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题名:
Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode
作者: Wang, RH; Liu, Q; Wu, ZL; Wang, ML; Chen, XQ
刊名: JOURNAL OF SEPARATION SCIENCE
出版日期: 2016
卷号: 39, 期号:20, 页码:3983-3989
关键词: Abelmoschus esculentus (L). Moench ; Antioxidants ; Extrusion elution mode ; High-speed counter-current chromatography ; Wavelength switching
DOI: 10.1002/jssc.201600617
通讯作者: Chen, XQ (reprint author), Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
文章类型: 期刊论文
英文摘要: An off-line two-dimensional high-speed counter-current chromatography strategy combined with the wavelength switching technique and extrusion elution mode was successfully developed and applied to the isolation of polar antioxidants from Abelmoschus esculentus (L). Moench. Target-guided by the result of 2,2-diphenyl-1-picrylhydrazyl screening assay, four antioxidants were obtained with purities over 90% through orthogonal high-speed counter-current chromatography separation. UV spectroscopy, mass spectrometry and H-1 NMR spectroscopy were employed to identify their structures, which were assigned as L-tryptophan, quercetin-3-O-sophoroside, 5,7,3 ',4 '-tetrahydroxyflavonol-3-O-[beta-D-rhamnopyranosil-(1 -> 2)]-beta-D-glucopyranoside, and quercetin-3-O-glucoside. Each monomer exhibited significant antioxidant activity. The results demonstrated that proposed method could be an effective approach to isolate bioactive compounds from complex natural products.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000389045100015
ISSN号: 1615-9306
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/26648
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Wang, RH,Liu, Q,Wu, ZL,et al. Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode[J]. JOURNAL OF SEPARATION SCIENCE,2016-01-01,39(20):3983-3989.
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