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Mechanical force-induced polymerization and depolymerization of F-actin at water/solid interfaces
Zhang, XQ; Hu, XY; Lei, HZ; Hu, J; Zhang, Y; Zhang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China.
2016
Source PublicationNANOSCALE
ISSN2040-3364
Volume8Issue:11Pages:6008—6013
Subtype期刊文献
AbstractActin molecules are among the three main cytoskeleton proteins of cells and undergo rapid cycling to regulate critical processes such as endocytosis, cytokinesis, cell polarity, and cell morphogenesis. Although extensive studies have been carried out on the dynamics as well as biological functions of actin polymerization and depolymerization both in vivo and in vitro, the molecular mechanisms by which cells sense and respond to mechanical signals are not fully understood. In particular, little attention has been paid to the effect of a physical force that is exerted directly on the actin cytoskeleton. In this paper, we have explored how the mechanical force affects the actin polymerization and depolymerization behaviors at water/solid interfaces using an atomic force microscope (AFM) operated in liquid. By raster scanning an AFM probe on a substrate surface with a certain load, it was found that actin monomers could polymerize into filaments without the help of actin related proteins (ARPs). Further study indicated that actin monomers were inclined to form filaments only under a small scanning load. The polymerized actin filaments would be depolymerized when the mechanical force was stronger. A possible mechanism has been suggested to explain the mechanical force induced actin polymerization.
KeywordFocal Adhesions Motility Driven Cell Motility Filaments Microscopy Cytoskeleton Nanofibers Nucleation Integrins Complexes
DOI10.1039/c5nr08713a
Indexed BySCI
Language英语
WOS IDWOS:000372245900026
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/25770
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorZhang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Zhang, XQ,Hu, XY,Lei, HZ,et al. Mechanical force-induced polymerization and depolymerization of F-actin at water/solid interfaces[J]. NANOSCALE,2016,8(11):6008—6013.
APA Zhang, XQ,Hu, XY,Lei, HZ,Hu, J,Zhang, Y,&Zhang, Y .(2016).Mechanical force-induced polymerization and depolymerization of F-actin at water/solid interfaces.NANOSCALE,8(11),6008—6013.
MLA Zhang, XQ,et al."Mechanical force-induced polymerization and depolymerization of F-actin at water/solid interfaces".NANOSCALE 8.11(2016):6008—6013.
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