Docking of Antibodies into the Cavities of DNA Origami Structures
Ouyang, XY; De Stefano, M; Krissanaprasit, A; Kodal, ALB; Rosen, CB; Liu, TQ; Helmig, S; Fan, CH; Gothelf, KV
2017
发表期刊ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
ISSN1433-7851
卷号56期号:46页码:14423-14427
文章类型期刊论文
摘要Immobilized antibodies are extensively employed for medical diagnostics, such as in enzyme-linked immuno-sorbent assays. Despite their widespread use, the ability to control the orientation of immobilized antibodies on surfaces is very limited. Herein, we report a method for the covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNAorigami structures. Two tris(NTA)modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysine residues. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) confirmed the efficient immobilization of the antibodies in the origami structures. This increased control over the orientation of antibodies in nanostructures and on surfaces has the potential to direct the interactions between antibodies and targets and to provide more regular surface assemblies of antibodies.
关键词Self-assembled Nanostructures Surface-plasmon Resonance Histidine-tagged Proteins Oriented Immobilization Igg Immobilization 3d Dna Purification Binding Shapes Arrays
DOI10.1002/anie.201706765
关键词[WOS]SELF-ASSEMBLED NANOSTRUCTURES ; SURFACE-PLASMON RESONANCE ; HISTIDINE-TAGGED PROTEINS ; ORIENTED IMMOBILIZATION ; IGG IMMOBILIZATION ; 3D DNA ; PURIFICATION ; BINDING ; SHAPES ; ARRAYS
收录类别SCI
语种英语
WOS记录号WOS:000414764600011
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28618
专题中科院上海应用物理研究所2011-2018年
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Ouyang, XY,De Stefano, M,Krissanaprasit, A,et al. Docking of Antibodies into the Cavities of DNA Origami Structures[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(46):14423-14427.
APA Ouyang, XY.,De Stefano, M.,Krissanaprasit, A.,Kodal, ALB.,Rosen, CB.,...&Gothelf, KV.(2017).Docking of Antibodies into the Cavities of DNA Origami Structures.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(46),14423-14427.
MLA Ouyang, XY,et al."Docking of Antibodies into the Cavities of DNA Origami Structures".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.46(2017):14423-14427.
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