CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii
Wang, SS; Lv, JT; Ma, JY; Zhang, SZ; Zhang, SZ (reprint author), Chinese Acad Sci, Ecoenvironm Sci Res Ctr, 18 Shuangqing Rd, Beijing 100085, Peoples R China.
2016
Source PublicationNANOTOXICOLOGY
ISSN1743-5390
Volume10Issue:8Pages:1129-1135
Subtype期刊论文
AbstractIt is necessary to elucidate cellular internalization and intracellular biotransformation in order to accurately assess the toxicity and fate of nanoparticles after interaction with organisms. Therefore, this work employed a combination of high resolution imaging and in situ detection spectroscopic techniques to systematically investigate the intracellular localization, morphology and chemical speciation of silver in the cells of Chlamydomonas reinhardtii, a unicellular freshwater green alga, after exposure to AgNPs coated with polyvinylpyrrolidone at a concentration of 2.0mg/L. High resolution secondary ion mass spectrometry and high-angle annular dark field scanning transmission electron microscopy together with energy dispersive spectroscopy and selected area electron diffraction collectively confirmed that after 48h of exposure, AgNPs entered the periplasmic space after cellular internalization into the algal cells. Silver was also found to coexist with sulfur inside the cytoplasm in both crystalline and amorphous forms, which were further identified as -Ag2S and silver thiolates with synchrotron X-ray absorption spectroscopy. In combination, these analyses demonstrated that silver inside algae could be attributed to the uptake and sequestration of Ag(+)ion released from AgNPs, which was further sequestrated into cellular compartments. This study provides solid evidence for particle internalization and biotransformation of AgNPs after interaction with algae.
KeywordChlamydomonas Reinhardtii Haadf-stem Nanosims Silver Nanoparticles Xas
DOI10.1080/17435390.2016.1179809
Indexed BySCI
Language英语
WOS IDWOS:000382337100011
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26444
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorZhang, SZ (reprint author), Chinese Acad Sci, Ecoenvironm Sci Res Ctr, 18 Shuangqing Rd, Beijing 100085, Peoples R China.
Recommended Citation
GB/T 7714
Wang, SS,Lv, JT,Ma, JY,et al. Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii[J]. NANOTOXICOLOGY,2016,10(8):1129-1135.
APA Wang, SS,Lv, JT,Ma, JY,Zhang, SZ,&Zhang, SZ .(2016).Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii.NANOTOXICOLOGY,10(8),1129-1135.
MLA Wang, SS,et al."Cellular internalization and intracellular biotransformation of silver nanoparticles in Chlamydomonas reinhardtii".NANOTOXICOLOGY 10.8(2016):1129-1135.
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