Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays
Chao, J; Li, ZH; Li, J; Peng, HZ; Su, S; Li, Q; Zhu, CF; Zuo, XL; Song, SP; Wang, LH; Wang, LH; Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, 2019 Jialuo Rd, Shanghai 201800, Peoples R China.; Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, 2019 Jialuo Rd, Shanghai 201800, Peoples R China.
2016
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号81期号:-页码:92—96
文章类型期刊文献
摘要Microarrays of biomolecules hold great promise in the fields of genomics, proteomics, and clinical assays on account of their remarkably parallel and high-throughput assay capability. However, the fluorescence detection used in most conventional DNA microarrays is still limited by sensitivity. In this study, we have demonstrated a novel universal and highly sensitive platform for fluorescent detection of sequence specific DNA at the femtomolar level by combining dextran-coated microarrays with hybridization chain reaction (HCR) signal amplification. Three-dimensional dextran matrix was covalently coated on glass surface as the scaffold to immobilize DNA recognition probes to increase the surface binding capacity and accessibility. DNA nanowire tentacles were formed on the matrix surface for efficient signal amplification by capturing multiple fluorescent molecules in a highly ordered way. By quantifying microscopic fluorescent signals, the synergetic effects of dextran and HCR greatly improved sensitivity of DNA microarrays, with a detection limit of 10 fM (1 x 10(5) molecules). This detection assay could recognize one-base mismatch with fluorescence signals dropped down to 20%. This cost-effective microarray platform also worked well with samples in serum and thus shows great potential for clinical diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
关键词Surface-plasmon Resonance Cancer-patients On-chip Microrna Cells Immobilization Nanoparticles Biosensors Universal Platform
DOI10.1016/j.bios.2016.01.093
收录类别SCI
语种英语
WOS记录号WOS:000374811800014
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25747
专题中科院上海应用物理研究所2011-2018年
通讯作者Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol, 2019 Jialuo Rd, Shanghai 201800, Peoples R China.; Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, 2019 Jialuo Rd, Shanghai 201800, Peoples R China.
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GB/T 7714
Chao, J,Li, ZH,Li, J,et al. Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays[J]. BIOSENSORS & BIOELECTRONICS,2016,81(-):92—96.
APA Chao, J.,Li, ZH.,Li, J.,Peng, HZ.,Su, S.,...&Wang, LH .(2016).Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays.BIOSENSORS & BIOELECTRONICS,81(-),92—96.
MLA Chao, J,et al."Hybridization chain reaction amplification for highly sensitive fluorescence detection of DNA with dextran coated microarrays".BIOSENSORS & BIOELECTRONICS 81.-(2016):92—96.
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