Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice
Li, JB; Jiang, DW; Bao, BL; He, YL; Liu, L; Wang, XM; Wang, XM (reprint author), Inner Mongolia Med Univ, Dept Nucl Med, Affiliated Hosp, 1 Tongdao North St, Hohhot 010050, Peoples R China.
2016
发表期刊BIOCONJUGATE CHEMISTRY
ISSN1043-1802
卷号27期号:4页码:905—910
文章类型期刊文献
摘要Self-assembled DNA nanostructures, as a new type of nanocarriers, have shown great potential in molecular imaging probes. DNA bipyramid nanostructures (DBNs), as a classic kind of DNA nanostructure, can be precisely constructed through the unparalleled base-pairing precision of oligonucleotide strands. DBNs were prepared by self-assembly of six oligonucleotides with equal molar amounts in a single annealing step, and purified by high performance liquid chromatography (HPLC). DBNs were stable in 10% FBS as well as 80% mouse serum for at least 8 h. To prepare Tc-99m-labeled DBNs, N-hydroxysuccinimidyl S-acetylmercaptoacetyl triglycinate (Sacetyl-MAG3-NHS ester) tagged single-stranded DNA (ssDNA) was first radiolabeled with Tc-99m, and DBNs with an overhang were assembled and then hybridized with Tc-99m-ssDNA to prepare Tc-99m-labeled DNA bipyramid nanostructures (Tc-99m-MAG3-DBNs). DBNs were radiolabeled, with the radiochemical purity being over 90%. The plasma half-life of Tc-99m-MAG3-DBNs in normal KM mice was about 6 min. The biodistribution and SPECT/CT imaging were conducted with Tc-99m-MAG3-DBNs in KM mice, and both showed that Tc-99m-MAG3-DBNs mainly concentrated in the intestine, liver, and kidneys, and there was also prominent uptake in the gallbladder and bladder. We successfully obtained a new class of SPECT molecular probes based on this DNA polyhedron structure.
关键词In-vivo Preclinical Evaluation Nanostructures Delivery Platform Cancer Cells Pet
DOI10.1021/acs.bioconjchem.5b00680
收录类别SCI
语种英语
WOS记录号WOS:000374812600008
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25822
专题中科院上海应用物理研究所2011-2018年
通讯作者Li, JB; Wang, XM (reprint author), Inner Mongolia Med Univ, Dept Nucl Med, Affiliated Hosp, 1 Tongdao North St, Hohhot 010050, Peoples R China.
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GB/T 7714
Li, JB,Jiang, DW,Bao, BL,et al. Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice[J]. BIOCONJUGATE CHEMISTRY,2016,27(4):905—910.
APA Li, JB.,Jiang, DW.,Bao, BL.,He, YL.,Liu, L.,...&Wang, XM .(2016).Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice.BIOCONJUGATE CHEMISTRY,27(4),905—910.
MLA Li, JB,et al."Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice".BIOCONJUGATE CHEMISTRY 27.4(2016):905—910.
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