CAS OpenIR  > 中科院上海应用物理研究所2011-2020年
Expression and radiolabeling of Cas9 protein
Yan, QL; Kong, HT; Xia, K; Zhang, Y; Aladlbahi, A; Shi, JY; Wang, LH; Fan, CH; Zhao, Y; Zhu, Y
AbstractAs a robust platform for genome editing, CRISPR/Cas9 is currently being explored for engineering biology or therapeutics, yet means for quantitative detection of Cas9 proteins remain to be fully realized. Here, we expressed Cas9 proteins and developed a novel detection method that traced Cas9 based on radiolabeled iodine. Through optimizing the reaction conditions of reaction time, temperature and cycles, we obtained I-125-Cas9 of high labeling yield. The prepared I-125-Cas9 was stable in various media and preserved excellent genome editing efficiency. Thus, our strategy provides a convenient and efficient tool for further tracing biological behaviors of Cas9 proteins in living systems.
KeywordCas9 Radiolabeling I-125 Labeling Yield Stability
Indexed BySCI
WOS IDWOS:000393150100007
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GB/T 7714
Yan, QL,Kong, HT,Xia, K,et al. Expression and radiolabeling of Cas9 protein[J]. NUCLEAR SCIENCE AND TECHNIQUES,2017,28(1):-.
APA Yan, QL.,Kong, HT.,Xia, K.,Zhang, Y.,Aladlbahi, A.,...&Zhu, Y.(2017).Expression and radiolabeling of Cas9 protein.NUCLEAR SCIENCE AND TECHNIQUES,28(1),-.
MLA Yan, QL,et al."Expression and radiolabeling of Cas9 protein".NUCLEAR SCIENCE AND TECHNIQUES 28.1(2017):-.
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