CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Electrochemical detection of PCR amplicons of Escherichia coli genome based on DNA nanostructural probes and polyHRP enzyme
Wen, YL; Wang, LL; Xu, L; Li, LY; Ren, SZ; Cao, CM; Jia, NQ; Aldalbahi, A; Song, SP; Shi, JY; Xia, JY; Liu, G; Zuo, XL; Liu, G (reprint author), Shanghai Inst Measurement & Testing Technol, Div Chem & Ionizing Radiat Measurement Technol, Shanghai 201203, Peoples R China.; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.; Aldalbahi, A (reprint author), King Saud Univ, Dept Chem, Riyadh 11451, Saudi Arabia.
2016
Source PublicationANALYST
ISSN0003-2654
Volume141Issue:18Pages:5304-5310
Subtype期刊论文
AbstractFast, portable and sensitive analysis of E. coli is becoming an important challenge in many critical fields (e.g., food safety, environmental monitoring and clinical diagnosis). Thus, electrochemical biosensing of PCR amplicons from the bacterial genome has attracted reasonable research attention. In this work, we utilized a 3D DNA tetrahedral probe to establish a "sandwich-type" electrochemical DNA biosensor for sensitive and specific analysis of a 250 bp unpurified PCR amplicon from the uidA gene of the E. coli genome. Asymmetric PCR was used to produce single-stranded PCR products. Streptavidin-polyHRP80 was employed to improve the signal gain during electrochemical detection. We optimized important experimental conditions for DNA sensing, including the streptavidin-polyHRP, the signal probe and the ion strength. Finally, we achieved a remarkable sensitivity of 10 fM synthetic DNA target, and successfully performed the analysis of PCR amplicons from as low as 0.2 pg mu L-1 of E. coli genome. Compared with traditional single stranded DNA (ssDNA) probe based detection, our present work demonstrated 3 orders of magnitude improvement in sensitivity. In addition, our electrochemical DNA biosensor was 4 orders of magnitude more sensitive than normal electrophoretic analysis of PCR products. Our work made important progress in DNA nanostructured probe-based biosensors toward application in real applications.
DOI10.1039/c6an01435f
Indexed BySCI
Language英语
WOS IDWOS:000382698300012
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26493
Collection中科院上海应用物理研究所2011-2019年
Corresponding AuthorLiu, G (reprint author), Shanghai Inst Measurement & Testing Technol, Div Chem & Ionizing Radiat Measurement Technol, Shanghai 201203, Peoples R China.; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China.; Aldalbahi, A (reprint author), King Saud Univ, Dept Chem, Riyadh 11451, Saudi Arabia.
Recommended Citation
GB/T 7714
Wen, YL,Wang, LL,Xu, L,et al. Electrochemical detection of PCR amplicons of Escherichia coli genome based on DNA nanostructural probes and polyHRP enzyme[J]. ANALYST,2016,141(18):5304-5310.
APA Wen, YL.,Wang, LL.,Xu, L.,Li, LY.,Ren, SZ.,...&Aldalbahi, A .(2016).Electrochemical detection of PCR amplicons of Escherichia coli genome based on DNA nanostructural probes and polyHRP enzyme.ANALYST,141(18),5304-5310.
MLA Wen, YL,et al."Electrochemical detection of PCR amplicons of Escherichia coli genome based on DNA nanostructural probes and polyHRP enzyme".ANALYST 141.18(2016):5304-5310.
Files in This Item: Download All
File Name/Size DocType Version Access License
Electrochemical dete(4360KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wen, YL]'s Articles
[Wang, LL]'s Articles
[Xu, L]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wen, YL]'s Articles
[Wang, LL]'s Articles
[Xu, L]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wen, YL]'s Articles
[Wang, LL]'s Articles
[Xu, L]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Electrochemical detection of PCR amplicons of Escherichia coli genome based on DNA nanostructural probes and polyHRP enzyme.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.