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题名:
Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability
作者: Yao, SK; Fan, JD; Zong, YB; He, Y; Zhou, GZ; Sun, ZB; Zhang, JH; Huang, QJ; Xiao, TQ; Jiang, HD
刊名: APPLIED PHYSICS LETTERS
出版日期: 2016
卷号: 108, 期号:12, 页码:
关键词: PHASE-CONTRAST ; TOMOGRAPHY ; MICROSCOPY ; RECONSTRUCTION ; RETRIEVAL
DOI: 10.1063/1.4944727
通讯作者: Jiang, HD (reprint author), Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China.
文章类型: 期刊文献
英文摘要: Three-dimensional X-ray imaging of living specimens is challenging due to the limited resolution of conventional absorption contrast X-ray imaging and potential irradiation damage of biological specimens. In this letter, we present microtomography of a living specimen combining phase-contrast imaging and a Fourier-based iterative algorithm termed equally sloped tomography. Non-destructive 3D imaging of an anesthetized living yellow mealworm Tenebrio molitor was demonstrated with a relatively low dose using synchrotron generated X-rays. Based on the high-quality 3D images, branching tracheoles and different tissues of the insect in a natural state were identified and analyzed, demonstrating a significant advantage of the technique over conventional X-ray radiography or histotomy. Additionally, the insect survived without problem after a 1.92-s X-ray exposure and subsequent absorbed radiation dose of similar to 1.2 Gy. No notable physiological effects were observed after reviving the insect from anesthesia. The improved static tomographic method demonstrated in this letter shows advantage in the non-destructive structural investigation of living insects in three dimensions due to the low radiation dose and high resolution capability, and offers many potential applications in biological science. (C) 2016 AIP Publishing LLC.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000373348000045
ISSN号: 0003-6951
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/25718
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Yao, SK,Fan, JD,Zong, YB,et al. Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability[J]. APPLIED PHYSICS LETTERS,2016-01-01,108(12):—.
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