中国科学院上海应用物理研究所机构知识库
Advanced  
CAS OpenIR  > 中科院上海应用物理研究所2011-2017年  > 期刊论文
题名:
Single molecule atomic force microscopy of aerolysin pore complexes reveals unexpected star-shaped topography
作者: He, JF; Wang, JB; Hu, J; Sun, JL; Czajkowsky, DM; Shao, ZF
刊名: JOURNAL OF MOLECULAR RECOGNITION
出版日期: 2016
卷号: 29, 期号:4, 页码:174—181
关键词: FORMING TOXIN AEROLYSIN ; BIOLOGICAL APPLICATIONS ; RECEPTOR-BINDING ; MEMBRANE-CHANNEL ; ALPHA-TOXIN ; DYNAMICS ; SURFACE ; AFM ; HEPTAMERIZATION ; PROAEROLYSIN
DOI: 10.1002/jmr.2517
通讯作者: Sun, JL (reprint author), Shanghai Jiao Tong Univ, Syst Biomed, 800 Dongchuan Rd, Shanghai, Peoples R China. ; Czajkowsky, DM (reprint author), Shanghai Jiao Tong Univ, Sch Biomed Engn, 800 Dongchuan Rd, Shanghai, Peoples R China.
文章类型: 期刊文献
英文摘要: Aerolysin is the paradigmatic member of a large family of toxins that convert from a water-soluble monomer/dimer into a membrane-spanning oligomeric pore. While there is x-ray crystallographic data of its water-soluble conformation, the most recent structural model of the membrane-inserted pore is based primarily on data of water-soluble tetradecamers of mutant protein, together with computational modeling ultimately performed in vacuum. Here we examine this pore model with atomic force microscopy (AFM) of membrane-associated wild-type complexes and all-atom molecular dynamics (MD) simulations in water. In striking contrast to a disc-shaped cap region predicted by the present model, the AFM images reveal a star-shaped complex, with a central ring surrounded by seven radial projections. Further, the MD simulations suggest that the locations of the receptor-binding (D1) domains in the present model are not correct. However, a modified model in which the D1 domains, rather than localized at fixed positions, adopt a wide range of configurations through fluctuations of an intervening linker is compatible with existing data. Thus our work not only demonstrates the importance of directly resolving such complexes in their native environment but also points to a dynamic receptor binding region, which may be critical for toxin assembly on the cell surface. Copyright (c) 2015 John Wiley & Sons, Ltd.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000372642700006
ISSN号: 0952-3499
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/25833
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Single molecule atomic force microscopy of aerolysin pore complexes reveals unexpected star-shaped topography.pdf(4120KB)期刊论文作者接受稿开放获取View Download

Recommended Citation:
He, JF,Wang, JB,Hu, J,et al. Single molecule atomic force microscopy of aerolysin pore complexes reveals unexpected star-shaped topography[J]. JOURNAL OF MOLECULAR RECOGNITION,2016-01-01,29(4):174—181.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[He, JF]'s Articles
[Wang, JB]'s Articles
[Hu, J]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[He, JF]‘s Articles
[Wang, JB]‘s Articles
[Hu, J]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Single molecule atomic force microscopy of aerolysin pore complexes reveals unexpected star-shaped topography.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace