Single molecule atomic force microscopy of aerolysin pore complexes reveals unexpected star-shaped topography
He, JF; Wang, JB; Hu, J; Sun, JL; Czajkowsky, DM; Shao, ZF; Sun, JL (reprint author), Shanghai Jiao Tong Univ, Syst Biomed, 800 Dongchuan Rd, Shanghai, Peoples R China.; Czajkowsky, DM (reprint author), Shanghai Jiao Tong Univ, Sch Biomed Engn, 800 Dongchuan Rd, Shanghai, Peoples R China.
2016
发表期刊JOURNAL OF MOLECULAR RECOGNITION
ISSN0952-3499
卷号29期号:4页码:174—181
文章类型期刊文献
摘要Aerolysin is the paradigmatic member of a large family of toxins that convert from a water-soluble monomer/dimer into a membrane-spanning oligomeric pore. While there is x-ray crystallographic data of its water-soluble conformation, the most recent structural model of the membrane-inserted pore is based primarily on data of water-soluble tetradecamers of mutant protein, together with computational modeling ultimately performed in vacuum. Here we examine this pore model with atomic force microscopy (AFM) of membrane-associated wild-type complexes and all-atom molecular dynamics (MD) simulations in water. In striking contrast to a disc-shaped cap region predicted by the present model, the AFM images reveal a star-shaped complex, with a central ring surrounded by seven radial projections. Further, the MD simulations suggest that the locations of the receptor-binding (D1) domains in the present model are not correct. However, a modified model in which the D1 domains, rather than localized at fixed positions, adopt a wide range of configurations through fluctuations of an intervening linker is compatible with existing data. Thus our work not only demonstrates the importance of directly resolving such complexes in their native environment but also points to a dynamic receptor binding region, which may be critical for toxin assembly on the cell surface. Copyright (c) 2015 John Wiley & Sons, Ltd.
关键词Forming Toxin Aerolysin Biological Applications Receptor-binding Membrane-channel Alpha-toxin Dynamics Surface Afm Heptamerization Proaerolysin
DOI10.1002/jmr.2517
收录类别SCI
语种英语
WOS记录号WOS:000372642700006
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/25833
专题中科院上海应用物理研究所2011-2018年
通讯作者Sun, JL (reprint author), Shanghai Jiao Tong Univ, Syst Biomed, 800 Dongchuan Rd, Shanghai, Peoples R China.; Czajkowsky, DM (reprint author), Shanghai Jiao Tong Univ, Sch Biomed Engn, 800 Dongchuan Rd, Shanghai, Peoples R China.
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GB/T 7714
He, JF,Wang, JB,Hu, J,et al. Single molecule atomic force microscopy of aerolysin pore complexes reveals unexpected star-shaped topography[J]. JOURNAL OF MOLECULAR RECOGNITION,2016,29(4):174—181.
APA He, JF.,Wang, JB.,Hu, J.,Sun, JL.,Czajkowsky, DM.,...&Czajkowsky, DM .(2016).Single molecule atomic force microscopy of aerolysin pore complexes reveals unexpected star-shaped topography.JOURNAL OF MOLECULAR RECOGNITION,29(4),174—181.
MLA He, JF,et al."Single molecule atomic force microscopy of aerolysin pore complexes reveals unexpected star-shaped topography".JOURNAL OF MOLECULAR RECOGNITION 29.4(2016):174—181.
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