CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
CHIP involves in non-small cell lung cancer prognosis through VEGF pathway
Qian, TT; Wang, J; Wang, QF; Liu, YC; Yu, SJ; Wang, ZQ; Sun, DM; Wang, SL; Qian, TT (reprint author), Tongji Univ, Sch Life Sci & Technol, 1239 Siping Rd, Shanghai 200092, Peoples R China.
2016
Source PublicationBIOMEDICINE & PHARMACOTHERAPY
ISSN0753-3322
Volume83Pages:271-276
Subtype期刊论文
AbstractAim: CHIP (c-terminal Hsp70-interacting protein) is an E3 ligase playing vital roles in various cancers. The VEGF pathway has become an important therapeutic target in non-small cell lung cancer (NSCLC). However, little is known about the role of CHIP and the relationship between CHIP and VEGF-VEGFR2 (VEGF receptor 2) pathway in NSCLC. In this study we aimed to investigate the clinical function of CHIP in NSCLC and explore the relevant regulatory mechanism. Methods: QRT-PCR was performed to detect CHIP expression in NSCLC tissues. The association of CHIP expression and clinical parameters was analyzed using the Chi-square test. Kaplan-Meier and Cox analyses were performed to identify the role of CHIP in the prognosis of NSCLC patients. ELISA test was used to detect the VEGF secretion of NSCLC cells and western blot were used to detected the protein expression of VEGFR2 in NSCLC cells. Results: and the results revealed that CHIP expression was decreased in NSCLC tissues and significantly correlated with clinical stages, lymph node metastasis and distant metastasis (P < 0.05). Moreover, Kaplan-Meier and Cox regression analyses showed that patients with negative expression of CHIP had a shorter survival time and CHIP could be an independent prognostic biomarker. In addition, ELISA tests showed that CHIP negatively regulated the secretion level of VEGF. Furthermore, western blot assay indicated that the VEGFR2 protein level was reduced after CHIP over-expression. Conclusions: Taken together, our findings demonstrate for the first time that CHIP may serve as a promising prognostic biomarker for NSCLC patients and it may be involved in NSCLC angiogenesis through regulating VEGF secretion and expression of VEGFR2. (C) 2016 Published by Elsevier Masson SAS.
KeywordNsclc Chip Vegf-vegfr2 Prognosis
DOI10.1016/j.biopha.2016.06.015
Indexed BySCI
Language英语
WOS IDWOS:000390433400034
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26448
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorQian, TT (reprint author), Tongji Univ, Sch Life Sci & Technol, 1239 Siping Rd, Shanghai 200092, Peoples R China.
Recommended Citation
GB/T 7714
Qian, TT,Wang, J,Wang, QF,et al. CHIP involves in non-small cell lung cancer prognosis through VEGF pathway[J]. BIOMEDICINE & PHARMACOTHERAPY,2016,83:271-276.
APA Qian, TT.,Wang, J.,Wang, QF.,Liu, YC.,Yu, SJ.,...&Qian, TT .(2016).CHIP involves in non-small cell lung cancer prognosis through VEGF pathway.BIOMEDICINE & PHARMACOTHERAPY,83,271-276.
MLA Qian, TT,et al."CHIP involves in non-small cell lung cancer prognosis through VEGF pathway".BIOMEDICINE & PHARMACOTHERAPY 83(2016):271-276.
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