Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode

doi:10.1002/jssc.201600617

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	Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode
	Wang, RH; Liu, Q; Wu, ZL; Wang, ML; Chen, XQ; Chen, XQ (reprint author), Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
	2016
Source Publication	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9306
Volume	39 Issue:20 Pages:3983-3989
Subtype	期刊论文
Abstract	An off-line two-dimensional high-speed counter-current chromatography strategy combined with the wavelength switching technique and extrusion elution mode was successfully developed and applied to the isolation of polar antioxidants from Abelmoschus esculentus (L). Moench. Target-guided by the result of 2,2-diphenyl-1-picrylhydrazyl screening assay, four antioxidants were obtained with purities over 90% through orthogonal high-speed counter-current chromatography separation. UV spectroscopy, mass spectrometry and H-1 NMR spectroscopy were employed to identify their structures, which were assigned as L-tryptophan, quercetin-3-O-sophoroside, 5,7,3 ',4 '-tetrahydroxyflavonol-3-O-[beta-D-rhamnopyranosil-(1 -> 2)]-beta-D-glucopyranoside, and quercetin-3-O-glucoside. Each monomer exhibited significant antioxidant activity. The results demonstrated that proposed method could be an effective approach to isolate bioactive compounds from complex natural products.
Keyword	Abelmoschus Esculentus (l). Moench Antioxidants Extrusion Elution Mode High-speed Counter-current Chromatography Wavelength Switching
DOI	10.1002/jssc.201600617
Indexed By	SCI
Language	英语
WOS ID	WOS:000389045100015
Citation statistics
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/26648
Collection	中科院上海应用物理研究所2011-2018年
Corresponding Author	Chen, XQ (reprint author), Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
Recommended Citation GB/T 7714	Wang, RH,Liu, Q,Wu, ZL,et al. Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(20):3983-3989.
APA	Wang, RH,Liu, Q,Wu, ZL,Wang, ML,Chen, XQ,&Chen, XQ .(2016).Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode.JOURNAL OF SEPARATION SCIENCE,39(20),3983-3989.
MLA	Wang, RH,et al."Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode".JOURNAL OF SEPARATION SCIENCE 39.20(2016):3983-3989.

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