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题名:
High-Sensitivity and High-Efficiency Detection of DNA Hydroxymethylation in Genomic DNA by Multiplexing Electrochemical Biosensing
作者: Chen, SX; Dou, YZ; Zhao, ZH; Li, FW; Su, J; Fan, CH; Song, SP
刊名: ANALYTICAL CHEMISTRY
出版日期: 2016
卷号: 88, 期号:7, 页码:3476—3480
关键词: PRINTED AMPEROMETRIC BIOSENSOR ; MAMMALIAN DNA ; TET PROTEINS ; 5-HYDROXYMETHYLCYTOSINE ; METHYLATION ; 5-METHYLCYTOSINE ; BRAIN ; 5-CARBOXYLCYTOSINE ; QUANTIFICATION ; CANCER
DOI: 10.1021/acs.analchem.6b00230
通讯作者: Fan, CH ; Song, SP (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China. ; Fan, CH ; Song, SP (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.
文章类型: 期刊文献
英文摘要: DNA hydroxymethylation (5-hmC) is a kind of new epigenetic modification, which plays key roles in DNA demethylation, genomic reprogramming, and the gene expression in mammals. For further exploring the functions of 5-hmC, it is necessary to develop sensitive and selective methods for detecting 5-hmC. Herein, we developed a novel multiplexing electrochemical (MEC) biosensor for 5-hmC detection based on the glycosylation modification of 5-hmC and enzymatic signal amplification. The 5-hmC was first glycosylated by T4 beta-glucosyltransferase and then oxidated by sodium periodate. The resulting glucosyl-modified 5-hmC (5-ghmC) was incubated with ARP-biotin and was bound to avidin-HRP. The 5-hmC can be detected at the subnanogram level. Finally, we performed 5-hmC detection for mouse tissue samples and cancer cell lines. The limit of detection of the MEC biosensor is 20 times lower than that of commercial kits based on optical meaurement. Also, the biosensor presented high detection specificity because the chemical reaction for 5-hmC modification can not happen at any other unhydroxymethylated nucleic acid bases. Importantly, benefited by its multiplexing capacity, the developed MEC biosensor showed excellent high efficiency, which was time-saving and cost less.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000373656300011
ISSN号: 0003-2700
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/25746
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Chen, SX,Dou, YZ,Zhao, ZH,et al. High-Sensitivity and High-Efficiency Detection of DNA Hydroxymethylation in Genomic DNA by Multiplexing Electrochemical Biosensing[J]. ANALYTICAL CHEMISTRY,2016-01-01,88(7):3476—3480.
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