DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis
Song, P; Ye, DK; Zuo, XL; Li, J; Wang, JB; Liu, HJ; Hwang, MT; Chao, J; Su, S; Wang, LH; Shi, JY; Wang, LH; Huang, W; La, R; Fan, CH
2017
发表期刊NANO LETTERS
ISSN1530-6984
卷号17期号:9页码:5193-5198
文章类型期刊论文
摘要Circulating tumor cells (CTCs) contain molecular information on the primary tumor and can be used for predictive cancer diagnostics. Capturing rare live CTCs and their quantification in whole blood remain technically challenging. Here we report an aptamer-trigger clamped hybridization chain reaction (atcHCR) method for in situ identification and subsequent cloaking/decloaking of CTCs by porous DNA hydrogels. These decloaked CTCs were then used for live cell analysis. In our design, a DNA staple strand with aptamer-toehold biblocks specifically recognizes epithelial cell adhesion molecule (EpCAM) on the CTC surface that triggers subsequent atcHCR via toehold-initiated branch migration. Porous DNA hydrogel based-cloaking of single/cluster of CTCs allows capturing of living CTCs directly with minimal cell damage. The ability to identify a low number of CTCs in whole blood by DNA hydrogel cloaking would allow high sensitivity and specificity for diagnosis in clinically relevant settings. More significantly, decloaking of CTCs using controlled and defined chemical stimuli can release living CTCs without damages for subsequent culture and live cell analysis. We expect this liquid biopsy tool to open new powerful and effective routes for cancer diagnostics and therapeutics.
关键词Hybridization Chain-reaction Cancer-cells Capture Release Nanostructures Selection Platform Fate Chip
DOI10.1021/acs.nanolett.7b01006
关键词[WOS]HYBRIDIZATION CHAIN-REACTION ; CANCER-CELLS ; CAPTURE ; RELEASE ; NANOSTRUCTURES ; SELECTION ; PLATFORM ; FATE ; CHIP
收录类别SCI
语种英语
WOS记录号WOS:000411043500003
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28614
专题中科院上海应用物理研究所2011-2018年
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Song, P,Ye, DK,Zuo, XL,et al. DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis[J]. NANO LETTERS,2017,17(9):5193-5198.
APA Song, P.,Ye, DK.,Zuo, XL.,Li, J.,Wang, JB.,...&Fan, CH.(2017).DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis.NANO LETTERS,17(9),5193-5198.
MLA Song, P,et al."DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis".NANO LETTERS 17.9(2017):5193-5198.
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