The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution
Li, N; Li, XH; Wang, YZ; Liu, GF; Zhou, P; Wu, HJ; Hong, CX; Bian, FG; Zhang, RG; Zhang, RG (reprint author), Shanghai Inst Biol Sci, Natl Ctr Prot Sci Shanghai, 320 Yueyang Rd, Shanghai 200031, Peoples R China.; Bian, FG (reprint author), Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, 239 Zhangheng Rd, Shanghai 201204, Peoples R China.
2016
发表期刊JOURNAL OF APPLIED CRYSTALLOGRAPHY
ISSN1600-5767
卷号49页码:1428-1432
文章类型期刊论文
摘要The beamline BL19U2 is located in the Shanghai Synchrotron Radiation Facility (SSRF) and is its first beamline dedicated to biological material small-angle X-ray scattering (BioSAXS). The electrons come from an undulator which can provide high brilliance for the BL19U2 end stations. A double flat silicon crystal (111) monochromator is used in BL19U2, with a tunable monochromatic photon energy ranging from 7 to 15 keV. To meet the rapidly growing demands of crystallographers, biochemists and structural biologists, the BioSAXS beamline allows manual and automatic sample loading/unloading. A Pilatus 1M detector (Dectris) is employed for data collection, characterized by a high dynamic range and a short readout time. The highly automated data processing pipeline SASFLOW was integrated into BL19U2, with help from the BioSAXS group of the European Molecular Biology Laboratory (EMBL, Hamburg), which provides a user-friendly interface for data processing. The BL19U2 beamline was officially opened to users in March 2015. To date, feedback from users has been positive and the number of experimental proposals at BL19U2 is increasing. A description of the new BioSAXS beamline and the setup characteristics is given, together with examples of data obtained.
关键词Biological Small-angle X-ray Scattering Automation And High Brilliance Structural Biology
DOI10.1107/S160057671601195X
收录类别SCI
语种英语
WOS记录号WOS:000388354800005
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/26656
专题中科院上海应用物理研究所2011-2018年
通讯作者Zhang, RG (reprint author), Shanghai Inst Biol Sci, Natl Ctr Prot Sci Shanghai, 320 Yueyang Rd, Shanghai 200031, Peoples R China.; Bian, FG (reprint author), Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, 239 Zhangheng Rd, Shanghai 201204, Peoples R China.
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Li, N,Li, XH,Wang, YZ,et al. The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY,2016,49:1428-1432.
APA Li, N.,Li, XH.,Wang, YZ.,Liu, GF.,Zhou, P.,...&Bian, FG .(2016).The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution.JOURNAL OF APPLIED CRYSTALLOGRAPHY,49,1428-1432.
MLA Li, N,et al."The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution".JOURNAL OF APPLIED CRYSTALLOGRAPHY 49(2016):1428-1432.
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