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题名: The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution
作者: Li, N; Li, XH; Wang, YZ; Liu, GF; Zhou, P; Wu, HJ; Hong, CX; Bian, FG; Zhang, RG
刊名: JOURNAL OF APPLIED CRYSTALLOGRAPHY
出版日期: 2016
卷号: 49, 页码:1428-1432
关键词: biological small-angle X-ray scattering ; automation and high brilliance ; structural biology
DOI: 10.1107/S160057671601195X
通讯作者: Zhang, RG (reprint author), Shanghai Inst Biol Sci, Natl Ctr Prot Sci Shanghai, 320 Yueyang Rd, Shanghai 200031, Peoples R China. ; Bian, FG (reprint author), Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, 239 Zhangheng Rd, Shanghai 201204, Peoples R China.
文章类型: 期刊论文
英文摘要: The beamline BL19U2 is located in the Shanghai Synchrotron Radiation Facility (SSRF) and is its first beamline dedicated to biological material small-angle X-ray scattering (BioSAXS). The electrons come from an undulator which can provide high brilliance for the BL19U2 end stations. A double flat silicon crystal (111) monochromator is used in BL19U2, with a tunable monochromatic photon energy ranging from 7 to 15 keV. To meet the rapidly growing demands of crystallographers, biochemists and structural biologists, the BioSAXS beamline allows manual and automatic sample loading/unloading. A Pilatus 1M detector (Dectris) is employed for data collection, characterized by a high dynamic range and a short readout time. The highly automated data processing pipeline SASFLOW was integrated into BL19U2, with help from the BioSAXS group of the European Molecular Biology Laboratory (EMBL, Hamburg), which provides a user-friendly interface for data processing. The BL19U2 beamline was officially opened to users in March 2015. To date, feedback from users has been positive and the number of experimental proposals at BL19U2 is increasing. A description of the new BioSAXS beamline and the setup characteristics is given, together with examples of data obtained.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000388354800005
ISSN号: 1600-5767
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/26656
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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