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Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device
Zhang, Y; Li, Q; Guo, LJ; Huang, Q; Shi, JY; Yang, Y; Liu, DS; Fan, CH; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.; Liu, DS (reprint author), Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China.
2016
Source PublicationANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN1433-7851
Volume55Issue:40Pages:12450-12454
Subtype期刊论文
AbstractThe polymerase chain reaction (PCR) is a powerful method for exponentially amplifying very low amounts of target DNA from genetic, clinical, and forensic samples. However, the heating and cooling steps in PCR largely hamper the miniaturization of thermocyclers for on-site detection of pathogens and point-of-care tests. Herein, we devise an ion-mediated PCR (IM-PCR) strategy by exploiting ion-induced DNA denaturation/renaturation cycles. DNA duplexes are effectively denatured in alkaline solutions; whereas, the denatured single-stranded DNA strands readily reform duplexes at neutral pH. By using an integrated micro-chip that can programmably control the solution pH simply switching the potential in a range of several hundred millivolts, we can trigger IM-PCR at a constant temperature. Analogously to thermal cycling, 30 cycles of pH-induced denaturation/renaturation were used to amplify protein DNA fragments as confirmed by DNA sequencing. We anticipate that this portable, low-cost, and scalable IM-PCR holds great promise for widespread biological, clinical, and environmental applications.
KeywordDna Amplification Isothermal Pcr Microfluidic Devices Ph Cycling Ph-stats
DOI10.1002/anie.201606137
Indexed BySCI
Language英语
WOS IDWOS:000384713700057
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26551
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorFan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.; Liu, DS (reprint author), Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China.
Recommended Citation
GB/T 7714
Zhang, Y,Li, Q,Guo, LJ,et al. Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2016,55(40):12450-12454.
APA Zhang, Y.,Li, Q.,Guo, LJ.,Huang, Q.,Shi, JY.,...&Liu, DS .(2016).Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,55(40),12450-12454.
MLA Zhang, Y,et al."Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 55.40(2016):12450-12454.
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