Identification of a nanobody specific to a human pulmonary surfactant protein A (vol 7, 1412, 2017)
He, X; Wang, SM; Yin, ZF; Zhao, MM; Li, N; Yu, F; Wang, LS; Hu, Y; Du, YK; Du, SS; Li, Y; Wei, YR; Chen, SS; He, JH; Weng, D; Li, HP
2017
发表期刊SCIENTIFIC REPORTS
ISSN2045-2322
卷号7期号:-页码:-
文章类型期刊论文
摘要Nanobody (Nb) is a promising vector for targeted drug delivery. This study aims to identify an Nb that can specifically target the lung by binding human pulmonary surfactant protein A (SP-A). Human lung frozen tissue sections were used for 3 rounds of biospanning of our previously constructed Nb library for rat SP-A to establish a sub-library of Nb, which specifically bound human lung tissues. Phage-ELISA was performed to screen the sub-library to identify Nb4, which specifically bound human SP-A. The binding affinity Kd of Nb4 to recombinant human SP-A was 7.48 × 10−7 M. Nb4 (19 kDa) was stable at 30 °C–37 °C and pH 7.0–7.6 and specifically bound the SP-A in human lung tissue homogenates, human lung A549 cells, and human lung tissues, whereas didn’t react with human liver L-02 cells, kidney 293T cells, and human tissues from organs other than the lung. Nb4 accumulated in the lung of nude mice 5 minutes after a tail vein injection of Nb4 and was excreted 3 hours. Short-term exposure (one month) to Nb4 didn’t cause apparent liver and kidney toxicity in rats, whereas 3-month exposure resulted in mild liver and kidney injuries. Nb4 may be a promising vector to specifically deliver drugs to the lung.
DOI10.1038/s41598-017-12271-0
收录类别SCI
语种英语
WOS记录号WOS:000411978000017
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28685
专题中科院上海应用物理研究所2011-2018年
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He, X,Wang, SM,Yin, ZF,et al. Identification of a nanobody specific to a human pulmonary surfactant protein A (vol 7, 1412, 2017)[J]. SCIENTIFIC REPORTS,2017,7(-):-.
APA He, X.,Wang, SM.,Yin, ZF.,Zhao, MM.,Li, N.,...&Li, HP.(2017).Identification of a nanobody specific to a human pulmonary surfactant protein A (vol 7, 1412, 2017).SCIENTIFIC REPORTS,7(-),-.
MLA He, X,et al."Identification of a nanobody specific to a human pulmonary surfactant protein A (vol 7, 1412, 2017)".SCIENTIFIC REPORTS 7.-(2017):-.
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