XL; Shi, JY; Huang, Q; Fan, CH
刊名: CHEMICAL SCIENCE
出版日期: 2016
卷号: 7, 期号:4, 页码:2722—2727
关键词: SINGLE-STRANDED-DNA ; CANCER-CELLS ; SURFACE ; GOLD ; FORCES ; MICROENVIRONMENT ; DIFFERENTIATION ; GRADIENTS ; MOLECULES ; INTEGRIN
DOI: 10.1039/c5sc04102c
通讯作者: Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China. ; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.
文章类型: 期刊文献
英文摘要: To better understand cell behaviors on substrates, the precise control of density and orientation of cell-specific ligands remains a great challenge. In this study, we established an easy-to-use approach to manipulate the adhesion and patterning of mammalian cells on gold substrates. We prepared DNA self-assembled monolayers (DNA-SAMs) on gold substrates and found that the sequence-specific orientation of DNA-SAMs played an important role in modulating cell adhesion. We also found that the DNA-SAMs on gold substrates could be used as a potentially universal cell culture substrate, which showed properties similar to cationic polymers (e.g. poly-L lysine, PLL) substrates. Furthermore, we could manipulate cell adhesion by tuning the length of poly adenine (polyA) in the DNA sequence. We also prepared a DNA aptamer-based SAM to regulate cell adhesion by exploiting stimuli-responsive conformational change of the aptamer. By using the well-established DNA spotting technology, we patterned cells on DNA-SAMs to form a spot matrix and four English letters "CELL". Our findings suggest that DNA-SAMs on gold substrates are potentially useful for making smart surfaces for cell studies, thus introducing a new platform for cell/tissue engineering research.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000372614800034
ISSN号: 2041-6520
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/25690
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers.pdf(2138KB)期刊论文作者接受稿开放获取View Download

Recommended Citation:
Wang, SP,Cai, XQ,Wang, LH,et al. DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers[J]. CHEMICAL SCIENCE,2016-01-01,7(4):2722—2727.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, SP]'s Articles
[Cai, XQ]'s Articles
[Wang, LH]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, SP]‘s Articles
[Cai, XQ]‘s Articles
[Wang, LH]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace