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题名:
Silicone oil promotes amyloid-like aggregation of alpha B-crystallin
作者: Shen, ZW; Du, QQG; Lei, HZ; Wei, YH; Hu, J; Zhang, Y
刊名: RSC ADVANCES
出版日期: 2017
卷号: 7, 期号:10, 页码:6000-6005
DOI: 10.1039/c6ra27128f
文章类型: 期刊论文
英文摘要: Silicone oil is a chemically inert and biocompatible material. However, the use of silicone oil as an adjunct for internal tamponade in the treatment of retinal detachment is accompanied by the sequelae of cataract, the molecular mechanism of which has been a mystery for scientists. In this study, we focused on the influence of silicone oil on the aggregating behaviors of one of the important proteins in the eyes, alpha B-crystallin (CRYAB). We found that silicone oil could promote the amyloid-like aggregation of CRYAB, verified using atomic force microscopy (AFM), transmission electron microscopy (TEM), laser granularity and number analysis (Nanosight), and turbidity measurements. Furthermore, fluorescent experiments using Thioflavin T (ThT), Congo red, and 1-anilinonaphthalene-8-sulfonic acid (ANS) suggested formation of a beta-sheet structure in CRYAB in the presence of silicone oil, which was also confirmed by far-UV circular dichroism (CD) spectroscopy. These findings provide a direct evidence of the changes in the secondary structures of CRYAB protein and amyloid aggregation behavior upon adding silicone oil in the solution, and could be helpful for understanding the molecular mechanisms of the cataract formed in silicone oil-filled eyes.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000393754100054
ISSN号: 2046-2069
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/27441
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Shen, ZW,Du, QQG,Lei, HZ,et al. Silicone oil promotes amyloid-like aggregation of alpha B-crystallin[J]. RSC ADVANCES,2017-01-01,7(10):6000-6005.
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