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Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode | |
Wang, RH; Liu, Q; Wu, ZL; Wang, ML; Chen, XQ; Chen, XQ (reprint author), Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China. | |
2016 | |
Source Publication | JOURNAL OF SEPARATION SCIENCE
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ISSN | 1615-9306 |
Volume | 39Issue:20Pages:3983-3989 |
Subtype | 期刊论文 |
Abstract | An off-line two-dimensional high-speed counter-current chromatography strategy combined with the wavelength switching technique and extrusion elution mode was successfully developed and applied to the isolation of polar antioxidants from Abelmoschus esculentus (L). Moench. Target-guided by the result of 2,2-diphenyl-1-picrylhydrazyl screening assay, four antioxidants were obtained with purities over 90% through orthogonal high-speed counter-current chromatography separation. UV spectroscopy, mass spectrometry and H-1 NMR spectroscopy were employed to identify their structures, which were assigned as L-tryptophan, quercetin-3-O-sophoroside, 5,7,3 ',4 '-tetrahydroxyflavonol-3-O-[beta-D-rhamnopyranosil-(1 -> 2)]-beta-D-glucopyranoside, and quercetin-3-O-glucoside. Each monomer exhibited significant antioxidant activity. The results demonstrated that proposed method could be an effective approach to isolate bioactive compounds from complex natural products. |
Keyword | Abelmoschus Esculentus (l). Moench Antioxidants Extrusion Elution Mode High-speed Counter-current Chromatography Wavelength Switching |
DOI | 10.1002/jssc.201600617 |
Indexed By | SCI |
Language | 英语 |
WOS ID | WOS:000389045100015 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.sinap.ac.cn/handle/331007/26648 |
Collection | 中科院上海应用物理研究所2011-2020年 |
Corresponding Author | Chen, XQ (reprint author), Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China. |
Recommended Citation GB/T 7714 | Wang, RH,Liu, Q,Wu, ZL,et al. Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(20):3983-3989. |
APA | Wang, RH,Liu, Q,Wu, ZL,Wang, ML,Chen, XQ,&Chen, XQ .(2016).Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode.JOURNAL OF SEPARATION SCIENCE,39(20),3983-3989. |
MLA | Wang, RH,et al."Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode".JOURNAL OF SEPARATION SCIENCE 39.20(2016):3983-3989. |
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