Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Identification of a nanobody specific to human pulmonary surfactant protein A | |
| He, X; Wang, SM; Yin, ZF; Zhao, MM; Li, N; Yu, F; Wang, LS; Hu, Y; Du, YK; Du, SS; Li, Y; Wei, YR; Chen, SS; He, JH; Weng, D; Li, HP | |
| 2017 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 7Pages:- |
| Subtype | 期刊论文 |
| Abstract | Nanobody (Nb) is a promising vector for targeted drug delivery. This study aims to identify an Nb that can specifically target the lung by binding human pulmonary surfactant protein A (SP-A). Human lung frozen tissue sections were used for 3 rounds of biospanning of our previously constructed Nb library for rat SP-A to establish a sub-library of Nb, which specifically bound human lung tissues. Phage-ELISA was performed to screen the sub-library to identify Nb4, which specifically bound human SP-A. The binding affinity Kd of Nb4 to recombinant human SP-A was 7.48 x 10(-7) M. Nb4 (19kDa) was stable at 30 degrees C-37 degrees C and pH 7.0-7.6 and specifically bound the SP-A in human lung tissue homogenates, human lung A549 cells, and human lung tissues, whereas didn't react with human liver L-02 cells, kidney 293T cells, and human tissues from organs other than the lung. Nb4 accumulated in the lung of nude mice 5 minutes after a tail vein injection of Nb4 and was excreted 3 hours. Short-term exposure ( one month) to Nb4 didn't cause apparent liver and kidney toxicity in rats, whereas 3-month exposure resulted in mild liver and kidney injuries. Nb4 may be a promising vector to specifically deliver drugs to the lung. |
| DOI | 10.1038/s41598-017-01456-2 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000400491100013 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/27347 |
| Collection | 中科院上海应用物理研究所2011-2019年 |
| Recommended Citation GB/T 7714 | He, X,Wang, SM,Yin, ZF,et al. Identification of a nanobody specific to human pulmonary surfactant protein A[J]. SCIENTIFIC REPORTS,2017,7:-. |
| APA | He, X.,Wang, SM.,Yin, ZF.,Zhao, MM.,Li, N.,...&Li, HP.(2017).Identification of a nanobody specific to human pulmonary surfactant protein A.SCIENTIFIC REPORTS,7,-. |
| MLA | He, X,et al."Identification of a nanobody specific to human pulmonary surfactant protein A".SCIENTIFIC REPORTS 7(2017):-. |
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| Identification of a （5831KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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