Bian, LM (reprint author), Prince Wales Hosp, Dept Mech & Automat Engn, Div Biomed Engn, Hong Kong, Hong Kong, Peoples R China.
; Bian, LM (reprint author), Prince Wales Hosp, Shun Hing Inst Adv Engn, Hong Kong, Hong Kong, Peoples R China.
; Bian, LM (reprint author), Chinese Univ Hong Kong, Shenzhen Res Inst, Shatin, Hong Kong, Peoples R China.
; Bian, LM (reprint author), China Orthoped Regenerat Med Grp CORMed, Shanghai, Peoples R China.
文章类型:
期刊文献
英文摘要:
Biopolymer-based supramolecular hydrogels cross-linked by host guest interactions are usually mechanically weak as shown in "inverted vials" instead of freestanding 3D constructs. Herein, we describe a novel host guest macromer (HGM) approach for preparation of biopolymer-based freestanding supramolecular hydrogels. Host guest macromers are formed by molecular self-assembly between adamantane-functionalized hyaluronic acid (AD(x)HA) guest polymers and monoacrylated beta-cyclodextrins (mono-Ac-beta CD) host monomers. 'Supramolecular hydrogels are readily prepared by UV-induced polymerization of the preassembled host guest macromers. Such hydrogels are soely cross-linked by in situ formed multivalent host guest nanoclusters and show significantly reinforced mechanical properties yet still retain desirable supramolecular features. They can self-heal and be remolded into freestanding 3D constructs which afford effective protection on the encapsulated stem cells during the compression remolding, making them promising carriers for therapeutic cells that can quickly adapt to and integrate with surrounding tissues of the targeted defects. We demonstrate that such hydrogels not only sustain extended release of encapsulated proteinaceous growth factors (TGF-beta 1) but also support chondrogenesis of the human mesenchymal stem cells (hMSCs) and promote cartilage regeneration in a rat model.
Robust Biopolymeric Supramolecular Host-Guest Macromer Hydrogels Reinforced by in Situ Formed Multivalent Nanoclusters for Cartilage Regeneration.pdf（7506KB）
