Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu2+ exposure
Hu, XJ; Liu, ZX; Wang, YD; Li, XL; Hu, J; Lu, JH; Lu, JH (reprint author), Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Lu, JH (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
2016
发表期刊NUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042
卷号27期号:3页码:-
文章类型期刊论文
摘要Copper ions (e.g., Cu2+) have outstanding antibacterial properties, but the exact mechanism is rather complex and not fully understood. In this work, synchrotron Fourier transform infrared (FTIR) spectroscopy was used as an analytical tool to investigate the CuCl2-induced biochemical changes in Escherichia coli. Our spectral measurements indicated that this technique is sensitive enough to detect changes in membrane lipids, nucleic acids, peptidoglycans and proteins of Cu2+-treated bacteria. Interestingly, for short-time treated cells, the effects on phospholipid composition were clearly shown, while no significant alterations of proteins, nucleic acids and peptidoglycans were found. PeakForce quantitative nano-mechanics mode atomic force microscopy (AFM) confirmed the changes in the topography and mechanical properties of bacteria upon the Cu2+ exposure. This study demonstrated that FTIR spectroscopy combined with AFM can provide more comprehensive evaluation on the biochemical and mechanical responses of bacteria to copper.
关键词Copper Ions Antibacterial Effect Escherichia Coli Synchrotron Ftir Spectroscopy Atomic Force Microscopy
DOI10.1007/s41365-016-0067-9
收录类别SCI
语种英语
WOS记录号WOS:000380038900007
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/26643
专题中科院上海应用物理研究所2011-2018年
通讯作者Lu, JH (reprint author), Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Lu, JH (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
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GB/T 7714
Hu, XJ,Liu, ZX,Wang, YD,et al. Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu2+ exposure[J]. NUCLEAR SCIENCE AND TECHNIQUES,2016,27(3):-.
APA Hu, XJ.,Liu, ZX.,Wang, YD.,Li, XL.,Hu, J.,...&Lu, JH .(2016).Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu2+ exposure.NUCLEAR SCIENCE AND TECHNIQUES,27(3),-.
MLA Hu, XJ,et al."Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu2+ exposure".NUCLEAR SCIENCE AND TECHNIQUES 27.3(2016):-.
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