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Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability
Yao, SK; Fan, JD; Zong, YB; He, Y; Zhou, GZ; Sun, ZB; Zhang, JH; Huang, QJ; Xiao, TQ; Jiang, HD; Jiang, HD (reprint author), Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China.
2016
Source PublicationAPPLIED PHYSICS LETTERS
ISSN0003-6951
Volume108Issue:12Pages:
Subtype期刊文献
AbstractThree-dimensional X-ray imaging of living specimens is challenging due to the limited resolution of conventional absorption contrast X-ray imaging and potential irradiation damage of biological specimens. In this letter, we present microtomography of a living specimen combining phase-contrast imaging and a Fourier-based iterative algorithm termed equally sloped tomography. Non-destructive 3D imaging of an anesthetized living yellow mealworm Tenebrio molitor was demonstrated with a relatively low dose using synchrotron generated X-rays. Based on the high-quality 3D images, branching tracheoles and different tissues of the insect in a natural state were identified and analyzed, demonstrating a significant advantage of the technique over conventional X-ray radiography or histotomy. Additionally, the insect survived without problem after a 1.92-s X-ray exposure and subsequent absorbed radiation dose of similar to 1.2 Gy. No notable physiological effects were observed after reviving the insect from anesthesia. The improved static tomographic method demonstrated in this letter shows advantage in the non-destructive structural investigation of living insects in three dimensions due to the low radiation dose and high resolution capability, and offers many potential applications in biological science. (C) 2016 AIP Publishing LLC.
KeywordPhase-contrast Tomography Microscopy Reconstruction Retrieval
DOI10.1063/1.4944727
Indexed BySCI
Language英语
WOS IDWOS:000373348000045
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/25718
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorJiang, HD (reprint author), Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China.
Recommended Citation
GB/T 7714
Yao, SK,Fan, JD,Zong, YB,et al. Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability[J]. APPLIED PHYSICS LETTERS,2016,108(12):—.
APA Yao, SK.,Fan, JD.,Zong, YB.,He, Y.,Zhou, GZ.,...&Jiang, HD .(2016).Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability.APPLIED PHYSICS LETTERS,108(12),—.
MLA Yao, SK,et al."Equally sloped X-ray microtomography of living insects with low radiation dose and improved resolution capability".APPLIED PHYSICS LETTERS 108.12(2016):—.
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