CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
An Exonuclease III-Powered, On-Particle Stochastic DNA Walker
Qu, XM; Zhu, D; Yao, GB; Su, S; Chao, J; Liu, HJ; Zuo, XL; Wang, LH; Shi, JY; Wang, LH; Huang, W; Pei, H; Fan, CH
2017
Source PublicationANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN1433-7851
Volume56Issue:7Pages:1855-1858
Subtype期刊论文
AbstractDNA-based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonucleaseIII (ExoIII)-powered stochastic DNA walker that can autonomously move on a spherical nucleic acid (SNA)-based 3D track. The motion is propelled by unidirectional ExoIII digestion of hybridized DNA tracks in a burnt-bridge mechanism. The operation of this ExoIII-propelled DNA walker was monitored in real time and at the single-particle resolution using total internal reflection fluorescence microscopy (TIRF). We further interrogated the morphological effect of the 3D track on the nuclease activity, which suggested that the performance of the DNA walker was critically dependent upon the DNA density and the track conformation. Finally, we demonstrated potential bioanalytical applications of this SNA-based stochastic DNA walker by exploiting movement-triggered cascade signal amplification.
KeywordBrownian Motions Dna Nanotechnology Enzymes Molecular Machines Nanoparticles
DOI10.1002/anie.201611777
Indexed BySCI
Language英语
WOS IDWOS:000395554900026
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/27252
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Qu, XM,Zhu, D,Yao, GB,et al. An Exonuclease III-Powered, On-Particle Stochastic DNA Walker[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(7):1855-1858.
APA Qu, XM.,Zhu, D.,Yao, GB.,Su, S.,Chao, J.,...&Fan, CH.(2017).An Exonuclease III-Powered, On-Particle Stochastic DNA Walker.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(7),1855-1858.
MLA Qu, XM,et al."An Exonuclease III-Powered, On-Particle Stochastic DNA Walker".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.7(2017):1855-1858.
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