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题名: The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells
作者: Cui, FM; Liu, L; Zheng, LL; Bao, GL; Tu, Y; Sun, L; Zhu, W; Cao, JP; Zhou, PK; Chen, Q; He, YM
刊名: DOSE-RESPONSE
出版日期: 2016
卷号: 14, 期号:2, 页码:-
关键词: MiR-34a ; tritiated water ; DNA damage ; c-myc
DOI: 10.1177/1559325816638585
通讯作者: Chen, Q (reprint author), Soochow Univ, Sch Radiat Med & Protect, Dept Radiat Med, Coll Med, Suzhou 215123, Peoples R China. ; He, YM (reprint author), Soochow Univ, Dept Cardiol, Affiliated Hosp 1, Suzhou 215123, Peoples R China.
文章类型: 期刊论文
英文摘要: In this work, we investigated the toxic effects of tritiated water (HTO) on the cardiovascular system. We examined the role of microRNA-34a (miR-34a) in DNA damage and repair in human umbilical vein endothelial cells (HUVECs) exposed to HTO. Cell proliferation capacity was evaluated by cell counting, and miR-34a expression was detected using quantitative PCR (QT-PCR). The Comet assay and -H2AX immunostaining were used to measure DNA double-strand breaks (DSBs). Reverse transcription polymerase chain reaction was used to measure the expression level of c-myc messenger RNA (mRNA). The cells exposed to HTO showed significantly lower proliferation than the control cells over 3 days. The DNA damage in the HTO group was more severe than that in the control group, at each time point examined. The expression of miR-34a mimics caused increased DNA DSBs whereas that of the miR-34a inhibitor caused decreased DNA DSBs. The proliferation viability was the opposite for the miR-34a mimics and inhibitor groups. The expression levels of c-myc mRNA in cells transfected with miR-34a mimics were lower than that in cells transfected with the miR-34a-5p inhibitor, at 0.5 hours and 2 hours after transfection. In summary, miR-34a mediates HTO toxicity in HUVECs by downregulating the expression of c-myc.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000380136800001
ISSN号: 1559-3258
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/26657
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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