CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu2+ exposure
Hu, XJ; Liu, ZX; Wang, YD; Li, XL; Hu, J; Lu, JH; Lu, JH (reprint author), Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Lu, JH (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
2016
Source PublicationNUCLEAR SCIENCE AND TECHNIQUES
ISSN1001-8042
Volume27Issue:3Pages:-
Subtype期刊论文
AbstractCopper ions (e.g., Cu2+) have outstanding antibacterial properties, but the exact mechanism is rather complex and not fully understood. In this work, synchrotron Fourier transform infrared (FTIR) spectroscopy was used as an analytical tool to investigate the CuCl2-induced biochemical changes in Escherichia coli. Our spectral measurements indicated that this technique is sensitive enough to detect changes in membrane lipids, nucleic acids, peptidoglycans and proteins of Cu2+-treated bacteria. Interestingly, for short-time treated cells, the effects on phospholipid composition were clearly shown, while no significant alterations of proteins, nucleic acids and peptidoglycans were found. PeakForce quantitative nano-mechanics mode atomic force microscopy (AFM) confirmed the changes in the topography and mechanical properties of bacteria upon the Cu2+ exposure. This study demonstrated that FTIR spectroscopy combined with AFM can provide more comprehensive evaluation on the biochemical and mechanical responses of bacteria to copper.
KeywordCopper Ions Antibacterial Effect Escherichia Coli Synchrotron Ftir Spectroscopy Atomic Force Microscopy
DOI10.1007/s41365-016-0067-9
Indexed BySCI
Language英语
WOS IDWOS:000380038900007
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26643
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorLu, JH (reprint author), Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.; Lu, JH (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
Recommended Citation
GB/T 7714
Hu, XJ,Liu, ZX,Wang, YD,et al. Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu2+ exposure[J]. NUCLEAR SCIENCE AND TECHNIQUES,2016,27(3):-.
APA Hu, XJ.,Liu, ZX.,Wang, YD.,Li, XL.,Hu, J.,...&Lu, JH .(2016).Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu2+ exposure.NUCLEAR SCIENCE AND TECHNIQUES,27(3),-.
MLA Hu, XJ,et al."Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu2+ exposure".NUCLEAR SCIENCE AND TECHNIQUES 27.3(2016):-.
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