CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis
Song, P; Ye, DK; Zuo, XL; Li, J; Wang, JB; Liu, HJ; Hwang, MT; Chao, J; Su, S; Wang, LH; Shi, JY; Wang, LH; Huang, W; La, R; Fan, CH
2017
Source PublicationNANO LETTERS
ISSN1530-6984
Volume17Issue:9Pages:5193-5198
Subtype期刊论文
AbstractCirculating tumor cells (CTCs) contain molecular information on the primary tumor and can be used for predictive cancer diagnostics. Capturing rare live CTCs and their quantification in whole blood remain technically challenging. Here we report an aptamer-trigger clamped hybridization chain reaction (atcHCR) method for in situ identification and subsequent cloaking/decloaking of CTCs by porous DNA hydrogels. These decloaked CTCs were then used for live cell analysis. In our design, a DNA staple strand with aptamer-toehold biblocks specifically recognizes epithelial cell adhesion molecule (EpCAM) on the CTC surface that triggers subsequent atcHCR via toehold-initiated branch migration. Porous DNA hydrogel based-cloaking of single/cluster of CTCs allows capturing of living CTCs directly with minimal cell damage. The ability to identify a low number of CTCs in whole blood by DNA hydrogel cloaking would allow high sensitivity and specificity for diagnosis in clinically relevant settings. More significantly, decloaking of CTCs using controlled and defined chemical stimuli can release living CTCs without damages for subsequent culture and live cell analysis. We expect this liquid biopsy tool to open new powerful and effective routes for cancer diagnostics and therapeutics.
KeywordHybridization Chain-reaction Cancer-cells Capture Release Nanostructures Selection Platform Fate Chip
DOI10.1021/acs.nanolett.7b01006
WOS KeywordHYBRIDIZATION CHAIN-REACTION ; CANCER-CELLS ; CAPTURE ; RELEASE ; NANOSTRUCTURES ; SELECTION ; PLATFORM ; FATE ; CHIP
Indexed BySCI
Language英语
WOS IDWOS:000411043500003
Citation statistics
Cited Times:43[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/28614
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Song, P,Ye, DK,Zuo, XL,et al. DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis[J]. NANO LETTERS,2017,17(9):5193-5198.
APA Song, P.,Ye, DK.,Zuo, XL.,Li, J.,Wang, JB.,...&Fan, CH.(2017).DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis.NANO LETTERS,17(9),5193-5198.
MLA Song, P,et al."DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis".NANO LETTERS 17.9(2017):5193-5198.
Files in This Item: Download All
File Name/Size DocType Version Access License
DNA Hydrogel with Ap(5518KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Song, P]'s Articles
[Ye, DK]'s Articles
[Zuo, XL]'s Articles
Baidu academic
Similar articles in Baidu academic
[Song, P]'s Articles
[Ye, DK]'s Articles
[Zuo, XL]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Song, P]'s Articles
[Ye, DK]'s Articles
[Zuo, XL]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.