Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing
Li, Y; Zhang, Z; Zhang, YT; Deng, DM; Luo, LQ; Han, BS; Fan, CH; Luo, LQ (reprint author), Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China.; Han, BS (reprint author), Shanghai Jiao Tong Univ, Shanghai Xinhua Hosp, Dept Surg, Shanghai 200092, Peoples R China.
2016
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号79期号:-页码:536—542
文章类型期刊文献
摘要A novel protocol of label-free electrochemical impedance immunosensor based on bovine serum albumin-nitidine chloride-reduced graphene oxide (BSA-NC-rGO) nanocomposite was proposed for quantitative determination of carcino-embryonic antigen (CEA). BSA was anchored to rGO via the aromatic plane of NC by pi-stacking interaction to realize bio-functionalization of rGO, and then gold nanoparticles (AuNPs) were electrodeposited onto the surface of BSA-NC-rGO nanocomposite. The morphology, conductivity and interaction of different nanocomposites were characterized by scanning electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and UV-vis spectrum. CEA monoclonal antibody (anti-CEA) was conjugated to AuNPs via gold-thiol chemistry to construct electrochemical immunosensing platform, and the specific immunoreaction between CEA and anti-CEA was monitored by EIS. Under optimum conditions, CEA could be quantified in a wide range of 0.1-200 ng mL(-1) (R=0.9948) with low detection limit of 0.067 ng mL(-1). The proposed immunosensor exhibited great potential for detecting blood samples. (C) 2015 Elsevier B.V. All rights reserved.
关键词Carbon Nanotubes Carcinoembryonic Antigen Impedance Spectroscopy Gold Nanoparticles Biomarker Detection Signaling Pathway Binding Cancer Nanocomposite Immunoassay
DOI10.1016/j.bios.2015.12.076
收录类别SCI
语种英语
WOS记录号WOS:000370309200075
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25786
专题中科院上海应用物理研究所2011-2018年
通讯作者Luo, LQ (reprint author), Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China.; Han, BS (reprint author), Shanghai Jiao Tong Univ, Shanghai Xinhua Hosp, Dept Surg, Shanghai 200092, Peoples R China.
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Li, Y,Zhang, Z,Zhang, YT,et al. Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing[J]. BIOSENSORS & BIOELECTRONICS,2016,79(-):536—542.
APA Li, Y.,Zhang, Z.,Zhang, YT.,Deng, DM.,Luo, LQ.,...&Han, BS .(2016).Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing.BIOSENSORS & BIOELECTRONICS,79(-),536—542.
MLA Li, Y,et al."Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing".BIOSENSORS & BIOELECTRONICS 79.-(2016):536—542.
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