Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA
Chen, L; Cheng, DF; Liu, GZ; Dou, SP; Wang, YZ; Liu, XR; Liu, YX; Rusckowski, M; Rusckowski, M (reprint author), Univ Massachusetts, Div Nucl Med, Dept Radiol, Sch Med, 55 Lake Ave North, Worcester, MA 01655 USA.
2016
发表期刊MOLECULAR IMAGING AND BIOLOGY
ISSN1536-1632
卷号18期号:4页码:527-534
文章类型期刊论文
摘要Previously, we demonstrated specific accumulation into bacteria of a 12-mer phosphorodiamidate morpholino (MORF) oligomer complementary to a ribosomal RNA (rRNA) segment found in all bacteria using the universal probe called Eub338 (Eub). Here, two MORF oligomers Eco and Kpn with sequences specific to the rRNA of Escherichia coli (Eco) and Klebsiella pneumoniae (Kpn) were investigated along with Eub and control (nonEub). To determine bacterial rRNA binding, oligomers were tagged with Alexa Fluor 633 (AF633) for fluorescence in situ hybridization (FISH) and fluorescence microscopy, and radiolabeled with technetium-99m (Tc-99m) for biodistribution and SPECT imaging in infected mice. By both FISH and fluorescence microscopy, Eub showed a positive signal in both E. coli and K. pneumoniae as expected, and Kpn showed significantly higher accumulation in K. pneumoniae with near background in E. coli (p < 0.01). Conversely, Eco was positive in both E. coli and K. pneumoniae, hence nonspecific. As determined by biodistribution, the accumulation of [Tc-99m]Kpn was higher in the thigh infected with live K. pneumoniae than with live E. coli (p = 0.05), and significantly higher than with heat-killed K. pneumoniae (p = 0.02) in the target thigh. By SPECT imaging, the accumulation of [Tc-99m]Kpn was obviously higher in its specific target of K. pneumoniae compared to an E. coli infected thigh. Kpn complementary to the rRNA of K. pneumoniae, labeled with Tc-99m or AF633, demonstrated specific binding to fixed and live K. pneumoniae in culture and in infected mice such that Tc-99m-labeled Kpn as the MORF oligomer may be useful for K. pneumoniae infection detection through imaging.
关键词Antisense Morf Bacterial Ribosomal Rna Bacterial Infection Imaging
DOI10.1007/s11307-015-0927-4
收录类别SCI
语种英语
WOS记录号WOS:000379191800008
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/26469
专题中科院上海应用物理研究所2011-2018年
通讯作者Rusckowski, M (reprint author), Univ Massachusetts, Div Nucl Med, Dept Radiol, Sch Med, 55 Lake Ave North, Worcester, MA 01655 USA.
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Chen, L,Cheng, DF,Liu, GZ,et al. Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA[J]. MOLECULAR IMAGING AND BIOLOGY,2016,18(4):527-534.
APA Chen, L.,Cheng, DF.,Liu, GZ.,Dou, SP.,Wang, YZ.,...&Rusckowski, M .(2016).Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA.MOLECULAR IMAGING AND BIOLOGY,18(4),527-534.
MLA Chen, L,et al."Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA".MOLECULAR IMAGING AND BIOLOGY 18.4(2016):527-534.
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