Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability | |
| Yao, SK; Fan, JD; Zong, YB; He, Y; Zhou, GZ; Sun, ZB; Zhang, JH; Huang, QJ; Xiao, TQ; Jiang, HD; Jiang, HD (reprint author), Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China. | |
| 2016 | |
| Source Publication | APPLIED PHYSICS LETTERS
 |
| ISSN | 0003-6951 |
| Volume | 108Issue:12Pages:— |
| Subtype | 期刊文献 |
| Abstract | Three-dimensional X-ray imaging of living specimens is challenging due to the limited resolution of conventional absorption contrast X-ray imaging and potential irradiation damage of biological specimens. In this letter, we present microtomography of a living specimen combining phase-contrast imaging and a Fourier-based iterative algorithm termed equally sloped tomography. Non-destructive 3D imaging of an anesthetized living yellow mealworm Tenebrio molitor was demonstrated with a relatively low dose using synchrotron generated X-rays. Based on the high-quality 3D images, branching tracheoles and different tissues of the insect in a natural state were identified and analyzed, demonstrating a significant advantage of the technique over conventional X-ray radiography or histotomy. Additionally, the insect survived without problem after a 1.92-s X-ray exposure and subsequent absorbed radiation dose of similar to 1.2 Gy. No notable physiological effects were observed after reviving the insect from anesthesia. The improved static tomographic method demonstrated in this letter shows advantage in the non-destructive structural investigation of living insects in three dimensions due to the low radiation dose and high resolution capability, and offers many potential applications in biological science. (C) 2016 AIP Publishing LLC. |
| Keyword | Phase-contrast Tomography Microscopy Reconstruction Retrieval |
| DOI | 10.1063/1.4944727 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000373348000045 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/25718 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Corresponding Author | Jiang, HD (reprint author), Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China. |
| Recommended Citation GB/T 7714 | Yao, SK,Fan, JD,Zong, YB,et al. Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability[J]. APPLIED PHYSICS LETTERS,2016,108(12):—. |
| APA | Yao, SK.,Fan, JD.,Zong, YB.,He, Y.,Zhou, GZ.,...&Jiang, HD .(2016).Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability.APPLIED PHYSICS LETTERS,108(12),—. |
| MLA | Yao, SK,et al."Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability".APPLIED PHYSICS LETTERS 108.12(2016):—. |
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| File Name/Size | DocType | Version | Access | License | ||
| Equally sloped X-ray（1810KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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