Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing

doi:10.1016/j.bios.2015.12.076

CAS OpenIR > 中科院上海应用物理研究所2011-2018年

	Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing
	Li, Y; Zhang, Z; Zhang, YT; Deng, DM; Luo, LQ; Han, BS; Fan, CH; Luo, LQ (reprint author), Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China.; Han, BS (reprint author), Shanghai Jiao Tong Univ, Shanghai Xinhua Hosp, Dept Surg, Shanghai 200092, Peoples R China.
	2016
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	79 期号:-页码:536—542
文章类型	期刊文献
摘要	A novel protocol of label-free electrochemical impedance immunosensor based on bovine serum albumin-nitidine chloride-reduced graphene oxide (BSA-NC-rGO) nanocomposite was proposed for quantitative determination of carcino-embryonic antigen (CEA). BSA was anchored to rGO via the aromatic plane of NC by pi-stacking interaction to realize bio-functionalization of rGO, and then gold nanoparticles (AuNPs) were electrodeposited onto the surface of BSA-NC-rGO nanocomposite. The morphology, conductivity and interaction of different nanocomposites were characterized by scanning electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and UV-vis spectrum. CEA monoclonal antibody (anti-CEA) was conjugated to AuNPs via gold-thiol chemistry to construct electrochemical immunosensing platform, and the specific immunoreaction between CEA and anti-CEA was monitored by EIS. Under optimum conditions, CEA could be quantified in a wide range of 0.1-200 ng mL(-1) (R=0.9948) with low detection limit of 0.067 ng mL(-1). The proposed immunosensor exhibited great potential for detecting blood samples. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Carbon Nanotubes Carcinoembryonic Antigen Impedance Spectroscopy Gold Nanoparticles Biomarker Detection Signaling Pathway Binding Cancer Nanocomposite Immunoassay
DOI	10.1016/j.bios.2015.12.076
收录类别	SCI
语种	英语
WOS记录号	WOS:000370309200075
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.sinap.ac.cn/handle/331007/25786
专题	中科院上海应用物理研究所2011-2018年
通讯作者	Luo, LQ (reprint author), Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China.; Han, BS (reprint author), Shanghai Jiao Tong Univ, Shanghai Xinhua Hosp, Dept Surg, Shanghai 200092, Peoples R China.
推荐引用方式 GB/T 7714	Li, Y,Zhang, Z,Zhang, YT,et al. Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing[J]. BIOSENSORS & BIOELECTRONICS,2016,79(-):536—542.
APA	Li, Y.,Zhang, Z.,Zhang, YT.,Deng, DM.,Luo, LQ.,...&Han, BS .(2016).Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing.BIOSENSORS & BIOELECTRONICS,79(-),536—542.
MLA	Li, Y,et al."Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing".BIOSENSORS & BIOELECTRONICS 79.-(2016):536—542.

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