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Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles
Du, QQG; Li, WR; Yuan, M; Gong, P; Zhang, Y; Zhang, F
2017
Source PublicationLUMINESCENCE
ISSN1522-7235
Volume32Issue:4Pages:618-624
Subtype期刊论文
AbstractPhysisorptions occurs everywhere and constantly in living organisms and between nanomaterials and biomolecules. In this study, one of the most important proteins, G-actin, was selected to investigate its bio-nano physisorption with a model nanoparticle coated with a amphiphilic polymer. Using a photoluminescence quenching method, both the binding constant and the Hill constant were determined as 1.79x10(7)M(-1) and 0.84, respectively. Thermodynamic calculations proved that such a physisorption was a spontaneous procedure. The physisorption-mediated protein-nanoparticle conjugates were robust enough to resist gel electrophoresis, and protein conformation was kept intact, as revealed using circular dichroism. This conjugate might be a promising candidate for nanofabrication or could play a significant role in actin-related bioactivities.
KeywordAmphiphilic Polymer Binding Constant G-actin Gold Nanoparticle Photoluminescence Quenching
DOI10.1002/bio.3229
Indexed BySCI
Language英语
WOS IDWOS:000401144200020
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/27455
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Du, QQG,Li, WR,Yuan, M,et al. Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles[J]. LUMINESCENCE,2017,32(4):618-624.
APA Du, QQG,Li, WR,Yuan, M,Gong, P,Zhang, Y,&Zhang, F.(2017).Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles.LUMINESCENCE,32(4),618-624.
MLA Du, QQG,et al."Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles".LUMINESCENCE 32.4(2017):618-624.
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