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题名:
Magnetic Fe3O4 nanoparticle catalyzed chemiluminescence for detection of nitric oxide in living cells
作者: Wang, HL; Li, M; Wang, B; Wang, M; Kurash, I; Zhang, XZ; Feng, WY
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期: 2016
卷号: 408, 期号:20, 页码:5479-5488
关键词: Fe3O4 nanoparticle ; Rapid capture ; Intracellular nitric oxide detection
DOI: 10.1007/s00216-016-9646-1
通讯作者: Li, M (reprint author), Qilu Univ Technol, Sch Mat Sci & Engn, 3501 Daxue Rd, Jinan 250353, Shandong, Peoples R China. ; Wang, B (reprint author), Chinese Acad Sci, Inst High Energy Phys, Lab Biomed Effects Nanomat & Nanosafety, 19B Yu Quan Rd, Beijing 100049, Peoples R China.
文章类型: 期刊论文
英文摘要: Direct and real-time measurement of nitric oxide (NO) in biological media is very difficult due to its transient nature. Fe3O4 nanoparticles (nanoFe(3)O(4)) because of their unique catalytic activities have attracted much attention as catalysts in a variety of organic and inorganic reactions. In this work, we have developed a magnetic Fe3O4 nanoparticle-based rapid-capture system for real-time detection of cellular NO. The basic principle is that the nanoFe(3)O(4) can catalyze the decomposition of H2O2 in the system to generate superoxide anion (O-2 (center dot-)) and the O-2 (center dot-) can serve as an effective NO center dot trapping agent yielding peroxynitrite oxide anion, ONOO-. Then the concentration of NO in cells can be facilely determined via peroxynitrite-induced luminol chemiluminescence. The linear range of the method is from 10(-4) to 10(-8) mol/L, and the detection of limit (3 sigma, n = 11) is as low as 3.16 x 10(-9) mol/L. By using this method, the NO concentration in 0.1 and 0.5 mg/L LPS-stimulated BV2 cells was measured as 4.9 and 11.3 mu M, respectively. Surface measurements by synchrotron X-ray photoelectron spectroscopy (SRXPS) and scanning transmission X-ray microscopy (STXM) demonstrate the catalytic mechanism of the nanoFe(3)O(4)-based system is that the significantly excess Fe(II) exists on the surface of nanoFe(3)O(4) and mediates the rapid heterogeneous electron transfer, thus presenting a new Fe2O3 phase on the surface.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000379624400009
ISSN号: 1618-2642
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/26567
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Wang, HL,Li, M,Wang, B,et al. Magnetic Fe3O4 nanoparticle catalyzed chemiluminescence for detection of nitric oxide in living cells[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016-01-01,408(20):5479-5488.
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