CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA
Chen, L; Cheng, DF; Liu, GZ; Dou, SP; Wang, YZ; Liu, XR; Liu, YX; Rusckowski, M; Rusckowski, M (reprint author), Univ Massachusetts, Div Nucl Med, Dept Radiol, Sch Med, 55 Lake Ave North, Worcester, MA 01655 USA.
2016
Source PublicationMOLECULAR IMAGING AND BIOLOGY
ISSN1536-1632
Volume18Issue:4Pages:527-534
Subtype期刊论文
AbstractPreviously, we demonstrated specific accumulation into bacteria of a 12-mer phosphorodiamidate morpholino (MORF) oligomer complementary to a ribosomal RNA (rRNA) segment found in all bacteria using the universal probe called Eub338 (Eub). Here, two MORF oligomers Eco and Kpn with sequences specific to the rRNA of Escherichia coli (Eco) and Klebsiella pneumoniae (Kpn) were investigated along with Eub and control (nonEub). To determine bacterial rRNA binding, oligomers were tagged with Alexa Fluor 633 (AF633) for fluorescence in situ hybridization (FISH) and fluorescence microscopy, and radiolabeled with technetium-99m (Tc-99m) for biodistribution and SPECT imaging in infected mice. By both FISH and fluorescence microscopy, Eub showed a positive signal in both E. coli and K. pneumoniae as expected, and Kpn showed significantly higher accumulation in K. pneumoniae with near background in E. coli (p < 0.01). Conversely, Eco was positive in both E. coli and K. pneumoniae, hence nonspecific. As determined by biodistribution, the accumulation of [Tc-99m]Kpn was higher in the thigh infected with live K. pneumoniae than with live E. coli (p = 0.05), and significantly higher than with heat-killed K. pneumoniae (p = 0.02) in the target thigh. By SPECT imaging, the accumulation of [Tc-99m]Kpn was obviously higher in its specific target of K. pneumoniae compared to an E. coli infected thigh. Kpn complementary to the rRNA of K. pneumoniae, labeled with Tc-99m or AF633, demonstrated specific binding to fixed and live K. pneumoniae in culture and in infected mice such that Tc-99m-labeled Kpn as the MORF oligomer may be useful for K. pneumoniae infection detection through imaging.
KeywordAntisense Morf Bacterial Ribosomal Rna Bacterial Infection Imaging
DOI10.1007/s11307-015-0927-4
Indexed BySCI
Language英语
WOS IDWOS:000379191800008
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26469
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorRusckowski, M (reprint author), Univ Massachusetts, Div Nucl Med, Dept Radiol, Sch Med, 55 Lake Ave North, Worcester, MA 01655 USA.
Recommended Citation
GB/T 7714
Chen, L,Cheng, DF,Liu, GZ,et al. Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA[J]. MOLECULAR IMAGING AND BIOLOGY,2016,18(4):527-534.
APA Chen, L.,Cheng, DF.,Liu, GZ.,Dou, SP.,Wang, YZ.,...&Rusckowski, M .(2016).Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA.MOLECULAR IMAGING AND BIOLOGY,18(4),527-534.
MLA Chen, L,et al."Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA".MOLECULAR IMAGING AND BIOLOGY 18.4(2016):527-534.
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