DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection
Qi, L; Xiao, MS; Wang, XW; Wang, C; Wang, LH; Song, SP; Qu, XM; Li, L; Shi, JY; Pei, H
2017
发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
卷号89期号:18页码:9850-9856
文章类型期刊论文
摘要The development of highly sensitive and selective methods for the detection of microRNA (miRNA) has attracted tremendous attention because of its importance in fundamental biological studies and diagnostic applications. In this work, we develop DNA-encoded Raman-active anisotropic nanoparticles modified origami paper analytical devices (oPADs) for rapid, highly sensitive, and specific miRNA detection. The Raman-active anisotropic nanoparticles were prepared using 10-mer oligo-A, -T, -C, and -G to mediate the growth of Ag cubic seeds into Ag nanoparticles (AgNPs) with different morphologies. The resulting AgNPs were further encoded with DNA probes to serve as effective surface-enhanced Raman scattering (SERS) probes. The analytical device was then fabricated on a single piece of SERS probes loaded paper-based substrate and assembled based on the principles of origami. The addition of the target analyte amplifies the Raman signals on DNA-encoded AgNPs through a target-dependent, sequence specific DNA hybridization assembly. This simple and low-cost analytical device is generic and applicable to a variety of miRNAs, allowing detection sensitivity down to 1 pM and assay time within 15 min, and therefore holds promising applications in point-of-care diagnostics.
关键词Signal Amplification Sensitive Detection Ultrasensitive Detection Living Cells Small Rnas Sers Cancer Scattering Platform Paper
DOI10.1021/acs.analchem.7b01861
关键词[WOS]SIGNAL AMPLIFICATION ; SENSITIVE DETECTION ; ULTRASENSITIVE DETECTION ; LIVING CELLS ; SMALL RNAS ; SERS ; CANCER ; SCATTERING ; PLATFORM ; PAPER
收录类别SCI
语种英语
WOS记录号WOS:000411549100035
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28616
专题中科院上海应用物理研究所2011-2018年
推荐引用方式
GB/T 7714
Qi, L,Xiao, MS,Wang, XW,et al. DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection[J]. ANALYTICAL CHEMISTRY,2017,89(18):9850-9856.
APA Qi, L.,Xiao, MS.,Wang, XW.,Wang, C.,Wang, LH.,...&Pei, H.(2017).DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection.ANALYTICAL CHEMISTRY,89(18),9850-9856.
MLA Qi, L,et al."DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection".ANALYTICAL CHEMISTRY 89.18(2017):9850-9856.
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