CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
An Exonuclease III-Powered, On-Particle Stochastic DNA Walker
Qu, XM; Zhu, D; Yao, GB; Su, S; Chao, J; Liu, HJ; Zuo, XL; Wang, LH; Shi, JY; Wang, LH; Huang, W; Pei, H; Fan, CH
AbstractDNA-based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonucleaseIII (ExoIII)-powered stochastic DNA walker that can autonomously move on a spherical nucleic acid (SNA)-based 3D track. The motion is propelled by unidirectional ExoIII digestion of hybridized DNA tracks in a burnt-bridge mechanism. The operation of this ExoIII-propelled DNA walker was monitored in real time and at the single-particle resolution using total internal reflection fluorescence microscopy (TIRF). We further interrogated the morphological effect of the 3D track on the nuclease activity, which suggested that the performance of the DNA walker was critically dependent upon the DNA density and the track conformation. Finally, we demonstrated potential bioanalytical applications of this SNA-based stochastic DNA walker by exploiting movement-triggered cascade signal amplification.
KeywordBrownian Motions Dna Nanotechnology Enzymes Molecular Machines Nanoparticles
Indexed BySCI
WOS IDWOS:000395554900026
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Cited Times:226[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
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GB/T 7714
Qu, XM,Zhu, D,Yao, GB,et al. An Exonuclease III-Powered, On-Particle Stochastic DNA Walker[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(7):1855-1858.
APA Qu, XM.,Zhu, D.,Yao, GB.,Su, S.,Chao, J.,...&Fan, CH.(2017).An Exonuclease III-Powered, On-Particle Stochastic DNA Walker.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(7),1855-1858.
MLA Qu, XM,et al."An Exonuclease III-Powered, On-Particle Stochastic DNA Walker".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.7(2017):1855-1858.
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