Structural consistency analysis of recombinant and wild-type human serum albumin
Cao, HL; Sun, LH; Liu, L; Li, J; Tang, L; Guo, YZ; Mei, QB; He, JH; Yin, DC
2017
发表期刊JOURNAL OF MOLECULAR STRUCTURE
ISSN0022-2860
卷号1127页码:1-5
文章类型期刊论文
摘要Recombinant human serum albumin (rHSA) is potential alternatives for human serum albumin (HSA) which may ease severe shortage of HSA worldwide. In theory, rHSA and HSA are the same. Structure decides function. Therefore, the 3D structural consistency analysis of rHSA and HSA is outmost importance, which is the base of their function consistency. In this paper, the crystal structures of rHSA at resolution limit of 2.22 angstrom and HSA at 230 angstrom were determined by X-ray diffraction (XRD), which were deposited in the Protein Data Bank (PDB) with accession codes 4G03 (rHSA) and 4G04 (HSA). The differences between rHSA and HSA were systematically analyzed from the crystallization behavior, diffraction data and three-dimensional (3D) structure. The superimposed contrasted analysis indicated that rHSA and HSA achieved a structural similarity of 99% with an r.m.s. deviation of 0.397 angstrom for the corresponding overall C-alpha atoms. In addition, the number of alpha-helices in the rHSA or HSA molecule was verified to be 30. As a result, rHSA can potentially replace HSA. The study provides a theoretical and experimental basis for the clinical and additional applications of rHSA. Meanwhile, it is also a good example for applications of genetic engineering. (C) 2016 Elsevier B.V. All rights reserved.
关键词Crystal Structure Consistency Analysis Wild-type Human Serum Albumin (Hsa) Recombinant Human Serum Albumin (Rhsa)
DOI10.1016/j.molstruc.2016.07.057
收录类别SCI
语种英语
WOS记录号WOS:000385901800001
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/27451
专题中科院上海应用物理研究所2011-2018年
推荐引用方式
GB/T 7714
Cao, HL,Sun, LH,Liu, L,et al. Structural consistency analysis of recombinant and wild-type human serum albumin[J]. JOURNAL OF MOLECULAR STRUCTURE,2017,1127:1-5.
APA Cao, HL.,Sun, LH.,Liu, L.,Li, J.,Tang, L.,...&Yin, DC.(2017).Structural consistency analysis of recombinant and wild-type human serum albumin.JOURNAL OF MOLECULAR STRUCTURE,1127,1-5.
MLA Cao, HL,et al."Structural consistency analysis of recombinant and wild-type human serum albumin".JOURNAL OF MOLECULAR STRUCTURE 1127(2017):1-5.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Structural consisten(1310KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cao, HL]的文章
[Sun, LH]的文章
[Liu, L]的文章
百度学术
百度学术中相似的文章
[Cao, HL]的文章
[Sun, LH]的文章
[Liu, L]的文章
必应学术
必应学术中相似的文章
[Cao, HL]的文章
[Sun, LH]的文章
[Liu, L]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。