Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii
Wang, SS; Lv, JT; Ma, JY; Zhang, SZ; Zhang, SZ (reprint author), Chinese Acad Sci, Ecoenvironm Sci Res Ctr, 18 Shuangqing Rd, Beijing 100085, Peoples R China.
2016
发表期刊NANOTOXICOLOGY
ISSN1743-5390
卷号10期号:8页码:1129-1135
文章类型期刊论文
摘要It is necessary to elucidate cellular internalization and intracellular biotransformation in order to accurately assess the toxicity and fate of nanoparticles after interaction with organisms. Therefore, this work employed a combination of high resolution imaging and in situ detection spectroscopic techniques to systematically investigate the intracellular localization, morphology and chemical speciation of silver in the cells of Chlamydomonas reinhardtii, a unicellular freshwater green alga, after exposure to AgNPs coated with polyvinylpyrrolidone at a concentration of 2.0mg/L. High resolution secondary ion mass spectrometry and high-angle annular dark field scanning transmission electron microscopy together with energy dispersive spectroscopy and selected area electron diffraction collectively confirmed that after 48h of exposure, AgNPs entered the periplasmic space after cellular internalization into the algal cells. Silver was also found to coexist with sulfur inside the cytoplasm in both crystalline and amorphous forms, which were further identified as -Ag2S and silver thiolates with synchrotron X-ray absorption spectroscopy. In combination, these analyses demonstrated that silver inside algae could be attributed to the uptake and sequestration of Ag(+)ion released from AgNPs, which was further sequestrated into cellular compartments. This study provides solid evidence for particle internalization and biotransformation of AgNPs after interaction with algae.
关键词Chlamydomonas Reinhardtii Haadf-stem Nanosims Silver Nanoparticles Xas
DOI10.1080/17435390.2016.1179809
收录类别SCI
语种英语
WOS记录号WOS:000382337100011
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/26444
专题中科院上海应用物理研究所2011-2018年
通讯作者Zhang, SZ (reprint author), Chinese Acad Sci, Ecoenvironm Sci Res Ctr, 18 Shuangqing Rd, Beijing 100085, Peoples R China.
推荐引用方式
GB/T 7714
Wang, SS,Lv, JT,Ma, JY,et al. Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii[J]. NANOTOXICOLOGY,2016,10(8):1129-1135.
APA Wang, SS,Lv, JT,Ma, JY,Zhang, SZ,&Zhang, SZ .(2016).Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii.NANOTOXICOLOGY,10(8),1129-1135.
MLA Wang, SS,et al."Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii".NANOTOXICOLOGY 10.8(2016):1129-1135.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Cellular internaliza(894KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, SS]的文章
[Lv, JT]的文章
[Ma, JY]的文章
百度学术
百度学术中相似的文章
[Wang, SS]的文章
[Lv, JT]的文章
[Ma, JY]的文章
必应学术
必应学术中相似的文章
[Wang, SS]的文章
[Lv, JT]的文章
[Ma, JY]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。