Automatic crystal centring procedure at the SSRF macromolecular crystallography beamline
Wang, ZJ; Pan, QY; Yang, LF; Zhou, H; Xu, CY; Yu, F; Wang, QS; Huang, S; He, JH; He, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201204, Peoples R China.
2016
发表期刊JOURNAL OF SYNCHROTRON RADIATION
ISSN1600-5775
卷号23页码:1323-1332
文章类型期刊论文
摘要X-ray diffraction is a common technique for determining crystal structures. The average time needed for the solution of a protein structure has been drastically reduced by a number of recent experimental and theoretical developments. Since high-throughput protein crystallography benefits from full automation of all steps that are carried out on a synchrotron beamline, an automatic crystal centring procedure is important for crystallographic beamlines. Fully automatic crystal alignment involves the application of optical methods to identify the crystal and move it onto the rotation axis and into the X-ray beam. Crystal recognition has complex dependencies on the illumination, crystal size and viewing angles due to effects such as local shading, inter-reflections and the presence of antifreezing elements. Here, a rapid procedure for crystal centring with multiple cameras using region segment thresholding is reported. Firstly, a simple illumination-invariant loop recognition and classification model is used by slicing a low-magnification loop image into small region segments, then classifying the loop into different types and aligning it to the beam position using feature vectors of the region segments. Secondly, an edge detection algorithm is used to find the crystal sample in a high-magnification image using region segment thresholding. Results show that this crystal centring method is extremely successful under fluctuating light states as well as for poorly frozen and opaque samples. Moreover, this crystal centring procedure is successfully integrated into the enhanced Blu-Ice data collection system at beamline BL17U1 at the Shanghai Synchrotron Radiation Facility as a routine method for an automatic crystal screening procedure.
关键词Crystal Centre Algorithm Recognition Crystallography Loop Centre
DOI10.1107/S160057751601451X
收录类别SCI
语种英语
WOS记录号WOS:000386928700006
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/26436
专题中科院上海应用物理研究所2011-2018年
通讯作者He, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201204, Peoples R China.
推荐引用方式
GB/T 7714
Wang, ZJ,Pan, QY,Yang, LF,et al. Automatic crystal centring procedure at the SSRF macromolecular crystallography beamline[J]. JOURNAL OF SYNCHROTRON RADIATION,2016,23:1323-1332.
APA Wang, ZJ.,Pan, QY.,Yang, LF.,Zhou, H.,Xu, CY.,...&He, JH .(2016).Automatic crystal centring procedure at the SSRF macromolecular crystallography beamline.JOURNAL OF SYNCHROTRON RADIATION,23,1323-1332.
MLA Wang, ZJ,et al."Automatic crystal centring procedure at the SSRF macromolecular crystallography beamline".JOURNAL OF SYNCHROTRON RADIATION 23(2016):1323-1332.
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