Zuo, XL (reprint author), Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China.
; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr, Shanghai 201800, Peoples R China.
文章类型:
期刊论文
英文摘要:
The fixed dynamic :range of traditional biosensors limits their utility in several real applications. For example, viral load monitoring requires the dynamic range spans Several orders of magnitude; whereas, monitoring of drugs requires extremely narrow dynamic range, To overcome this limitation; here, we devised tunable biosensing interface using allosteric DNA tetrahedral bioprobes to tune the dynamic range of DNA biosensors. Our strategy takes the advantage of the readily and flexible structure design, and predictable geometric Tecorifiguration of DNA nanotechtiology. We reconfigured the-DNA tetrahedral bioprobes by inserting the effector `sequence into the. DNA tetrahedron, through which, the binding affinity:of DNA tetrahedral bioprobes can be tuned. Asa result, the detection limit of DNA biosensors can be prograrrunably regulated. The,dynamic range of DNA biosensors can be tuned (narrowed: or extended) for up to 100-fold. Using the regulation of binding affinity, we realized the; capture and release of biomaecules by tuning the binding behavior of DNA tetrahedral bioprobes.
