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CAS OpenIR  > 中科院上海应用物理研究所2011-2017年  > 期刊论文
题名:
Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing
作者: Li, Y; Zhang, Z; Zhang, YT; Deng, DM; Luo, LQ; Han, BS; Fan, CH
刊名: BIOSENSORS & BIOELECTRONICS
出版日期: 2016
卷号: 79, 期号:-, 页码:536—542
关键词: CARBON NANOTUBES ; CARCINOEMBRYONIC ANTIGEN ; IMPEDANCE SPECTROSCOPY ; GOLD NANOPARTICLES ; BIOMARKER DETECTION ; SIGNALING PATHWAY ; BINDING ; CANCER ; NANOCOMPOSITE ; IMMUNOASSAY
DOI: 10.1016/j.bios.2015.12.076
通讯作者: Luo, LQ (reprint author), Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China. ; Han, BS (reprint author), Shanghai Jiao Tong Univ, Shanghai Xinhua Hosp, Dept Surg, Shanghai 200092, Peoples R China.
文章类型: 期刊文献
英文摘要: A novel protocol of label-free electrochemical impedance immunosensor based on bovine serum albumin-nitidine chloride-reduced graphene oxide (BSA-NC-rGO) nanocomposite was proposed for quantitative determination of carcino-embryonic antigen (CEA). BSA was anchored to rGO via the aromatic plane of NC by pi-stacking interaction to realize bio-functionalization of rGO, and then gold nanoparticles (AuNPs) were electrodeposited onto the surface of BSA-NC-rGO nanocomposite. The morphology, conductivity and interaction of different nanocomposites were characterized by scanning electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and UV-vis spectrum. CEA monoclonal antibody (anti-CEA) was conjugated to AuNPs via gold-thiol chemistry to construct electrochemical immunosensing platform, and the specific immunoreaction between CEA and anti-CEA was monitored by EIS. Under optimum conditions, CEA could be quantified in a wide range of 0.1-200 ng mL(-1) (R=0.9948) with low detection limit of 0.067 ng mL(-1). The proposed immunosensor exhibited great potential for detecting blood samples. (C) 2015 Elsevier B.V. All rights reserved.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000370309200075
ISSN号: 0956-5663
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/25786
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Li, Y,Zhang, Z,Zhang, YT,et al. Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing[J]. BIOSENSORS & BIOELECTRONICS,2016-01-01,79(-):536—542.
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