Yan, JH (reprint author), Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China.
; Yan, JH
; Gao, GF (reprint author), Shenzhen Third Peoples Hosp, Shenzhen Key Lab Pathogen & Immun, Shenzhen 518112, Peoples R China.
; Yan, JH (reprint author), Anhui Univ, Sch Life Sci, Hefei 230039, Peoples R China.
; Gao, GF (reprint author), Chinese Acad Sci, Beijing Inst Life Sci, Res Network Immun & Hlth, Beijing 100101, Peoples R China.
; Yan, JH
; Gao, GF (reprint author), Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China.
; Gao, GF (reprint author), Chinese Acad Sci, CAS Ctr Influenza Res & Early Warning, Beijing 100101, Peoples R China.
; Gao, GF (reprint author), Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China.
; Gao, GF (reprint author), Chinese Ctr Dis Control & Prevent China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China.
; Yan, JH (reprint author), Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China.
; Gao, GF (reprint author), Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310003, Zhejiang, Peoples R China.
; Gao, GF (reprint author), Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China.
文章类型:
期刊论文
英文摘要:
The 2015-2016 outbreak of Zika virus (ZIKV) disease has affected many countries and is a major public health concern. ZIKV is associated with fetal microcephaly and neurological complications, and countermeasures are needed to treat and prevent ZIKV infection. We report the isolation of 13 specific human monoclonal antibodies from a single patient infected with ZIKV. Two of the isolated antibodies (Z23 and Z3L1) demonstrated potent ZIKV-specific neutralization in vitro without binding or neutralizing activity against strains 1 to 4 of dengue virus, the closest relative to ZIKV. These two antibodies provided postexposure protection to mice in vivo. Structural studies revealed that Z23 and Z3L1 bound to tertiary epitopes in envelope protein domain I, II, or III, indicating potential targets for ZIKV-specific therapy. Our results suggest the potential of antibody-based therapeutics and provide a structure-based rationale for the design of future ZIKV-specific vaccines.
Wang, QH,Yang, HB,Liu, XQ,et al. Molecular determinants of human neutralizing antibodies isolated from a patient infected with Zika virus[J]. SCIENCE TRANSLATIONAL MEDICINE,2016-01-01,8(369):-.
