Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope
Wang, SP; Deng, SH; Cai, XQ; Hou, SG; Li, JJ; Gao, ZS; Li, J; Wang, LH; Fan, CH; Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Div Phys Biol,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
2016
发表期刊SCIENCE CHINA-CHEMISTRY
ISSN1674-7291
卷号59期号:11页码:1519-1524
文章类型期刊论文
摘要The significant role of telomeres in cells has attracted much attention since they were discovered. Fluorescence imaging is an effective method to study subcellular structures like telomeres. However, the diffraction limit of traditional optical microscope hampers further investigation on them. Recent progress on superresolution fluorescence microscopy has broken this limit. In this work, we used stimulated emission depletion (STED) microscope to observe fluorescence-labeled telomeres in interphase cell nuclei. The results showed that the size of fluorescent puncta representing telomeres under the STED microscope was much smaller than that under the confocal microscope. Two adjacent telomeres were clearly separated via STED imaging, which could hardly be discriminated by confocal microscopy due to the diffraction limit. We conclude that STED microscope is a more powerful tool that enable us to obtain detailed information about telomeres.
关键词Telomere Superresolution Imaging Stimulated Emission Depletion (Sted) Microscopy Fluorescence In Situ Hybridization (Fish)
DOI10.1007/s11426-016-0020-9
收录类别SCI
语种英语
WOS记录号WOS:000387571100021
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/26640
专题中科院上海应用物理研究所2011-2018年
通讯作者Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Div Phys Biol,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
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Wang, SP,Deng, SH,Cai, XQ,et al. Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope[J]. SCIENCE CHINA-CHEMISTRY,2016,59(11):1519-1524.
APA Wang, SP.,Deng, SH.,Cai, XQ.,Hou, SG.,Li, JJ.,...&Li, J .(2016).Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope.SCIENCE CHINA-CHEMISTRY,59(11),1519-1524.
MLA Wang, SP,et al."Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope".SCIENCE CHINA-CHEMISTRY 59.11(2016):1519-1524.
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