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CAS OpenIR  > 中科院上海应用物理研究所2011-2017年  > 期刊论文
题名:
Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles
作者: Du, QQG; Li, WR; Yuan, M; Gong, P; Zhang, Y; Zhang, F
刊名: LUMINESCENCE
出版日期: 2017
卷号: 32, 期号:4, 页码:618-624
关键词: amphiphilic polymer ; binding constant ; G-actin ; gold nanoparticle ; photoluminescence quenching
DOI: 10.1002/bio.3229
文章类型: 期刊论文
英文摘要: Physisorptions occurs everywhere and constantly in living organisms and between nanomaterials and biomolecules. In this study, one of the most important proteins, G-actin, was selected to investigate its bio-nano physisorption with a model nanoparticle coated with a amphiphilic polymer. Using a photoluminescence quenching method, both the binding constant and the Hill constant were determined as 1.79x10(7)M(-1) and 0.84, respectively. Thermodynamic calculations proved that such a physisorption was a spontaneous procedure. The physisorption-mediated protein-nanoparticle conjugates were robust enough to resist gel electrophoresis, and protein conformation was kept intact, as revealed using circular dichroism. This conjugate might be a promising candidate for nanofabrication or could play a significant role in actin-related bioactivities.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000401144200020
ISSN号: 1522-7235
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sinap.ac.cn/handle/331007/27455
Appears in Collections:中科院上海应用物理研究所2011-2017年_期刊论文

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Recommended Citation:
Du, QQG,Li, WR,Yuan, M,et al. Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles[J]. LUMINESCENCE,2017-01-01,32(4):618-624.
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文件名: Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles.pdf
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