Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing

doi:10.1016/j.bios.2015.12.076

CAS OpenIR > 中科院上海应用物理研究所2011-2020年

	Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing
	Li, Y; Zhang, Z; Zhang, YT; Deng, DM; Luo, LQ; Han, BS; Fan, CH; Luo, LQ (reprint author), Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China.; Han, BS (reprint author), Shanghai Jiao Tong Univ, Shanghai Xinhua Hosp, Dept Surg, Shanghai 200092, Peoples R China.
	2016
Source Publication	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
Volume	79 Issue:-Pages:536—542
Subtype	期刊文献
Abstract	A novel protocol of label-free electrochemical impedance immunosensor based on bovine serum albumin-nitidine chloride-reduced graphene oxide (BSA-NC-rGO) nanocomposite was proposed for quantitative determination of carcino-embryonic antigen (CEA). BSA was anchored to rGO via the aromatic plane of NC by pi-stacking interaction to realize bio-functionalization of rGO, and then gold nanoparticles (AuNPs) were electrodeposited onto the surface of BSA-NC-rGO nanocomposite. The morphology, conductivity and interaction of different nanocomposites were characterized by scanning electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and UV-vis spectrum. CEA monoclonal antibody (anti-CEA) was conjugated to AuNPs via gold-thiol chemistry to construct electrochemical immunosensing platform, and the specific immunoreaction between CEA and anti-CEA was monitored by EIS. Under optimum conditions, CEA could be quantified in a wide range of 0.1-200 ng mL(-1) (R=0.9948) with low detection limit of 0.067 ng mL(-1). The proposed immunosensor exhibited great potential for detecting blood samples. (C) 2015 Elsevier B.V. All rights reserved.
Keyword	Carbon Nanotubes Carcinoembryonic Antigen Impedance Spectroscopy Gold Nanoparticles Biomarker Detection Signaling Pathway Binding Cancer Nanocomposite Immunoassay
DOI	10.1016/j.bios.2015.12.076
Indexed By	SCI
Language	英语
WOS ID	WOS:000370309200075
Citation statistics	Cited Times:19[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/25786
Collection	中科院上海应用物理研究所2011-2020年
Corresponding Author	Luo, LQ (reprint author), Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China.; Han, BS (reprint author), Shanghai Jiao Tong Univ, Shanghai Xinhua Hosp, Dept Surg, Shanghai 200092, Peoples R China.
Recommended Citation GB/T 7714	Li, Y,Zhang, Z,Zhang, YT,et al. Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing[J]. BIOSENSORS & BIOELECTRONICS,2016,79(-):536—542.
APA	Li, Y.,Zhang, Z.,Zhang, YT.,Deng, DM.,Luo, LQ.,...&Han, BS .(2016).Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing.BIOSENSORS & BIOELECTRONICS,79(-),536—542.
MLA	Li, Y,et al."Nitidine chloride-assisted bio-functionalization of reduced graphene oxide by bovine serum albumin for impedimetric immunosensing".BIOSENSORS & BIOELECTRONICS 79.-(2016):536—542.

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