Neovascularization of hepatocellular carcinoma in a nude mouse orthotopic liver cancer model: a morphological study using X-ray in-line phase-contrast imaging
Li, BL; Zhang, YQ; Wu, WZ; Du, GH; Cai, L; Shi, HC; Chen, SL
2017
发表期刊BMC CANCER
ISSN1471-2407
卷号17页码:-
文章类型期刊论文
摘要Background: This study aimed to determine whether synchrotron radiation (SR)-based X-ray in-line phase-contrast imaging (IL-PCI) can be used to investigate the morphological characteristics of tumor neovascularization in a liver xenograft animal model. Methods: A human hepatocellular carcinoma HCCLM3 xenograft model was established in nude mice. Xenografts were sampled each week for 4 weeks and fixed to analyze tissue characteristics and neovascularization using SR-based X-ray in-line phase contrast computed tomography (IL-XPCT) without any contrast agent. Results: The effect of the energy level and object-to-detector distance on phase-contrast difference was in good agreement with the theory of IL-PCI. Boundaries between the tumor and adjacent normal tissues at week 1 were clearly observed in two-dimensional phase contrast projection imaging. A quantitative contrast difference was observed from weeks 1 to 4. Moreover, 3D image reconstruction of hepatocellular carcinoma (HCC) samples showed blood vessels inside the tumor were abnormal. The smallest blood vessels measured approximately 20 mu m in diameter. The tumor vascular density initially increased and then decreased gradually over time. The maximum tumor vascular density was 4.29% at week 2. Conclusion: IL-XPCT successfully acquired images of neovascularization in HCC xenografts in nude mice.
关键词Synchrotron Radiation In-line Phase-contrast Imaging Computed Tomography Hepatocellular Carcinoma Tumor Neovascularization
DOI10.1186/s12885-017-3073-3
收录类别SCI
语种英语
WOS记录号WOS:000393840200001
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/27393
专题中科院上海应用物理研究所2011-2018年
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Li, BL,Zhang, YQ,Wu, WZ,et al. Neovascularization of hepatocellular carcinoma in a nude mouse orthotopic liver cancer model: a morphological study using X-ray in-line phase-contrast imaging[J]. BMC CANCER,2017,17:-.
APA Li, BL.,Zhang, YQ.,Wu, WZ.,Du, GH.,Cai, L.,...&Chen, SL.(2017).Neovascularization of hepatocellular carcinoma in a nude mouse orthotopic liver cancer model: a morphological study using X-ray in-line phase-contrast imaging.BMC CANCER,17,-.
MLA Li, BL,et al."Neovascularization of hepatocellular carcinoma in a nude mouse orthotopic liver cancer model: a morphological study using X-ray in-line phase-contrast imaging".BMC CANCER 17(2017):-.
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