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题名:
Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope
作者: Wang, SP; Deng, SH; Cai, XQ; Hou, SG; Li, JJ; Gao, ZS; Li, J; Wang, LH; Fan, CH
刊名: SCIENCE CHINA-CHEMISTRY
出版日期: 2016
卷号: 59, 期号:11, 页码:1519-1524
关键词: telomere ; superresolution imaging ; stimulated emission depletion (STED) microscopy ; fluorescence in situ hybridization (FISH)
DOI: 10.1007/s11426-016-0020-9
通讯作者: Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Div Phys Biol,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China. ; Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
文章类型: 期刊论文
英文摘要: The significant role of telomeres in cells has attracted much attention since they were discovered. Fluorescence imaging is an effective method to study subcellular structures like telomeres. However, the diffraction limit of traditional optical microscope hampers further investigation on them. Recent progress on superresolution fluorescence microscopy has broken this limit. In this work, we used stimulated emission depletion (STED) microscope to observe fluorescence-labeled telomeres in interphase cell nuclei. The results showed that the size of fluorescent puncta representing telomeres under the STED microscope was much smaller than that under the confocal microscope. Two adjacent telomeres were clearly separated via STED imaging, which could hardly be discriminated by confocal microscopy due to the diffraction limit. We conclude that STED microscope is a more powerful tool that enable us to obtain detailed information about telomeres.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000387571100021
ISSN号: 1674-7291
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/26640
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Wang, SP,Deng, SH,Cai, XQ,et al. Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope[J]. SCIENCE CHINA-CHEMISTRY,2016-01-01,59(11):1519-1524.
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文件名: Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope.pdf
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