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CAS OpenIR  > 中科院上海应用物理研究所2011-2017年  > 期刊论文
题名:
Expression and radiolabeling of Cas9 protein
作者: Yan, QL; Kong, HT; Xia, K; Zhang, Y; Aladlbahi, A; Shi, JY; Wang, LH; Fan, CH; Zhao, Y; Zhu, Y
刊名: NUCLEAR SCIENCE AND TECHNIQUES
出版日期: 2017
卷号: 28, 期号:1, 页码:-
关键词: Cas9 ; Radiolabeling ; I-125 labeling yield ; Stability
DOI: 10.1007/s41365-016-0165-8
文章类型: 期刊论文
英文摘要: As a robust platform for genome editing, CRISPR/Cas9 is currently being explored for engineering biology or therapeutics, yet means for quantitative detection of Cas9 proteins remain to be fully realized. Here, we expressed Cas9 proteins and developed a novel detection method that traced Cas9 based on radiolabeled iodine. Through optimizing the reaction conditions of reaction time, temperature and cycles, we obtained I-125-Cas9 of high labeling yield. The prepared I-125-Cas9 was stable in various media and preserved excellent genome editing efficiency. Thus, our strategy provides a convenient and efficient tool for further tracing biological behaviors of Cas9 proteins in living systems.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000393150100007
ISSN号: 1001-8042
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/27319
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Yan, QL,Kong, HT,Xia, K,et al. Expression and radiolabeling of Cas9 protein[J]. NUCLEAR SCIENCE AND TECHNIQUES,2017-01-01,28(1):-.
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