CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Silicone oil promotes amyloid-like aggregation of alpha B-crystallin
Shen, ZW; Du, QQG; Lei, HZ; Wei, YH; Hu, J; Zhang, Y
2017
Source PublicationRSC ADVANCES
ISSN2046-2069
Volume7Issue:10Pages:6000-6005
Subtype期刊论文
AbstractSilicone oil is a chemically inert and biocompatible material. However, the use of silicone oil as an adjunct for internal tamponade in the treatment of retinal detachment is accompanied by the sequelae of cataract, the molecular mechanism of which has been a mystery for scientists. In this study, we focused on the influence of silicone oil on the aggregating behaviors of one of the important proteins in the eyes, alpha B-crystallin (CRYAB). We found that silicone oil could promote the amyloid-like aggregation of CRYAB, verified using atomic force microscopy (AFM), transmission electron microscopy (TEM), laser granularity and number analysis (Nanosight), and turbidity measurements. Furthermore, fluorescent experiments using Thioflavin T (ThT), Congo red, and 1-anilinonaphthalene-8-sulfonic acid (ANS) suggested formation of a beta-sheet structure in CRYAB in the presence of silicone oil, which was also confirmed by far-UV circular dichroism (CD) spectroscopy. These findings provide a direct evidence of the changes in the secondary structures of CRYAB protein and amyloid aggregation behavior upon adding silicone oil in the solution, and could be helpful for understanding the molecular mechanisms of the cataract formed in silicone oil-filled eyes.
DOI10.1039/c6ra27128f
Indexed BySCI
Language英语
WOS IDWOS:000393754100054
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/27441
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Shen, ZW,Du, QQG,Lei, HZ,et al. Silicone oil promotes amyloid-like aggregation of alpha B-crystallin[J]. RSC ADVANCES,2017,7(10):6000-6005.
APA Shen, ZW,Du, QQG,Lei, HZ,Wei, YH,Hu, J,&Zhang, Y.(2017).Silicone oil promotes amyloid-like aggregation of alpha B-crystallin.RSC ADVANCES,7(10),6000-6005.
MLA Shen, ZW,et al."Silicone oil promotes amyloid-like aggregation of alpha B-crystallin".RSC ADVANCES 7.10(2017):6000-6005.
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