CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
Bubble-Mediated Ultrasensitive Multiplex Detection of Metal Ions in Three-Dimensional DNA Nanostructure-Encoded Microchannels
Qu, XM; Yang, F; Chen, H; Li, J; Zhang, HB; Zhang, GJ; Li, L; Wang, LH; Song, SP; Tian, Y; Pei, H
2017
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume9Issue:19Pages:16026-16034
Subtype期刊论文
AbstractThe development of rapid and sensitive point-of-test devices for on-site monitoring of heavy-metal contamination has great scientific and technological importance. However, developing fast, inexpensive, and sensitive microarray sensors to achieve such a goal remains challenging. In this work, we present a DNA-nanostructured microarray (DNM) with a tubular three-dimensional sensing surface and an ordered nanotopography. This microarray enables enhanced molecular interaction toward the rapid and sensitive multiplex detection of heavy-metal ions. In our design, the use of DNA tetrahedral-structured probes engineers the sensing interface with spatially resolved and density-tunable sensing spots that improve the microconfined molecular recognition. A bubble-mediated shuttle reaction was used inside the DNM-functionalized microchannel to improve the target-capturing efficiency. Using this novel DNM biosensor, the sensitive and selective detection of multiple heavy-metal ions (i.e., Hg2+, Ag+, and Pb2+) was achieved within 5 min, the detection limit was down to 10, 10, and 20 nM for Hg2+, Ag+, and Pb2+, respectively. The feasibility of our DNM sensor was further demonstrated by probing heavy-metal ions in real water samples with a direct optical readout. Beyond metal ions, this unique DNM sensor can easily be extended to in vitro bioassays and clinical diagnostics.
KeywordGold Nanoparticles Energy-transfer Colorimetric Detection Microarray Analysis Injection-analysis Platform Aptamer Chip Hg2++ Oligonucleotide
DOI10.1021/acsami.7b03645
WOS KeywordGOLD NANOPARTICLES ; ENERGY-TRANSFER ; COLORIMETRIC DETECTION ; MICROARRAY ANALYSIS ; INJECTION-ANALYSIS ; PLATFORM ; APTAMER ; CHIP ; HG2+ ; OLIGONUCLEOTIDE
Indexed BySCI
Language英语
WOS IDWOS:000401782500013
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/28567
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Qu, XM,Yang, F,Chen, H,et al. Bubble-Mediated Ultrasensitive Multiplex Detection of Metal Ions in Three-Dimensional DNA Nanostructure-Encoded Microchannels[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(19):16026-16034.
APA Qu, XM.,Yang, F.,Chen, H.,Li, J.,Zhang, HB.,...&Pei, H.(2017).Bubble-Mediated Ultrasensitive Multiplex Detection of Metal Ions in Three-Dimensional DNA Nanostructure-Encoded Microchannels.ACS APPLIED MATERIALS & INTERFACES,9(19),16026-16034.
MLA Qu, XM,et al."Bubble-Mediated Ultrasensitive Multiplex Detection of Metal Ions in Three-Dimensional DNA Nanostructure-Encoded Microchannels".ACS APPLIED MATERIALS & INTERFACES 9.19(2017):16026-16034.
Files in This Item: Download All
File Name/Size DocType Version Access License
Bubble-Mediated Ultr(3228KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Qu, XM]'s Articles
[Yang, F]'s Articles
[Chen, H]'s Articles
Baidu academic
Similar articles in Baidu academic
[Qu, XM]'s Articles
[Yang, F]'s Articles
[Chen, H]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Qu, XM]'s Articles
[Yang, F]'s Articles
[Chen, H]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Bubble-Mediated Ultrasensitive Multiplex Detection of Metal Ions in Three-Dimensional DNA Nanostructure-Encoded Microchannels.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.