Inert Gas Deactivates Protein Activity by Aggregation
Zhang, LJ; Zhang, YB; Cheng, J; Wang, L; Wang, XY; Zhang, M; Gao, Y; Hu, J; Zhang, XH; Lu, JH; Li, GH; Tai, RZ; Fang, HP
2017
发表期刊SCIENTIFIC REPORTS
ISSN2045-2322
卷号7期号:-页码:-
文章类型期刊论文
摘要Biologically inert gases play important roles in the biological functionality of proteins. However, researchers lack a full understanding of the effects of these gases since they are very chemically stable only weakly absorbed by biological tissues. By combining X-ray fluorescence, particle sizing and molecular dynamics (MD) simulations, this work shows that the aggregation of these inert gases near the hydrophobic active cavity of pepsin should lead to protein deactivation. Micro X-ray fluorescence spectra show that a pepsin solution can contain a high concentration of Xe or Kr after gassing, and that the gas concentrations decrease quickly with degassing time. Biological activity experiments indicate a reversible deactivation of the protein during this gassing and degassing. Meanwhile, the nanoparticle size measurements reveal a higher number of "nanoparticles" in gas-containing pepsin solution, also supporting the possible interaction between inert gases and the protein. Further, MD simulations indicate that gas molecules can aggregate into a tiny bubble shape near the hydrophobic active cavity of pepsin, suggesting a mechanism for reducing their biological function.
关键词Der-waals Complexes Xenon Binding Xe Myoglobin Pathways Nitrogen Cavity Kr Ionization
DOI10.1038/s41598-017-10678-3
关键词[WOS]DER-WAALS COMPLEXES ; XENON ; BINDING ; XE ; MYOGLOBIN ; PATHWAYS ; NITROGEN ; CAVITY ; KR ; IONIZATION
收录类别SCI
语种英语
WOS记录号WOS:000408781200035
引用统计
文献类型期刊论文
条目标识符http://ir.sinap.ac.cn/handle/331007/28697
专题中科院上海应用物理研究所2011-2018年
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GB/T 7714
Zhang, LJ,Zhang, YB,Cheng, J,et al. Inert Gas Deactivates Protein Activity by Aggregation[J]. SCIENTIFIC REPORTS,2017,7(-):-.
APA Zhang, LJ.,Zhang, YB.,Cheng, J.,Wang, L.,Wang, XY.,...&Fang, HP.(2017).Inert Gas Deactivates Protein Activity by Aggregation.SCIENTIFIC REPORTS,7(-),-.
MLA Zhang, LJ,et al."Inert Gas Deactivates Protein Activity by Aggregation".SCIENTIFIC REPORTS 7.-(2017):-.
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