CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Structural investigation into physiological DNA phosphorothioate modification
Lan, WX; Hu, ZP; Shen, J; Wang, CX; Jiang, F; Liu, HL; Long, DW; Liu, ML; Cao, CY; Cao, CY (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China.
2016
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume6Issue:-Pages:
Subtype期刊文献
AbstractDNA phosphorothioate (PT) modification, with sulfur replacing a nonbridging phosphate oxygen in a sequence and stereo specific manner, is a novel physiological variation in bacteria. But what effects on DNA properties PT modification has is still unclear. To address this, we prepared three double-stranded (ds) DNA decamers, d(CG(PX)GCCGCCGA) with its complementary strand d(TCGGCG(PX)GCCG) (where X = O or S, i.e., PT-free dsDNA, [S-p, S-p]-PT dsDNA or [R-p, R-p]-PT dsDNA) located in gene of Streptomyces lividans. Their melting temperature (T-m) measurement indicates that [R-p, R-p]-PT dsDNA is most unstable. Their electron transfer potential detection presents an order of anti-oxidation properties: S-p-PT DNA > R-p-PT DNA > PT-free DNA. Their NMR structures demonstrate that PT modification doesn't change their B-form conformation. The sulfur in [R-p, R-p]-PT dsDNA locates in the major groove, with steric effects on protons in the sugar close to modification sites, resulting in its unstability, and facilitating its selectively interactions with ScoMcrA. We thought that PT modification was dialectical to the bacteria. It protects the hosting bacteria by working as antioxidant against H2O2, and acts as a marker, directing restriction enzyme observed in other hosts, like ScoMcrA, to correctly cleave the PT modified DNA, so that bacteria cannot spread and survive.
KeywordStreptomyces-lividans Escherichia-coli Nmr-spectroscopy Protein Stability Bacteria Spectra System Site Iron
DOI10.1038/srep25737
Indexed BySCI
Language英语
WOS IDWOS:000375672600001
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/25844
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorCao, CY (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China.
Recommended Citation
GB/T 7714
Lan, WX,Hu, ZP,Shen, J,et al. Structural investigation into physiological DNA phosphorothioate modification[J]. SCIENTIFIC REPORTS,2016,6(-):—.
APA Lan, WX.,Hu, ZP.,Shen, J.,Wang, CX.,Jiang, F.,...&Cao, CY .(2016).Structural investigation into physiological DNA phosphorothioate modification.SCIENTIFIC REPORTS,6(-),—.
MLA Lan, WX,et al."Structural investigation into physiological DNA phosphorothioate modification".SCIENTIFIC REPORTS 6.-(2016):—.
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