Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification
Li, F; Liu, XG; Zhao, B; Yan, J; Li, Q; Aldalbahi, A; Shi, JY; Song, SP; Fan, CH; Wang, LH
2017
发表期刊ACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
卷号9期号:18页码:15245-15253
文章类型期刊论文
摘要Isothermal amplification is an efficient way to amplify DNA with high accuracy; however, the real-time monitoring for quantification analysis mostly relied on expensive and precisely dsDNA designed probes. In the present study, a graphene oxide (GO)-based nanoprobe was used to real-time monitor the isothermal amplification process. The interaction between GO and different DNA structures was systematically investigated, including single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), DNA 3-helix, and long rolling circle amplification (RCA) and hybridization chain reaction (HCR) products, which existed in one-, two-, and three-dimensional structures. It was found that the high rigid structures exhibited much lower affinity with GO than soft ssDNA, and generally the rigidity was dependent on the length of targets and the hybridization position with probe DNA. On the basis of these results, we successfully monitored HCR amplification process, RCA process, and the enzyme restriction of RCA products with GO nanoprobe; other applications including the detection of the assembly/disassembly of DNA 3-helix structures were also performed. Compared to the widely used end-point detection methods, the GO-based sensing platform is simple, sensitive, cost-effective, and especially in a real-time monitoring mode. We believe such studies can provide comprehensive understandings and evocation on design of GO-based biosensors for broad application in various fields.
关键词Interaction Of Graphene Oxide And Dna Length And Position Dependent Various Dna Structures Real-time Detection Isothermal Amplification
DOI10.1021/acsami.7b01134
收录类别SCI
语种英语
WOS记录号WOS:000401307100005
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/27336
专题中科院上海应用物理研究所2011-2018年
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GB/T 7714
Li, F,Liu, XG,Zhao, B,et al. Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(18):15245-15253.
APA Li, F.,Liu, XG.,Zhao, B.,Yan, J.,Li, Q.,...&Wang, LH.(2017).Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification.ACS APPLIED MATERIALS & INTERFACES,9(18),15245-15253.
MLA Li, F,et al."Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification".ACS APPLIED MATERIALS & INTERFACES 9.18(2017):15245-15253.
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