Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii

doi:10.1080/17435390.2016.1179809

CAS OpenIR > 中科院上海应用物理研究所2011-2018年

	Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii
	Wang, SS; Lv, JT; Ma, JY; Zhang, SZ; Zhang, SZ (reprint author), Chinese Acad Sci, Ecoenvironm Sci Res Ctr, 18 Shuangqing Rd, Beijing 100085, Peoples R China.
	2016
发表期刊	NANOTOXICOLOGY
ISSN	1743-5390
卷号	10 期号:8 页码:1129-1135
文章类型	期刊论文
摘要	It is necessary to elucidate cellular internalization and intracellular biotransformation in order to accurately assess the toxicity and fate of nanoparticles after interaction with organisms. Therefore, this work employed a combination of high resolution imaging and in situ detection spectroscopic techniques to systematically investigate the intracellular localization, morphology and chemical speciation of silver in the cells of Chlamydomonas reinhardtii, a unicellular freshwater green alga, after exposure to AgNPs coated with polyvinylpyrrolidone at a concentration of 2.0mg/L. High resolution secondary ion mass spectrometry and high-angle annular dark field scanning transmission electron microscopy together with energy dispersive spectroscopy and selected area electron diffraction collectively confirmed that after 48h of exposure, AgNPs entered the periplasmic space after cellular internalization into the algal cells. Silver was also found to coexist with sulfur inside the cytoplasm in both crystalline and amorphous forms, which were further identified as -Ag2S and silver thiolates with synchrotron X-ray absorption spectroscopy. In combination, these analyses demonstrated that silver inside algae could be attributed to the uptake and sequestration of Ag(+)ion released from AgNPs, which was further sequestrated into cellular compartments. This study provides solid evidence for particle internalization and biotransformation of AgNPs after interaction with algae.
关键词	Chlamydomonas Reinhardtii Haadf-stem Nanosims Silver Nanoparticles Xas
DOI	10.1080/17435390.2016.1179809
收录类别	SCI
语种	英语
WOS记录号	WOS:000382337100011
引用统计
文献类型	期刊论文
条目标识符	http://ir.sinap.ac.cn/handle/331007/26444
专题	中科院上海应用物理研究所2011-2018年
通讯作者	Zhang, SZ (reprint author), Chinese Acad Sci, Ecoenvironm Sci Res Ctr, 18 Shuangqing Rd, Beijing 100085, Peoples R China.
推荐引用方式 GB/T 7714	Wang, SS,Lv, JT,Ma, JY,et al. Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii[J]. NANOTOXICOLOGY,2016,10(8):1129-1135.
APA	Wang, SS,Lv, JT,Ma, JY,Zhang, SZ,&Zhang, SZ .(2016).Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii.NANOTOXICOLOGY,10(8),1129-1135.
MLA	Wang, SS,et al."Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii".NANOTOXICOLOGY 10.8(2016):1129-1135.

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