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Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays
Chao, J; Li, ZH; Li, J; Peng, HZ; Su, S; Li, Q; Zhu, CF; Zuo, XL; Song, SP; Wang, LH; Wang, LH; Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, 2019 Jialuo Rd, Shanghai 201800, Peoples R China.; Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, 2019 Jialuo Rd, Shanghai 201800, Peoples R China.
2016
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume81Issue:-Pages:92—96
Subtype期刊文献
AbstractMicroarrays of biomolecules hold great promise in the fields of genomics, proteomics, and clinical assays on account of their remarkably parallel and high-throughput assay capability. However, the fluorescence detection used in most conventional DNA microarrays is still limited by sensitivity. In this study, we have demonstrated a novel universal and highly sensitive platform for fluorescent detection of sequence specific DNA at the femtomolar level by combining dextran-coated microarrays with hybridization chain reaction (HCR) signal amplification. Three-dimensional dextran matrix was covalently coated on glass surface as the scaffold to immobilize DNA recognition probes to increase the surface binding capacity and accessibility. DNA nanowire tentacles were formed on the matrix surface for efficient signal amplification by capturing multiple fluorescent molecules in a highly ordered way. By quantifying microscopic fluorescent signals, the synergetic effects of dextran and HCR greatly improved sensitivity of DNA microarrays, with a detection limit of 10 fM (1 x 10(5) molecules). This detection assay could recognize one-base mismatch with fluorescence signals dropped down to 20%. This cost-effective microarray platform also worked well with samples in serum and thus shows great potential for clinical diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
KeywordSurface-plasmon Resonance Cancer-patients On-chip Microrna Cells Immobilization Nanoparticles Biosensors Universal Platform
DOI10.1016/j.bios.2016.01.093
Indexed BySCI
Language英语
WOS IDWOS:000374811800014
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/25747
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorWang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, 2019 Jialuo Rd, Shanghai 201800, Peoples R China.; Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, 2019 Jialuo Rd, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Chao, J,Li, ZH,Li, J,et al. Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays[J]. BIOSENSORS & BIOELECTRONICS,2016,81(-):92—96.
APA Chao, J.,Li, ZH.,Li, J.,Peng, HZ.,Su, S.,...&Wang, LH .(2016).Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays.BIOSENSORS & BIOELECTRONICS,81(-),92—96.
MLA Chao, J,et al."Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays".BIOSENSORS & BIOELECTRONICS 81.-(2016):92—96.
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