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CAS OpenIR  > 中科院上海应用物理研究所2011-2017年  > 期刊论文
题名:
Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device
作者: Zhang, Y; Li, Q; Guo, LJ; Huang, Q; Shi, JY; Yang, Y; Liu, DS; Fan, CH
刊名: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
出版日期: 2016
卷号: 55, 期号:40, 页码:12450-12454
关键词: dna amplification ; isothermal PCR ; microfluidic devices ; pH cycling ; pH-stats
DOI: 10.1002/anie.201606137
通讯作者: Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China. ; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China. ; Liu, DS (reprint author), Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China.
文章类型: 期刊论文
英文摘要: The polymerase chain reaction (PCR) is a powerful method for exponentially amplifying very low amounts of target DNA from genetic, clinical, and forensic samples. However, the heating and cooling steps in PCR largely hamper the miniaturization of thermocyclers for on-site detection of pathogens and point-of-care tests. Herein, we devise an ion-mediated PCR (IM-PCR) strategy by exploiting ion-induced DNA denaturation/renaturation cycles. DNA duplexes are effectively denatured in alkaline solutions; whereas, the denatured single-stranded DNA strands readily reform duplexes at neutral pH. By using an integrated micro-chip that can programmably control the solution pH simply switching the potential in a range of several hundred millivolts, we can trigger IM-PCR at a constant temperature. Analogously to thermal cycling, 30 cycles of pH-induced denaturation/renaturation were used to amplify protein DNA fragments as confirmed by DNA sequencing. We anticipate that this portable, low-cost, and scalable IM-PCR holds great promise for widespread biological, clinical, and environmental applications.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000384713700057
ISSN号: 1433-7851
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/26551
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Zhang, Y,Li, Q,Guo, LJ,et al. Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2016-01-01,55(40):12450-12454.
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文件名: Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device.pdf
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