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题名:
An Exonuclease III-Powered, On-Particle Stochastic DNA Walker
作者: Qu, XM; Zhu, D; Yao, GB; Su, S; Chao, J; Liu, HJ; Zuo, XL; Wang, LH; Shi, JY; Wang, LH; Huang, W; Pei, H; Fan, CH
刊名: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
出版日期: 2017
卷号: 56, 期号:7, 页码:1855-1858
关键词: Brownian motions ; DNA nanotechnology ; enzymes ; molecular machines ; nanoparticles
DOI: 10.1002/anie.201611777
文章类型: 期刊论文
英文摘要: DNA-based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonucleaseIII (ExoIII)-powered stochastic DNA walker that can autonomously move on a spherical nucleic acid (SNA)-based 3D track. The motion is propelled by unidirectional ExoIII digestion of hybridized DNA tracks in a burnt-bridge mechanism. The operation of this ExoIII-propelled DNA walker was monitored in real time and at the single-particle resolution using total internal reflection fluorescence microscopy (TIRF). We further interrogated the morphological effect of the 3D track on the nuclease activity, which suggested that the performance of the DNA walker was critically dependent upon the DNA density and the track conformation. Finally, we demonstrated potential bioanalytical applications of this SNA-based stochastic DNA walker by exploiting movement-triggered cascade signal amplification.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000395554900026
ISSN号: 1433-7851
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/27252
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Qu, XM,Zhu, D,Yao, GB,et al. An Exonuclease III-Powered, On-Particle Stochastic DNA Walker[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017-01-01,56(7):1855-1858.
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文件名: An Exonuclease III-Powered, On-Particle Stochastic DNA Walker.pdf
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