CAS OpenIR  > 中科院上海应用物理研究所2011-2018年
DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection
Qi, L; Xiao, MS; Wang, XW; Wang, C; Wang, LH; Song, SP; Qu, XM; Li, L; Shi, JY; Pei, H
2017
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
Volume89Issue:18Pages:9850-9856
Subtype期刊论文
AbstractThe development of highly sensitive and selective methods for the detection of microRNA (miRNA) has attracted tremendous attention because of its importance in fundamental biological studies and diagnostic applications. In this work, we develop DNA-encoded Raman-active anisotropic nanoparticles modified origami paper analytical devices (oPADs) for rapid, highly sensitive, and specific miRNA detection. The Raman-active anisotropic nanoparticles were prepared using 10-mer oligo-A, -T, -C, and -G to mediate the growth of Ag cubic seeds into Ag nanoparticles (AgNPs) with different morphologies. The resulting AgNPs were further encoded with DNA probes to serve as effective surface-enhanced Raman scattering (SERS) probes. The analytical device was then fabricated on a single piece of SERS probes loaded paper-based substrate and assembled based on the principles of origami. The addition of the target analyte amplifies the Raman signals on DNA-encoded AgNPs through a target-dependent, sequence specific DNA hybridization assembly. This simple and low-cost analytical device is generic and applicable to a variety of miRNAs, allowing detection sensitivity down to 1 pM and assay time within 15 min, and therefore holds promising applications in point-of-care diagnostics.
KeywordSignal Amplification Sensitive Detection Ultrasensitive Detection Living Cells Small Rnas Sers Cancer Scattering Platform Paper
DOI10.1021/acs.analchem.7b01861
WOS KeywordSIGNAL AMPLIFICATION ; SENSITIVE DETECTION ; ULTRASENSITIVE DETECTION ; LIVING CELLS ; SMALL RNAS ; SERS ; CANCER ; SCATTERING ; PLATFORM ; PAPER
Indexed BySCI
Language英语
WOS IDWOS:000411549100035
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/28616
Collection中科院上海应用物理研究所2011-2018年
Recommended Citation
GB/T 7714
Qi, L,Xiao, MS,Wang, XW,et al. DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection[J]. ANALYTICAL CHEMISTRY,2017,89(18):9850-9856.
APA Qi, L.,Xiao, MS.,Wang, XW.,Wang, C.,Wang, LH.,...&Pei, H.(2017).DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection.ANALYTICAL CHEMISTRY,89(18),9850-9856.
MLA Qi, L,et al."DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection".ANALYTICAL CHEMISTRY 89.18(2017):9850-9856.
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