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题名: High Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid
作者: Zhao, ZJ; Zhang, P; Yang, JX; Hao, CC; Zhou, XF; Li, B
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
出版日期: 2016
卷号: 44, 期号:8, 页码:1215-1220
关键词: Atomic force microscopy ; Single-molecule ; High-resolution imaging ; Immunoglobulin G ; DNA origami
DOI: 10.11895/j.issn.0253.3820.160143
通讯作者: Li, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,Key Lab Interfa, Shanghai 201800, Peoples R China. ; Li, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,Key Lab Interfa, Shanghai 201800, Peoples R China.
文章类型: 期刊论文
英文摘要: Atomic force microscopy (AFM) is one of important tools for studying the structure and function of biomolecules due to its nanoscale capability. A well prepared sample was the primary key issue for high. resolution imaging macromolecules. Here, by using self. assembly technology of DNA origami, antigens were modified on DNA origami. Afterward, antibodies reacted specifically with antigens by a molecular recognition way. Then, a nanostructure formed from DNA origami and antigen. antibody complexes. By the aid of adsorption character of DNA origami, individual antibodies could selectively adsorb on mica surface. As a result, the ultrastructural morphology image of a single IgG molecule, the characteristic "Y. shaped. domains, was resolved in liquid. The way for adsorbing biomolecules on the mica surface in a liquid environment was simple and convenient, and it may be useful to detect and measure biomoleculers at the single-molecule level with AFM.
收录类别: SCI
语种: 中文
WOS记录号: WOS:000389035300007
ISSN号: 0253-3820
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/26528
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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