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| DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection | |
| Qi, L; Xiao, MS; Wang, XW; Wang, C; Wang, LH; Song, SP; Qu, XM; Li, L; Shi, JY; Pei, H | |
| 2017 | |
| Source Publication | ANALYTICAL CHEMISTRY
 |
| ISSN | 0003-2700 |
| Volume | 89Issue:18Pages:9850-9856 |
| Subtype | 期刊论文 |
| Abstract | The development of highly sensitive and selective methods for the detection of microRNA (miRNA) has attracted tremendous attention because of its importance in fundamental biological studies and diagnostic applications. In this work, we develop DNA-encoded Raman-active anisotropic nanoparticles modified origami paper analytical devices (oPADs) for rapid, highly sensitive, and specific miRNA detection. The Raman-active anisotropic nanoparticles were prepared using 10-mer oligo-A, -T, -C, and -G to mediate the growth of Ag cubic seeds into Ag nanoparticles (AgNPs) with different morphologies. The resulting AgNPs were further encoded with DNA probes to serve as effective surface-enhanced Raman scattering (SERS) probes. The analytical device was then fabricated on a single piece of SERS probes loaded paper-based substrate and assembled based on the principles of origami. The addition of the target analyte amplifies the Raman signals on DNA-encoded AgNPs through a target-dependent, sequence specific DNA hybridization assembly. This simple and low-cost analytical device is generic and applicable to a variety of miRNAs, allowing detection sensitivity down to 1 pM and assay time within 15 min, and therefore holds promising applications in point-of-care diagnostics. |
| Keyword | Signal Amplification Sensitive Detection Ultrasensitive Detection Living Cells Small Rnas Sers Cancer Scattering Platform Paper |
| DOI | 10.1021/acs.analchem.7b01861 |
| Indexed By | SCI |
| WOS Keyword | SIGNAL AMPLIFICATION ; SENSITIVE DETECTION ; ULTRASENSITIVE DETECTION ; LIVING CELLS ; SMALL RNAS ; SERS ; CANCER ; SCATTERING ; PLATFORM ; PAPER |
| Language | 英语 |
| WOS ID | WOS:000411549100035 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/28616 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Qi, L,Xiao, MS,Wang, XW,et al. DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection[J]. ANALYTICAL CHEMISTRY,2017,89(18):9850-9856. |
| APA | Qi, L.,Xiao, MS.,Wang, XW.,Wang, C.,Wang, LH.,...&Pei, H.(2017).DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection.ANALYTICAL CHEMISTRY,89(18),9850-9856. |
| MLA | Qi, L,et al."DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection".ANALYTICAL CHEMISTRY 89.18(2017):9850-9856. |
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| DNA-Encoded Raman-Ac（2449KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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