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High Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid
Zhao, ZJ; Zhang, P; Yang, JX; Hao, CC; Zhou, XF; Li, B; Li, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,Key Lab Interfa, Shanghai 201800, Peoples R China.; Li, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,Key Lab Interfa, Shanghai 201800, Peoples R China.
2016
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume44Issue:8Pages:1215-1220
Subtype期刊论文
AbstractAtomic force microscopy (AFM) is one of important tools for studying the structure and function of biomolecules due to its nanoscale capability. A well prepared sample was the primary key issue for high. resolution imaging macromolecules. Here, by using self. assembly technology of DNA origami, antigens were modified on DNA origami. Afterward, antibodies reacted specifically with antigens by a molecular recognition way. Then, a nanostructure formed from DNA origami and antigen. antibody complexes. By the aid of adsorption character of DNA origami, individual antibodies could selectively adsorb on mica surface. As a result, the ultrastructural morphology image of a single IgG molecule, the characteristic "Y. shaped. domains, was resolved in liquid. The way for adsorbing biomolecules on the mica surface in a liquid environment was simple and convenient, and it may be useful to detect and measure biomoleculers at the single-molecule level with AFM.
KeywordAtomic Force Microscopy Single-molecule High-resolution Imaging Immunoglobulin g Dna Origami
DOI10.11895/j.issn.0253.3820.160143
Indexed BySCI
Language中文
WOS IDWOS:000389035300007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26528
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorLi, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,Key Lab Interfa, Shanghai 201800, Peoples R China.; Li, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,Key Lab Interfa, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Zhao, ZJ,Zhang, P,Yang, JX,et al. High Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2016,44(8):1215-1220.
APA Zhao, ZJ.,Zhang, P.,Yang, JX.,Hao, CC.,Zhou, XF.,...&Li, B .(2016).High Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,44(8),1215-1220.
MLA Zhao, ZJ,et al."High Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 44.8(2016):1215-1220.
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