Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device
Zhang, Y; Li, Q; Guo, LJ; Huang, Q; Shi, JY; Yang, Y; Liu, DS; Fan, CH; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.; Liu, DS (reprint author), Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China.
2016
发表期刊ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN1433-7851
卷号55期号:40页码:12450-12454
文章类型期刊论文
摘要The polymerase chain reaction (PCR) is a powerful method for exponentially amplifying very low amounts of target DNA from genetic, clinical, and forensic samples. However, the heating and cooling steps in PCR largely hamper the miniaturization of thermocyclers for on-site detection of pathogens and point-of-care tests. Herein, we devise an ion-mediated PCR (IM-PCR) strategy by exploiting ion-induced DNA denaturation/renaturation cycles. DNA duplexes are effectively denatured in alkaline solutions; whereas, the denatured single-stranded DNA strands readily reform duplexes at neutral pH. By using an integrated micro-chip that can programmably control the solution pH simply switching the potential in a range of several hundred millivolts, we can trigger IM-PCR at a constant temperature. Analogously to thermal cycling, 30 cycles of pH-induced denaturation/renaturation were used to amplify protein DNA fragments as confirmed by DNA sequencing. We anticipate that this portable, low-cost, and scalable IM-PCR holds great promise for widespread biological, clinical, and environmental applications.
关键词Dna Amplification Isothermal Pcr Microfluidic Devices Ph Cycling Ph-stats
DOI10.1002/anie.201606137
收录类别SCI
语种英语
WOS记录号WOS:000384713700057
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/26551
专题中科院上海应用物理研究所2011-2018年
通讯作者Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China.; Liu, DS (reprint author), Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China.
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GB/T 7714
Zhang, Y,Li, Q,Guo, LJ,et al. Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2016,55(40):12450-12454.
APA Zhang, Y.,Li, Q.,Guo, LJ.,Huang, Q.,Shi, JY.,...&Liu, DS .(2016).Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,55(40),12450-12454.
MLA Zhang, Y,et al."Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 55.40(2016):12450-12454.
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