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Evidence of cell surface iron speciation of acidophilic iron-oxidizing microorganisms in indirect bioleaching process
Nie, ZY; Liu, HC; Xia, JL; Yang, Y; Zhen, XJ; Zhang, LJ; Qiu, GZ; Xia, JL (reprint author), Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China.; Xia, JL (reprint author), Cent S Univ, Key Lab Biomet, Minist Educ China, Changsha 410083, Hunan, Peoples R China.
2016
Source PublicationBIOMETALS
ISSN0966-0844
Volume29Issue:1Pages:25—37
Subtype期刊文献
AbstractWhile indirect model has been widely accepted in bioleaching, but the evidence of cell surface iron speciation has not been reported. In the present work the iron speciation on the cell surfaces of four typically acidophilic iron-oxidizing microorganism (mesophilic Acidithiobacillus ferrooxidans ATCC 23270, moderately thermophilic Leptospirillum ferriphilum YSK and Sulfobacillus thermosulfidooxidans St, and extremely thermophilic Acidianus manzaensis YN25) grown on different energy substrates (chalcopyrite, pyrite, ferrous sulfate and elemental sulfur (S-0)) were studied in situ firstly by using synchrotron-based micro- X-ray fluorescence analysis and X-ray absorption near-edge structure spectroscopy. Results showed that the cells grown on iron-containing substrates had apparently higher surface iron content than the cells grown on S-0. Both ferrous iron and ferric iron were detected on the cell surface of all tested AIOMs, and the Fe(II)/Fe(III) ratios of the same microorganism were affected by different energy substrates. The iron distribution and bonding state of single cell of A. manzaensis were then studied in situ by scanning transmission soft X-ray microscopy based on dual-energy contrast analysis and stack analysis. Results showed that the iron species distributed evenly on the cell surface and bonded with amino, carboxyl and hydroxyl groups.
KeywordExtracellular Polymeric Substances Ray-absorption-spectroscopy Near-edge Spectra Acidithiobacillus-ferrooxidans Sulfobacillus-thermosulfidooxidans Differential Utilization Sulfur Chalcopyrite Mechanisms Bacteria
DOI10.1007/s10534-015-9893-1
Indexed BySCI
Language英语
WOS IDWOS:000369309400003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/25719
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorXia, JL (reprint author), Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China.; Xia, JL (reprint author), Cent S Univ, Key Lab Biomet, Minist Educ China, Changsha 410083, Hunan, Peoples R China.
Recommended Citation
GB/T 7714
Nie, ZY,Liu, HC,Xia, JL,et al. Evidence of cell surface iron speciation of acidophilic iron-oxidizing microorganisms in indirect bioleaching process[J]. BIOMETALS,2016,29(1):25—37.
APA Nie, ZY.,Liu, HC.,Xia, JL.,Yang, Y.,Zhen, XJ.,...&Xia, JL .(2016).Evidence of cell surface iron speciation of acidophilic iron-oxidizing microorganisms in indirect bioleaching process.BIOMETALS,29(1),25—37.
MLA Nie, ZY,et al."Evidence of cell surface iron speciation of acidophilic iron-oxidizing microorganisms in indirect bioleaching process".BIOMETALS 29.1(2016):25—37.
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