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The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells
Cui, FM; Liu, L; Zheng, LL; Bao, GL; Tu, Y; Sun, L; Zhu, W; Cao, JP; Zhou, PK; Chen, Q; He, YM; Chen, Q (reprint author), Soochow Univ, Sch Radiat Med & Protect, Dept Radiat Med, Coll Med, Suzhou 215123, Peoples R China.; He, YM (reprint author), Soochow Univ, Dept Cardiol, Affiliated Hosp 1, Suzhou 215123, Peoples R China.
2016
Source PublicationDOSE-RESPONSE
ISSN1559-3258
Volume14Issue:2Pages:-
Subtype期刊论文
AbstractIn this work, we investigated the toxic effects of tritiated water (HTO) on the cardiovascular system. We examined the role of microRNA-34a (miR-34a) in DNA damage and repair in human umbilical vein endothelial cells (HUVECs) exposed to HTO. Cell proliferation capacity was evaluated by cell counting, and miR-34a expression was detected using quantitative PCR (QT-PCR). The Comet assay and -H2AX immunostaining were used to measure DNA double-strand breaks (DSBs). Reverse transcription polymerase chain reaction was used to measure the expression level of c-myc messenger RNA (mRNA). The cells exposed to HTO showed significantly lower proliferation than the control cells over 3 days. The DNA damage in the HTO group was more severe than that in the control group, at each time point examined. The expression of miR-34a mimics caused increased DNA DSBs whereas that of the miR-34a inhibitor caused decreased DNA DSBs. The proliferation viability was the opposite for the miR-34a mimics and inhibitor groups. The expression levels of c-myc mRNA in cells transfected with miR-34a mimics were lower than that in cells transfected with the miR-34a-5p inhibitor, at 0.5 hours and 2 hours after transfection. In summary, miR-34a mediates HTO toxicity in HUVECs by downregulating the expression of c-myc.
KeywordMir-34a Tritiated Water Dna Damage C-myc
DOI10.1177/1559325816638585
Indexed BySCI
Language英语
WOS IDWOS:000380136800001
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/26657
Collection中科院上海应用物理研究所2011-2018年
Corresponding AuthorChen, Q (reprint author), Soochow Univ, Sch Radiat Med & Protect, Dept Radiat Med, Coll Med, Suzhou 215123, Peoples R China.; He, YM (reprint author), Soochow Univ, Dept Cardiol, Affiliated Hosp 1, Suzhou 215123, Peoples R China.
Recommended Citation
GB/T 7714
Cui, FM,Liu, L,Zheng, LL,et al. The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells[J]. DOSE-RESPONSE,2016,14(2):-.
APA Cui, FM.,Liu, L.,Zheng, LL.,Bao, GL.,Tu, Y.,...&He, YM .(2016).The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells.DOSE-RESPONSE,14(2),-.
MLA Cui, FM,et al."The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells".DOSE-RESPONSE 14.2(2016):-.
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