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| Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles | |
| Du, QQG; Li, WR; Yuan, M; Gong, P; Zhang, Y; Zhang, F | |
| 2017 | |
| Source Publication | LUMINESCENCE
 |
| ISSN | 1522-7235 |
| Volume | 32Issue:4Pages:618-624 |
| Subtype | 期刊论文 |
| Abstract | Physisorptions occurs everywhere and constantly in living organisms and between nanomaterials and biomolecules. In this study, one of the most important proteins, G-actin, was selected to investigate its bio-nano physisorption with a model nanoparticle coated with a amphiphilic polymer. Using a photoluminescence quenching method, both the binding constant and the Hill constant were determined as 1.79x10(7)M(-1) and 0.84, respectively. Thermodynamic calculations proved that such a physisorption was a spontaneous procedure. The physisorption-mediated protein-nanoparticle conjugates were robust enough to resist gel electrophoresis, and protein conformation was kept intact, as revealed using circular dichroism. This conjugate might be a promising candidate for nanofabrication or could play a significant role in actin-related bioactivities. |
| Keyword | Amphiphilic Polymer Binding Constant G-actin Gold Nanoparticle Photoluminescence Quenching |
| DOI | 10.1002/bio.3229 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000401144200020 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/27455 |
| Collection | 中科院上海应用物理研究所2011-2018年 |
| Recommended Citation GB/T 7714 | Du, QQG,Li, WR,Yuan, M,et al. Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles[J]. LUMINESCENCE,2017,32(4):618-624. |
| APA | Du, QQG,Li, WR,Yuan, M,Gong, P,Zhang, Y,&Zhang, F.(2017).Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles.LUMINESCENCE,32(4),618-624. |
| MLA | Du, QQG,et al."Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles".LUMINESCENCE 32.4(2017):618-624. |
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| Study on physisorpti（431KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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