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题名:
Structural investigation into physiological DNA phosphorothioate modification
作者: Lan, WX; Hu, ZP; Shen, J; Wang, CX; Jiang, F; Liu, HL; Long, DW; Liu, ML; Cao, CY
刊名: SCIENTIFIC REPORTS
出版日期: 2016
卷号: 6, 期号:-, 页码:
关键词: STREPTOMYCES-LIVIDANS ; ESCHERICHIA-COLI ; NMR-SPECTROSCOPY ; PROTEIN ; STABILITY ; BACTERIA ; SPECTRA ; SYSTEM ; SITE ; IRON
DOI: 10.1038/srep25737
通讯作者: Cao, CY (reprint author), Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China.
文章类型: 期刊文献
英文摘要: DNA phosphorothioate (PT) modification, with sulfur replacing a nonbridging phosphate oxygen in a sequence and stereo specific manner, is a novel physiological variation in bacteria. But what effects on DNA properties PT modification has is still unclear. To address this, we prepared three double-stranded (ds) DNA decamers, d(CG(PX)GCCGCCGA) with its complementary strand d(TCGGCG(PX)GCCG) (where X = O or S, i.e., PT-free dsDNA, [S-p, S-p]-PT dsDNA or [R-p, R-p]-PT dsDNA) located in gene of Streptomyces lividans. Their melting temperature (T-m) measurement indicates that [R-p, R-p]-PT dsDNA is most unstable. Their electron transfer potential detection presents an order of anti-oxidation properties: S-p-PT DNA > R-p-PT DNA > PT-free DNA. Their NMR structures demonstrate that PT modification doesn't change their B-form conformation. The sulfur in [R-p, R-p]-PT dsDNA locates in the major groove, with steric effects on protons in the sugar close to modification sites, resulting in its unstability, and facilitating its selectively interactions with ScoMcrA. We thought that PT modification was dialectical to the bacteria. It protects the hosting bacteria by working as antioxidant against H2O2, and acts as a marker, directing restriction enzyme observed in other hosts, like ScoMcrA, to correctly cleave the PT modified DNA, so that bacteria cannot spread and survive.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000375672600001
ISSN号: 2045-2322
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/25844
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Lan, WX,Hu, ZP,Shen, J,et al. Structural investigation into physiological DNA phosphorothioate modification[J]. SCIENTIFIC REPORTS,2016-01-01,6(-):—.
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