CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope
Wang, SP; Deng, SH; Cai, XQ; Hou, SG; Li, JJ; Gao, ZS; Li, J; Wang, LH; Fan, CH; Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Div Phys Biol,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
2016
Source PublicationSCIENCE CHINA-CHEMISTRY
ISSN1674-7291
Volume59Issue:11Pages:1519-1524
Subtype期刊论文
AbstractThe significant role of telomeres in cells has attracted much attention since they were discovered. Fluorescence imaging is an effective method to study subcellular structures like telomeres. However, the diffraction limit of traditional optical microscope hampers further investigation on them. Recent progress on superresolution fluorescence microscopy has broken this limit. In this work, we used stimulated emission depletion (STED) microscope to observe fluorescence-labeled telomeres in interphase cell nuclei. The results showed that the size of fluorescent puncta representing telomeres under the STED microscope was much smaller than that under the confocal microscope. Two adjacent telomeres were clearly separated via STED imaging, which could hardly be discriminated by confocal microscopy due to the diffraction limit. We conclude that STED microscope is a more powerful tool that enable us to obtain detailed information about telomeres.
KeywordTelomere Superresolution Imaging Stimulated Emission Depletion (Sted) Microscopy Fluorescence In Situ Hybridization (Fish)
DOI10.1007/s11426-016-0020-9
Indexed BySCI
Language英语
WOS IDWOS:000387571100021
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26640
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorLi, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Div Phys Biol,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Wang, SP,Deng, SH,Cai, XQ,et al. Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope[J]. SCIENCE CHINA-CHEMISTRY,2016,59(11):1519-1524.
APA Wang, SP.,Deng, SH.,Cai, XQ.,Hou, SG.,Li, JJ.,...&Li, J .(2016).Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope.SCIENCE CHINA-CHEMISTRY,59(11),1519-1524.
MLA Wang, SP,et al."Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope".SCIENCE CHINA-CHEMISTRY 59.11(2016):1519-1524.
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