PolyA-Mediated DNA Assembly on Gold Nanoparticles for Thermodynamically Favorable and Rapid Hybridization Analysis

doi:10.1021/acs.analchem.6b00891

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	PolyA-Mediated DNA Assembly on Gold Nanoparticles for Thermodynamically Favorable and Rapid Hybridization Analysis
	Zhu, D; Song, P; Shen, JW; Su, S; Chao, J; Aldalbahi, A; Zhou, Z; Song, SP; Fan, CH; Zuo, XL; Tian, Y; Wang, LH; Pei, H; Fan, CH (reprint author), Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Pei, H (reprint author), East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China.
	2016
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
卷号	88 期号:9 页码:4949-4954
文章类型	期刊论文
摘要	Understanding the behavior of biomolecules on nanointerface is critical in bioanalysis, which is great challenge due to the instability and the difficulty to control the orientation and loading density of biomolecules. Here, we investigated the thermodynamics and kinetics of DNA hybridization on gold nanoparticle, with the aim to improve the efficiency and speed of DNA analysis. We achieved precise and quantitative surface control by applying a recently developed poly adenines (polyA)-based assembly strategy on gold nanopartides (DNA-AuNPs). PolyA served as an effective anchoring block based on the preferential binding with the AuNP surface and the appended recognition block adopted an upright conformation that favors DNA hybridization. The lateral spacing and surface density of DNA on AuNPs can be systematically modulated by adjusting the length of polyA block. We found the stability of duplex on AuNP was enhanced with the increasing length of polyA block. When the length of polyA block reached to 40 bases, the thermodynamic properties were more similar to that of duplex in solution. Fast hybridization rate was observed on the cliblock DNA-AuNPs and was increased along with the length of polyA block. We consider the high stability and excellent hybridization performance come from the minimization of the DNA DNA and DNA-AuNP interactions with the use of polyA block. This study provides better understanding of the behavior of biomolecules on the nanointerface and opens new opportunities to construct high-efficiency and high-speed biosensors for DNA analysis.
DOI	10.1021/acs.analchem.6b00891
收录类别	SCI
语种	英语
WOS记录号	WOS:000379636600050
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.sinap.ac.cn/handle/331007/26597
专题	中科院上海应用物理研究所2011-2018年
通讯作者	Fan, CH (reprint author), Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China.; Fan, CH (reprint author), Chinese Acad Sci, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Pei, H (reprint author), East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China.
推荐引用方式 GB/T 7714	Zhu, D,Song, P,Shen, JW,et al. PolyA-Mediated DNA Assembly on Gold Nanoparticles for Thermodynamically Favorable and Rapid Hybridization Analysis[J]. ANALYTICAL CHEMISTRY,2016,88(9):4949-4954.
APA	Zhu, D.,Song, P.,Shen, JW.,Su, S.,Chao, J.,...&Pei, H .(2016).PolyA-Mediated DNA Assembly on Gold Nanoparticles for Thermodynamically Favorable and Rapid Hybridization Analysis.ANALYTICAL CHEMISTRY,88(9),4949-4954.
MLA	Zhu, D,et al."PolyA-Mediated DNA Assembly on Gold Nanoparticles for Thermodynamically Favorable and Rapid Hybridization Analysis".ANALYTICAL CHEMISTRY 88.9(2016):4949-4954.

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