Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification

doi:10.1021/acsami.7b01134

CAS OpenIR > 中科院上海应用物理研究所2011-2018年

	Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification
	Li, F; Liu, XG; Zhao, B; Yan, J; Li, Q; Aldalbahi, A; Shi, JY; Song, SP; Fan, CH; Wang, LH
	2017
发表期刊	ACS APPLIED MATERIALS & INTERFACES
ISSN	1944-8244
卷号	9 期号:18 页码:15245-15253
文章类型	期刊论文
摘要	Isothermal amplification is an efficient way to amplify DNA with high accuracy; however, the real-time monitoring for quantification analysis mostly relied on expensive and precisely dsDNA designed probes. In the present study, a graphene oxide (GO)-based nanoprobe was used to real-time monitor the isothermal amplification process. The interaction between GO and different DNA structures was systematically investigated, including single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), DNA 3-helix, and long rolling circle amplification (RCA) and hybridization chain reaction (HCR) products, which existed in one-, two-, and three-dimensional structures. It was found that the high rigid structures exhibited much lower affinity with GO than soft ssDNA, and generally the rigidity was dependent on the length of targets and the hybridization position with probe DNA. On the basis of these results, we successfully monitored HCR amplification process, RCA process, and the enzyme restriction of RCA products with GO nanoprobe; other applications including the detection of the assembly/disassembly of DNA 3-helix structures were also performed. Compared to the widely used end-point detection methods, the GO-based sensing platform is simple, sensitive, cost-effective, and especially in a real-time monitoring mode. We believe such studies can provide comprehensive understandings and evocation on design of GO-based biosensors for broad application in various fields.
关键词	Interaction Of Graphene Oxide And Dna Length And Position Dependent Various Dna Structures Real-time Detection Isothermal Amplification
DOI	10.1021/acsami.7b01134
收录类别	SCI
语种	英语
WOS记录号	WOS:000401307100005
引用统计
文献类型	期刊论文
条目标识符	http://ir.sinap.ac.cn/handle/331007/27336
专题	中科院上海应用物理研究所2011-2018年
推荐引用方式 GB/T 7714	Li, F,Liu, XG,Zhao, B,et al. Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(18):15245-15253.
APA	Li, F.,Liu, XG.,Zhao, B.,Yan, J.,Li, Q.,...&Wang, LH.(2017).Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification.ACS APPLIED MATERIALS & INTERFACES,9(18),15245-15253.
MLA	Li, F,et al."Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification".ACS APPLIED MATERIALS & INTERFACES 9.18(2017):15245-15253.

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