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Structural consistency analysis of recombinant and wild-type human serum albumin
Cao, HL; Sun, LH; Liu, L; Li, J; Tang, L; Guo, YZ; Mei, QB; He, JH; Yin, DC
2017
Source PublicationJOURNAL OF MOLECULAR STRUCTURE
ISSN0022-2860
Volume1127Pages:1-5
Subtype期刊论文
AbstractRecombinant human serum albumin (rHSA) is potential alternatives for human serum albumin (HSA) which may ease severe shortage of HSA worldwide. In theory, rHSA and HSA are the same. Structure decides function. Therefore, the 3D structural consistency analysis of rHSA and HSA is outmost importance, which is the base of their function consistency. In this paper, the crystal structures of rHSA at resolution limit of 2.22 angstrom and HSA at 230 angstrom were determined by X-ray diffraction (XRD), which were deposited in the Protein Data Bank (PDB) with accession codes 4G03 (rHSA) and 4G04 (HSA). The differences between rHSA and HSA were systematically analyzed from the crystallization behavior, diffraction data and three-dimensional (3D) structure. The superimposed contrasted analysis indicated that rHSA and HSA achieved a structural similarity of 99% with an r.m.s. deviation of 0.397 angstrom for the corresponding overall C-alpha atoms. In addition, the number of alpha-helices in the rHSA or HSA molecule was verified to be 30. As a result, rHSA can potentially replace HSA. The study provides a theoretical and experimental basis for the clinical and additional applications of rHSA. Meanwhile, it is also a good example for applications of genetic engineering. (C) 2016 Elsevier B.V. All rights reserved.
KeywordCrystal Structure Consistency Analysis Wild-type Human Serum Albumin (Hsa) Recombinant Human Serum Albumin (Rhsa)
DOI10.1016/j.molstruc.2016.07.057
Indexed BySCI
Language英语
WOS IDWOS:000385901800001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/27451
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Cao, HL,Sun, LH,Liu, L,et al. Structural consistency analysis of recombinant and wild-type human serum albumin[J]. JOURNAL OF MOLECULAR STRUCTURE,2017,1127:1-5.
APA Cao, HL.,Sun, LH.,Liu, L.,Li, J.,Tang, L.,...&Yin, DC.(2017).Structural consistency analysis of recombinant and wild-type human serum albumin.JOURNAL OF MOLECULAR STRUCTURE,1127,1-5.
MLA Cao, HL,et al."Structural consistency analysis of recombinant and wild-type human serum albumin".JOURNAL OF MOLECULAR STRUCTURE 1127(2017):1-5.
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