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The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution
Li, N; Li, XH; Wang, YZ; Liu, GF; Zhou, P; Wu, HJ; Hong, CX; Bian, FG; Zhang, RG; Zhang, RG (reprint author), Shanghai Inst Biol Sci, Natl Ctr Prot Sci Shanghai, 320 Yueyang Rd, Shanghai 200031, Peoples R China.; Bian, FG (reprint author), Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, 239 Zhangheng Rd, Shanghai 201204, Peoples R China.
2016
Source PublicationJOURNAL OF APPLIED CRYSTALLOGRAPHY
ISSN1600-5767
Volume49Pages:1428-1432
Subtype期刊论文
AbstractThe beamline BL19U2 is located in the Shanghai Synchrotron Radiation Facility (SSRF) and is its first beamline dedicated to biological material small-angle X-ray scattering (BioSAXS). The electrons come from an undulator which can provide high brilliance for the BL19U2 end stations. A double flat silicon crystal (111) monochromator is used in BL19U2, with a tunable monochromatic photon energy ranging from 7 to 15 keV. To meet the rapidly growing demands of crystallographers, biochemists and structural biologists, the BioSAXS beamline allows manual and automatic sample loading/unloading. A Pilatus 1M detector (Dectris) is employed for data collection, characterized by a high dynamic range and a short readout time. The highly automated data processing pipeline SASFLOW was integrated into BL19U2, with help from the BioSAXS group of the European Molecular Biology Laboratory (EMBL, Hamburg), which provides a user-friendly interface for data processing. The BL19U2 beamline was officially opened to users in March 2015. To date, feedback from users has been positive and the number of experimental proposals at BL19U2 is increasing. A description of the new BioSAXS beamline and the setup characteristics is given, together with examples of data obtained.
KeywordBiological Small-angle X-ray Scattering Automation And High Brilliance Structural Biology
DOI10.1107/S160057671601195X
Indexed BySCI
Language英语
WOS IDWOS:000388354800005
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26656
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorZhang, RG (reprint author), Shanghai Inst Biol Sci, Natl Ctr Prot Sci Shanghai, 320 Yueyang Rd, Shanghai 200031, Peoples R China.; Bian, FG (reprint author), Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, 239 Zhangheng Rd, Shanghai 201204, Peoples R China.
Recommended Citation
GB/T 7714
Li, N,Li, XH,Wang, YZ,et al. The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY,2016,49:1428-1432.
APA Li, N.,Li, XH.,Wang, YZ.,Liu, GF.,Zhou, P.,...&Bian, FG .(2016).The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution.JOURNAL OF APPLIED CRYSTALLOGRAPHY,49,1428-1432.
MLA Li, N,et al."The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution".JOURNAL OF APPLIED CRYSTALLOGRAPHY 49(2016):1428-1432.
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