Formation of a Mimetic Biomembrane from the Hydrophobic Protein Zein and Phospholipids: Structure and Application
Wang, LP; Gotoh, T; Wang, YZ; Kouyama, T; Wane, JY
2017
发表期刊JOURNAL OF PHYSICAL CHEMISTRY C
ISSN1932-7447
卷号121期号:36页码:19999-20006
文章类型期刊论文
摘要alpha-Zein, a storage protein in corn endosperm, could be purified easily and in large amounts. In this study, a-zein was incorporated into phospholipid cholesterol (PC Chol) liposomes. The maximal amount of alpha-zein incorporated in the liposome was 0.05% (mol/mol) and the PC:Zein molar ratio was near 2400. At this level of zein insertion, the phase transition temperature of the lipid bilayer was little affected, but the leakage of doxorubicin (DOX) from the PC-Chol liposome became obviously slower when alpha-zein was added at a higher temperature than the phase transition temperature. Cryogenic transmission electron micrographs of the PC-Chol-Zein liposome showed that adjacent membranes in multilamellar vesicles were often aligned at a regular interval of about 7 mn. Data from synchrotron small-angle X-ray scattering of the PC-Chol-Zein liposome indicated the formation of the multilamellar structure with an intermembrane interval of 7.2 nm, whereas no homogeneous membrane alignment was observed in the absence of zein. The present observation can be well explained by supposing that alpha-zein takes on such an elongated conformation that it penetrates through two adjacent membrane layers. This feature seems to be compatible with a recently proposed superhelical structural model of alpha-zein. Meanwhile, experiments with the fluorescent-labeled alpha-zein showed that the PC Chol Zein liposome could be uptaken by an intact cell and localized in some specialized area (possibly endosomes) within the cell instead of being diffusely distributed in the cell. Thus, the PC Chol Zein liposome seems to act as an interesting biomembrane model and may be applicable as a drug delivery system.
关键词Membrane-proteins Lipid-bilayers Alpha-zein Corn Protein In-vitro Phase Maize Cholesterol Channels Tissue
DOI10.1021/acs.jpcc.7b04573
关键词[WOS]MEMBRANE-PROTEINS ; LIPID-BILAYERS ; ALPHA-ZEIN ; CORN PROTEIN ; IN-VITRO ; PHASE ; MAIZE ; CHOLESTEROL ; CHANNELS ; TISSUE
收录类别SCI
语种英语
WOS记录号WOS:000411171800048
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28664
专题中科院上海应用物理研究所2011-2018年
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Wang, LP,Gotoh, T,Wang, YZ,et al. Formation of a Mimetic Biomembrane from the Hydrophobic Protein Zein and Phospholipids: Structure and Application[J]. JOURNAL OF PHYSICAL CHEMISTRY C,2017,121(36):19999-20006.
APA Wang, LP,Gotoh, T,Wang, YZ,Kouyama, T,&Wane, JY.(2017).Formation of a Mimetic Biomembrane from the Hydrophobic Protein Zein and Phospholipids: Structure and Application.JOURNAL OF PHYSICAL CHEMISTRY C,121(36),19999-20006.
MLA Wang, LP,et al."Formation of a Mimetic Biomembrane from the Hydrophobic Protein Zein and Phospholipids: Structure and Application".JOURNAL OF PHYSICAL CHEMISTRY C 121.36(2017):19999-20006.
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