An Exonuclease III-Powered, On-Particle Stochastic DNA Walker
Qu, XM; Zhu, D; Yao, GB; Su, S; Chao, J; Liu, HJ; Zuo, XL; Wang, LH; Shi, JY; Wang, LH; Huang, W; Pei, H; Fan, CH
2017
发表期刊ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN1433-7851
卷号56期号:7页码:1855-1858
文章类型期刊论文
摘要DNA-based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonucleaseIII (ExoIII)-powered stochastic DNA walker that can autonomously move on a spherical nucleic acid (SNA)-based 3D track. The motion is propelled by unidirectional ExoIII digestion of hybridized DNA tracks in a burnt-bridge mechanism. The operation of this ExoIII-propelled DNA walker was monitored in real time and at the single-particle resolution using total internal reflection fluorescence microscopy (TIRF). We further interrogated the morphological effect of the 3D track on the nuclease activity, which suggested that the performance of the DNA walker was critically dependent upon the DNA density and the track conformation. Finally, we demonstrated potential bioanalytical applications of this SNA-based stochastic DNA walker by exploiting movement-triggered cascade signal amplification.
关键词Brownian Motions Dna Nanotechnology Enzymes Molecular Machines Nanoparticles
DOI10.1002/anie.201611777
收录类别SCI
语种英语
WOS记录号WOS:000395554900026
引用统计
被引频次:66[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/27252
专题中科院上海应用物理研究所2011-2018年
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Qu, XM,Zhu, D,Yao, GB,et al. An Exonuclease III-Powered, On-Particle Stochastic DNA Walker[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(7):1855-1858.
APA Qu, XM.,Zhu, D.,Yao, GB.,Su, S.,Chao, J.,...&Fan, CH.(2017).An Exonuclease III-Powered, On-Particle Stochastic DNA Walker.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(7),1855-1858.
MLA Qu, XM,et al."An Exonuclease III-Powered, On-Particle Stochastic DNA Walker".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.7(2017):1855-1858.
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