DNA origami-based shape IDs for single-molecule nanomechanical genotyping
Zhang, HL; Chao, J; Pan, D; Liu, HJ; Qiang, Y; Liu, K; Cui, CJ; Chen, JH; Huang, Q; Hu, J; Wang, LH; Huang, W; Shi, YY; Fan, CH
2017
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
卷号8页码:-
文章类型期刊论文
摘要Variations on DNA sequences profoundly affect how we develop diseases and respond to pathogens and drugs. Atomic force microscopy (AFM) provides a nanomechanical imaging approach for genetic analysis with nanometre resolution. However, unlike fluorescence imaging that has wavelength-specific fluorophores, the lack of shape-specific labels largely hampers widespread applications of AFM imaging. Here we report the development of a set of differentially shaped, highly hybridizable self-assembled DNA origami nanostructures serving as shape IDs for magnified nanomechanical imaging of single-nucleotide polymorphisms. Using these origami shape IDs, we directly genotype single molecules of human genomic DNA with an ultrahigh resolution of similar to 10nm and the multiplexing ability. Further, we determine three types of disease-associated, long-range haplotypes in samples from the Han Chinese population. Single-molecule analysis allows robust haplotyping even for samples with low labelling efficiency. We expect this generic shape ID-based nanomechanical approach to hold great potential in genetic analysis at the single-molecule level.
DOI10.1038/ncomms14738
收录类别SCI
语种英语
WOS记录号WOS:000398410000001
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/27300
专题中科院上海应用物理研究所2011-2018年
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Zhang, HL,Chao, J,Pan, D,et al. DNA origami-based shape IDs for single-molecule nanomechanical genotyping[J]. NATURE COMMUNICATIONS,2017,8:-.
APA Zhang, HL.,Chao, J.,Pan, D.,Liu, HJ.,Qiang, Y.,...&Fan, CH.(2017).DNA origami-based shape IDs for single-molecule nanomechanical genotyping.NATURE COMMUNICATIONS,8,-.
MLA Zhang, HL,et al."DNA origami-based shape IDs for single-molecule nanomechanical genotyping".NATURE COMMUNICATIONS 8(2017):-.
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